Cargando…
MiR-654-3p Suppresses Non-Small Cell Lung Cancer Tumourigenesis by Inhibiting PLK4
PURPOSE: MiR-654-3p plays important roles in many types of malignant tumours. However, the biological function of miR-654-3p in non-small cell lung cancer (NSCLC) remains unknown. In this study, the role of miR-654-3p in NSCLC was investigated. METHODS: qRT-PCR was used to evaluate the level of miR-...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7431606/ https://www.ncbi.nlm.nih.gov/pubmed/32884289 http://dx.doi.org/10.2147/OTT.S258616 |
_version_ | 1783571618079965184 |
---|---|
author | Pu, Jiang-tao Hu, Zhi Zhang, Deng-guo Zhang, Tao He, Kai-ming Dai, Tian-yang |
author_facet | Pu, Jiang-tao Hu, Zhi Zhang, Deng-guo Zhang, Tao He, Kai-ming Dai, Tian-yang |
author_sort | Pu, Jiang-tao |
collection | PubMed |
description | PURPOSE: MiR-654-3p plays important roles in many types of malignant tumours. However, the biological function of miR-654-3p in non-small cell lung cancer (NSCLC) remains unknown. In this study, the role of miR-654-3p in NSCLC was investigated. METHODS: qRT-PCR was used to evaluate the level of miR-654-3p in NSCLC tissues and cell lines, while Cell Counting Kit-8, Annexin V/propidium iodide dual staining or TUNEL staining were used to investigate proliferation and apoptosis of NSCLC cells. Luciferase assays and Western blotting were performed to validate potential targets of miR-654-3p. RESULTS: MiR-654-3p levels were significantly decreased in NSCLC patients and cell lines and were significantly correlated with the tumour size and tumour node metastasis stage of NSCLC patients. In A549 cells, miR-654-3p overexpression significantly increased apoptosis and inhibited growth both in vivo and in vitro, while downregulation of miR-654-3p had the opposite effects. In addition, polo-like kinase 4 (PLK4) was shown to be a target gene of miR-654-3p that is negatively regulated by miR-654-3p in A549 cells. Furthermore, PLK4 was observed to be highly expressed in NSCLC tissues and cells, and PLK4 overexpression abolished the inhibitory effects of miR-654-3p overexpression on NSCLC cell proliferation. Finally, the animal experiment results further demonstrated that miR-654-3p inhibits tumour growth and regulates PLK4 expression. CONCLUSION: Our results demonstrate that miR-654-3p functions as a growth-suppressing miRNA by targeting PLK4 in NSCLC. |
format | Online Article Text |
id | pubmed-7431606 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-74316062020-09-02 MiR-654-3p Suppresses Non-Small Cell Lung Cancer Tumourigenesis by Inhibiting PLK4 Pu, Jiang-tao Hu, Zhi Zhang, Deng-guo Zhang, Tao He, Kai-ming Dai, Tian-yang Onco Targets Ther Original Research PURPOSE: MiR-654-3p plays important roles in many types of malignant tumours. However, the biological function of miR-654-3p in non-small cell lung cancer (NSCLC) remains unknown. In this study, the role of miR-654-3p in NSCLC was investigated. METHODS: qRT-PCR was used to evaluate the level of miR-654-3p in NSCLC tissues and cell lines, while Cell Counting Kit-8, Annexin V/propidium iodide dual staining or TUNEL staining were used to investigate proliferation and apoptosis of NSCLC cells. Luciferase assays and Western blotting were performed to validate potential targets of miR-654-3p. RESULTS: MiR-654-3p levels were significantly decreased in NSCLC patients and cell lines and were significantly correlated with the tumour size and tumour node metastasis stage of NSCLC patients. In A549 cells, miR-654-3p overexpression significantly increased apoptosis and inhibited growth both in vivo and in vitro, while downregulation of miR-654-3p had the opposite effects. In addition, polo-like kinase 4 (PLK4) was shown to be a target gene of miR-654-3p that is negatively regulated by miR-654-3p in A549 cells. Furthermore, PLK4 was observed to be highly expressed in NSCLC tissues and cells, and PLK4 overexpression abolished the inhibitory effects of miR-654-3p overexpression on NSCLC cell proliferation. Finally, the animal experiment results further demonstrated that miR-654-3p inhibits tumour growth and regulates PLK4 expression. CONCLUSION: Our results demonstrate that miR-654-3p functions as a growth-suppressing miRNA by targeting PLK4 in NSCLC. Dove 2020-08-13 /pmc/articles/PMC7431606/ /pubmed/32884289 http://dx.doi.org/10.2147/OTT.S258616 Text en © 2020 Pu et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research Pu, Jiang-tao Hu, Zhi Zhang, Deng-guo Zhang, Tao He, Kai-ming Dai, Tian-yang MiR-654-3p Suppresses Non-Small Cell Lung Cancer Tumourigenesis by Inhibiting PLK4 |
title | MiR-654-3p Suppresses Non-Small Cell Lung Cancer Tumourigenesis by Inhibiting PLK4 |
title_full | MiR-654-3p Suppresses Non-Small Cell Lung Cancer Tumourigenesis by Inhibiting PLK4 |
title_fullStr | MiR-654-3p Suppresses Non-Small Cell Lung Cancer Tumourigenesis by Inhibiting PLK4 |
title_full_unstemmed | MiR-654-3p Suppresses Non-Small Cell Lung Cancer Tumourigenesis by Inhibiting PLK4 |
title_short | MiR-654-3p Suppresses Non-Small Cell Lung Cancer Tumourigenesis by Inhibiting PLK4 |
title_sort | mir-654-3p suppresses non-small cell lung cancer tumourigenesis by inhibiting plk4 |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7431606/ https://www.ncbi.nlm.nih.gov/pubmed/32884289 http://dx.doi.org/10.2147/OTT.S258616 |
work_keys_str_mv | AT pujiangtao mir6543psuppressesnonsmallcelllungcancertumourigenesisbyinhibitingplk4 AT huzhi mir6543psuppressesnonsmallcelllungcancertumourigenesisbyinhibitingplk4 AT zhangdengguo mir6543psuppressesnonsmallcelllungcancertumourigenesisbyinhibitingplk4 AT zhangtao mir6543psuppressesnonsmallcelllungcancertumourigenesisbyinhibitingplk4 AT hekaiming mir6543psuppressesnonsmallcelllungcancertumourigenesisbyinhibitingplk4 AT daitianyang mir6543psuppressesnonsmallcelllungcancertumourigenesisbyinhibitingplk4 |