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The In Vitro Effect of Prostaglandin E(2) and F(2α) on the Chemerin System in the Porcine Endometrium during Gestation
Chemerin belongs to the group of adipocyte-derived hormones known as adipokines, which are responsible mainly for the control of energy homeostasis. Adipokine exerts its influence through three receptors: Chemokine-like receptor 1 (CMKLR1), G protein-coupled receptor 1 (GPR1), and C-C motif chemokin...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7432131/ https://www.ncbi.nlm.nih.gov/pubmed/32717877 http://dx.doi.org/10.3390/ijms21155213 |
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author | Dobrzyn, Kamil Kiezun, Marta Zaobidna, Ewa Kisielewska, Katarzyna Rytelewska, Edyta Gudelska, Marlena Kopij, Grzegorz Bors, Kinga Szymanska, Karolina Kaminska, Barbara Kaminski, Tadeusz Smolinska, Nina |
author_facet | Dobrzyn, Kamil Kiezun, Marta Zaobidna, Ewa Kisielewska, Katarzyna Rytelewska, Edyta Gudelska, Marlena Kopij, Grzegorz Bors, Kinga Szymanska, Karolina Kaminska, Barbara Kaminski, Tadeusz Smolinska, Nina |
author_sort | Dobrzyn, Kamil |
collection | PubMed |
description | Chemerin belongs to the group of adipocyte-derived hormones known as adipokines, which are responsible mainly for the control of energy homeostasis. Adipokine exerts its influence through three receptors: Chemokine-like receptor 1 (CMKLR1), G protein-coupled receptor 1 (GPR1), and C-C motif chemokine receptor-like 2 (CCRL2). A growing body of evidence indicates that chemerin participates in the regulation of the female reproductive system. According to the literature, the expression of chemerin and its receptors in reproductive structures depends on the local hormonal milieu. The aim of this study was to investigate the in vitro effect of prostaglandins E(2) (PGE(2)) and F(2α) (PGF(2α)) on chemerin and chemerin receptor (chemerin system) mRNAs (qPCR) and proteins (ELISA, Western blotting) in endometrial tissue explants collected from early-pregnant gilts. Both PGE(2) and PGF(2α) significantly influenced the expression of the chemerin gene, hormone secretion, and the expression of chemerin receptor genes and proteins. The influence of both prostaglandins on the expression of the chemerin system varied between different stages of gestation. This is the first study to describe the modulatory effect of PGE(2) and PGF(2α) on the expression of the chemerin system in the porcine uterus during early gestation. |
format | Online Article Text |
id | pubmed-7432131 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-74321312020-08-24 The In Vitro Effect of Prostaglandin E(2) and F(2α) on the Chemerin System in the Porcine Endometrium during Gestation Dobrzyn, Kamil Kiezun, Marta Zaobidna, Ewa Kisielewska, Katarzyna Rytelewska, Edyta Gudelska, Marlena Kopij, Grzegorz Bors, Kinga Szymanska, Karolina Kaminska, Barbara Kaminski, Tadeusz Smolinska, Nina Int J Mol Sci Article Chemerin belongs to the group of adipocyte-derived hormones known as adipokines, which are responsible mainly for the control of energy homeostasis. Adipokine exerts its influence through three receptors: Chemokine-like receptor 1 (CMKLR1), G protein-coupled receptor 1 (GPR1), and C-C motif chemokine receptor-like 2 (CCRL2). A growing body of evidence indicates that chemerin participates in the regulation of the female reproductive system. According to the literature, the expression of chemerin and its receptors in reproductive structures depends on the local hormonal milieu. The aim of this study was to investigate the in vitro effect of prostaglandins E(2) (PGE(2)) and F(2α) (PGF(2α)) on chemerin and chemerin receptor (chemerin system) mRNAs (qPCR) and proteins (ELISA, Western blotting) in endometrial tissue explants collected from early-pregnant gilts. Both PGE(2) and PGF(2α) significantly influenced the expression of the chemerin gene, hormone secretion, and the expression of chemerin receptor genes and proteins. The influence of both prostaglandins on the expression of the chemerin system varied between different stages of gestation. This is the first study to describe the modulatory effect of PGE(2) and PGF(2α) on the expression of the chemerin system in the porcine uterus during early gestation. MDPI 2020-07-23 /pmc/articles/PMC7432131/ /pubmed/32717877 http://dx.doi.org/10.3390/ijms21155213 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Dobrzyn, Kamil Kiezun, Marta Zaobidna, Ewa Kisielewska, Katarzyna Rytelewska, Edyta Gudelska, Marlena Kopij, Grzegorz Bors, Kinga Szymanska, Karolina Kaminska, Barbara Kaminski, Tadeusz Smolinska, Nina The In Vitro Effect of Prostaglandin E(2) and F(2α) on the Chemerin System in the Porcine Endometrium during Gestation |
title | The In Vitro Effect of Prostaglandin E(2) and F(2α) on the Chemerin System in the Porcine Endometrium during Gestation |
title_full | The In Vitro Effect of Prostaglandin E(2) and F(2α) on the Chemerin System in the Porcine Endometrium during Gestation |
title_fullStr | The In Vitro Effect of Prostaglandin E(2) and F(2α) on the Chemerin System in the Porcine Endometrium during Gestation |
title_full_unstemmed | The In Vitro Effect of Prostaglandin E(2) and F(2α) on the Chemerin System in the Porcine Endometrium during Gestation |
title_short | The In Vitro Effect of Prostaglandin E(2) and F(2α) on the Chemerin System in the Porcine Endometrium during Gestation |
title_sort | in vitro effect of prostaglandin e(2) and f(2α) on the chemerin system in the porcine endometrium during gestation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7432131/ https://www.ncbi.nlm.nih.gov/pubmed/32717877 http://dx.doi.org/10.3390/ijms21155213 |
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