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Metabolic Response of the Yeast Candida utilis During Enrichment in Selenium

Selenium (Se) was found to inhibit the growth of the yeast Candida utilis ATCC 9950. Cells cultured in 30 mg selenite/L supplemented medium could bind 1368 µg Se/g of dry weight in their structures. Increased accumulation of trehalose and glycogen was observed, which indicated cell response to stres...

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Autores principales: Kieliszek, Marek, Bierla, Katarzyna, Jiménez-Lamana, Javier, Kot, Anna Maria, Alcántara-Durán, Jaime, Piwowarek, Kamil, Błażejak, Stanisław, Szpunar, Joanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7432028/
https://www.ncbi.nlm.nih.gov/pubmed/32722488
http://dx.doi.org/10.3390/ijms21155287
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author Kieliszek, Marek
Bierla, Katarzyna
Jiménez-Lamana, Javier
Kot, Anna Maria
Alcántara-Durán, Jaime
Piwowarek, Kamil
Błażejak, Stanisław
Szpunar, Joanna
author_facet Kieliszek, Marek
Bierla, Katarzyna
Jiménez-Lamana, Javier
Kot, Anna Maria
Alcántara-Durán, Jaime
Piwowarek, Kamil
Błażejak, Stanisław
Szpunar, Joanna
author_sort Kieliszek, Marek
collection PubMed
description Selenium (Se) was found to inhibit the growth of the yeast Candida utilis ATCC 9950. Cells cultured in 30 mg selenite/L supplemented medium could bind 1368 µg Se/g of dry weight in their structures. Increased accumulation of trehalose and glycogen was observed, which indicated cell response to stress conditions. The activity of antioxidative enzymes (glutathione peroxidase, glutathione reductase, thioredoxin reductase, and glutathione S-transferase) was significantly higher than that of the control without Se addition. Most Se was bound to water-insoluble protein fraction; in addition, the yeast produced 20–30 nm Se nanoparticles (SeNPs). Part of Se was metabolized to selenomethionine (10%) and selenocysteine (20%). The HPLC-ESI-Orbitrap MS analysis showed the presence of five Se compounds combined with glutathione in the yeast. The obtained results form the basis for further research on the mechanisms of Se metabolism in yeast cells.
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spelling pubmed-74320282020-08-24 Metabolic Response of the Yeast Candida utilis During Enrichment in Selenium Kieliszek, Marek Bierla, Katarzyna Jiménez-Lamana, Javier Kot, Anna Maria Alcántara-Durán, Jaime Piwowarek, Kamil Błażejak, Stanisław Szpunar, Joanna Int J Mol Sci Article Selenium (Se) was found to inhibit the growth of the yeast Candida utilis ATCC 9950. Cells cultured in 30 mg selenite/L supplemented medium could bind 1368 µg Se/g of dry weight in their structures. Increased accumulation of trehalose and glycogen was observed, which indicated cell response to stress conditions. The activity of antioxidative enzymes (glutathione peroxidase, glutathione reductase, thioredoxin reductase, and glutathione S-transferase) was significantly higher than that of the control without Se addition. Most Se was bound to water-insoluble protein fraction; in addition, the yeast produced 20–30 nm Se nanoparticles (SeNPs). Part of Se was metabolized to selenomethionine (10%) and selenocysteine (20%). The HPLC-ESI-Orbitrap MS analysis showed the presence of five Se compounds combined with glutathione in the yeast. The obtained results form the basis for further research on the mechanisms of Se metabolism in yeast cells. MDPI 2020-07-25 /pmc/articles/PMC7432028/ /pubmed/32722488 http://dx.doi.org/10.3390/ijms21155287 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kieliszek, Marek
Bierla, Katarzyna
Jiménez-Lamana, Javier
Kot, Anna Maria
Alcántara-Durán, Jaime
Piwowarek, Kamil
Błażejak, Stanisław
Szpunar, Joanna
Metabolic Response of the Yeast Candida utilis During Enrichment in Selenium
title Metabolic Response of the Yeast Candida utilis During Enrichment in Selenium
title_full Metabolic Response of the Yeast Candida utilis During Enrichment in Selenium
title_fullStr Metabolic Response of the Yeast Candida utilis During Enrichment in Selenium
title_full_unstemmed Metabolic Response of the Yeast Candida utilis During Enrichment in Selenium
title_short Metabolic Response of the Yeast Candida utilis During Enrichment in Selenium
title_sort metabolic response of the yeast candida utilis during enrichment in selenium
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7432028/
https://www.ncbi.nlm.nih.gov/pubmed/32722488
http://dx.doi.org/10.3390/ijms21155287
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