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Genome-Wide DNA Methylation Profiles in Community Members Exposed to the World Trade Center Disaster

The primary goal of this pilot study was to assess feasibility of studies among local community members to address the hypothesis that complex exposures to the World Trade Center (WTC) dust and fumes resulted in long-term epigenetic changes. We enrolled 18 WTC-exposed cancer-free women from the WTC...

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Autores principales: Arslan, Alan A., Tuminello, Stephanie, Yang, Lei, Zhang, Yian, Durmus, Nedim, Snuderl, Matija, Heguy, Adriana, Zeleniuch-Jacquotte, Anne, Shao, Yongzhao, Reibman, Joan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7432006/
https://www.ncbi.nlm.nih.gov/pubmed/32751422
http://dx.doi.org/10.3390/ijerph17155493
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author Arslan, Alan A.
Tuminello, Stephanie
Yang, Lei
Zhang, Yian
Durmus, Nedim
Snuderl, Matija
Heguy, Adriana
Zeleniuch-Jacquotte, Anne
Shao, Yongzhao
Reibman, Joan
author_facet Arslan, Alan A.
Tuminello, Stephanie
Yang, Lei
Zhang, Yian
Durmus, Nedim
Snuderl, Matija
Heguy, Adriana
Zeleniuch-Jacquotte, Anne
Shao, Yongzhao
Reibman, Joan
author_sort Arslan, Alan A.
collection PubMed
description The primary goal of this pilot study was to assess feasibility of studies among local community members to address the hypothesis that complex exposures to the World Trade Center (WTC) dust and fumes resulted in long-term epigenetic changes. We enrolled 18 WTC-exposed cancer-free women from the WTC Environmental Health Center (WTC EHC) who agreed to donate blood samples during their standard clinical visits. As a reference WTC unexposed group, we randomly selected 24 age-matched cancer-free women from an existing prospective cohort who donated blood samples before 11 September 2001. The global DNA methylation analyses were performed using Illumina Infinium MethylationEpic arrays. Statistical analyses were performed using R Bioconductor package. Functional genomic analyses were done by mapping the top 5000 differentially expressed CpG sites to the Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathway database. Among cancer-free subjects, we observed substantial methylation differences between WTC-exposed and unexposed women. The top 15 differentially methylated gene probes included BCAS2, OSGIN1, BMI1, EEF1A2, SPTBN5, CHD8, CDCA7L, AIDA, DDN, SNORD45C, ZFAND6, ARHGEF7, UBXN8, USF1, and USP12. Several cancer-related pathways were enriched in the WTC-exposed subjects, including endocytosis, mitogen-activated protein kinase (MAPK), viral carcinogenesis, as well as Ras-associated protein-1 (Rap1) and mammalian target of rapamycin (mTOR) signaling. The study provides preliminary data on substantial differences in DNA methylation between WTC-exposed and unexposed populations that require validation in further studies.
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spelling pubmed-74320062020-08-24 Genome-Wide DNA Methylation Profiles in Community Members Exposed to the World Trade Center Disaster Arslan, Alan A. Tuminello, Stephanie Yang, Lei Zhang, Yian Durmus, Nedim Snuderl, Matija Heguy, Adriana Zeleniuch-Jacquotte, Anne Shao, Yongzhao Reibman, Joan Int J Environ Res Public Health Article The primary goal of this pilot study was to assess feasibility of studies among local community members to address the hypothesis that complex exposures to the World Trade Center (WTC) dust and fumes resulted in long-term epigenetic changes. We enrolled 18 WTC-exposed cancer-free women from the WTC Environmental Health Center (WTC EHC) who agreed to donate blood samples during their standard clinical visits. As a reference WTC unexposed group, we randomly selected 24 age-matched cancer-free women from an existing prospective cohort who donated blood samples before 11 September 2001. The global DNA methylation analyses were performed using Illumina Infinium MethylationEpic arrays. Statistical analyses were performed using R Bioconductor package. Functional genomic analyses were done by mapping the top 5000 differentially expressed CpG sites to the Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathway database. Among cancer-free subjects, we observed substantial methylation differences between WTC-exposed and unexposed women. The top 15 differentially methylated gene probes included BCAS2, OSGIN1, BMI1, EEF1A2, SPTBN5, CHD8, CDCA7L, AIDA, DDN, SNORD45C, ZFAND6, ARHGEF7, UBXN8, USF1, and USP12. Several cancer-related pathways were enriched in the WTC-exposed subjects, including endocytosis, mitogen-activated protein kinase (MAPK), viral carcinogenesis, as well as Ras-associated protein-1 (Rap1) and mammalian target of rapamycin (mTOR) signaling. The study provides preliminary data on substantial differences in DNA methylation between WTC-exposed and unexposed populations that require validation in further studies. MDPI 2020-07-30 2020-08 /pmc/articles/PMC7432006/ /pubmed/32751422 http://dx.doi.org/10.3390/ijerph17155493 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Arslan, Alan A.
Tuminello, Stephanie
Yang, Lei
Zhang, Yian
Durmus, Nedim
Snuderl, Matija
Heguy, Adriana
Zeleniuch-Jacquotte, Anne
Shao, Yongzhao
Reibman, Joan
Genome-Wide DNA Methylation Profiles in Community Members Exposed to the World Trade Center Disaster
title Genome-Wide DNA Methylation Profiles in Community Members Exposed to the World Trade Center Disaster
title_full Genome-Wide DNA Methylation Profiles in Community Members Exposed to the World Trade Center Disaster
title_fullStr Genome-Wide DNA Methylation Profiles in Community Members Exposed to the World Trade Center Disaster
title_full_unstemmed Genome-Wide DNA Methylation Profiles in Community Members Exposed to the World Trade Center Disaster
title_short Genome-Wide DNA Methylation Profiles in Community Members Exposed to the World Trade Center Disaster
title_sort genome-wide dna methylation profiles in community members exposed to the world trade center disaster
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7432006/
https://www.ncbi.nlm.nih.gov/pubmed/32751422
http://dx.doi.org/10.3390/ijerph17155493
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