Cargando…

Identification and Characterization of Glycoproteins and Their Responsive Patterns upon Ethylene Stimulation in the Rubber Latex

Natural rubber is an important industrial material, which is obtained from the only commercially cultivated rubber tree, Hevea brasiliensis. In rubber latex production, ethylene has been extensively used as a stimulant. Recent research showed that post-translational modifications (PTMs) of latex pro...

Descripción completa

Detalles Bibliográficos
Autores principales: Yu, Li, Yuan, Boxuan, Wang, Lingling, Sun, Yong, Ding, Guohua, Souleymane, Ousmane Ahmat, Zhang, Xueyan, Xie, Quanliang, Wang, Xuchu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7432319/
https://www.ncbi.nlm.nih.gov/pubmed/32722428
http://dx.doi.org/10.3390/ijms21155282
_version_ 1783571770726416384
author Yu, Li
Yuan, Boxuan
Wang, Lingling
Sun, Yong
Ding, Guohua
Souleymane, Ousmane Ahmat
Zhang, Xueyan
Xie, Quanliang
Wang, Xuchu
author_facet Yu, Li
Yuan, Boxuan
Wang, Lingling
Sun, Yong
Ding, Guohua
Souleymane, Ousmane Ahmat
Zhang, Xueyan
Xie, Quanliang
Wang, Xuchu
author_sort Yu, Li
collection PubMed
description Natural rubber is an important industrial material, which is obtained from the only commercially cultivated rubber tree, Hevea brasiliensis. In rubber latex production, ethylene has been extensively used as a stimulant. Recent research showed that post-translational modifications (PTMs) of latex proteins, such as phosphorylation, glycosylation and ubiquitination, are crucial in natural rubber biosynthesis. In this study, comparative proteomics was performed to identify the glycosylated proteins in rubber latex treated with ethylene for different days. Combined with Pro-Q Glycoprotein gel staining and mass spectrometry techniques, we provided the first visual profiling of glycoproteomics of rubber latex and finally identified 144 glycosylated protein species, including 65 differentially accumulated proteins (DAPs) after treating with ethylene for three and/or five days. Gene Ontology (GO) functional annotation showed that these ethylene-responsive glycoproteins are mainly involved in cell parts, membrane components and metabolism. Pathway analysis demonstrated that these glycosylated rubber latex proteins are mainly involved in carbohydrate metabolism, energy metabolism, degradation function and cellular processes in rubber latex metabolism. Protein–protein interaction analysis revealed that these DAPs are mainly centered on acetyl-CoA acetyltransferase and hydroxymethylglutaryl-CoA synthase (HMGS) in the mevalonate pathway for natural rubber biosynthesis. In our glycoproteomics, three protein isoforms of HMGS2 were identified from rubber latex, and only one HMGS2 isoform was sharply increased in rubber latex by ethylene treatment for five days. Furthermore, the HbHMGS2 gene was over-expressed in a model rubber-producing grass Taraxacum Kok-saghyz and rubber content in the roots of transgenic rubber grass was significantly increased over that in the wild type plant, indicating HMGS2 is the key component for natural rubber production.
format Online
Article
Text
id pubmed-7432319
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-74323192020-08-24 Identification and Characterization of Glycoproteins and Their Responsive Patterns upon Ethylene Stimulation in the Rubber Latex Yu, Li Yuan, Boxuan Wang, Lingling Sun, Yong Ding, Guohua Souleymane, Ousmane Ahmat Zhang, Xueyan Xie, Quanliang Wang, Xuchu Int J Mol Sci Article Natural rubber is an important industrial material, which is obtained from the only commercially cultivated rubber tree, Hevea brasiliensis. In rubber latex production, ethylene has been extensively used as a stimulant. Recent research showed that post-translational modifications (PTMs) of latex proteins, such as phosphorylation, glycosylation and ubiquitination, are crucial in natural rubber biosynthesis. In this study, comparative proteomics was performed to identify the glycosylated proteins in rubber latex treated with ethylene for different days. Combined with Pro-Q Glycoprotein gel staining and mass spectrometry techniques, we provided the first visual profiling of glycoproteomics of rubber latex and finally identified 144 glycosylated protein species, including 65 differentially accumulated proteins (DAPs) after treating with ethylene for three and/or five days. Gene Ontology (GO) functional annotation showed that these ethylene-responsive glycoproteins are mainly involved in cell parts, membrane components and metabolism. Pathway analysis demonstrated that these glycosylated rubber latex proteins are mainly involved in carbohydrate metabolism, energy metabolism, degradation function and cellular processes in rubber latex metabolism. Protein–protein interaction analysis revealed that these DAPs are mainly centered on acetyl-CoA acetyltransferase and hydroxymethylglutaryl-CoA synthase (HMGS) in the mevalonate pathway for natural rubber biosynthesis. In our glycoproteomics, three protein isoforms of HMGS2 were identified from rubber latex, and only one HMGS2 isoform was sharply increased in rubber latex by ethylene treatment for five days. Furthermore, the HbHMGS2 gene was over-expressed in a model rubber-producing grass Taraxacum Kok-saghyz and rubber content in the roots of transgenic rubber grass was significantly increased over that in the wild type plant, indicating HMGS2 is the key component for natural rubber production. MDPI 2020-07-25 /pmc/articles/PMC7432319/ /pubmed/32722428 http://dx.doi.org/10.3390/ijms21155282 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yu, Li
Yuan, Boxuan
Wang, Lingling
Sun, Yong
Ding, Guohua
Souleymane, Ousmane Ahmat
Zhang, Xueyan
Xie, Quanliang
Wang, Xuchu
Identification and Characterization of Glycoproteins and Their Responsive Patterns upon Ethylene Stimulation in the Rubber Latex
title Identification and Characterization of Glycoproteins and Their Responsive Patterns upon Ethylene Stimulation in the Rubber Latex
title_full Identification and Characterization of Glycoproteins and Their Responsive Patterns upon Ethylene Stimulation in the Rubber Latex
title_fullStr Identification and Characterization of Glycoproteins and Their Responsive Patterns upon Ethylene Stimulation in the Rubber Latex
title_full_unstemmed Identification and Characterization of Glycoproteins and Their Responsive Patterns upon Ethylene Stimulation in the Rubber Latex
title_short Identification and Characterization of Glycoproteins and Their Responsive Patterns upon Ethylene Stimulation in the Rubber Latex
title_sort identification and characterization of glycoproteins and their responsive patterns upon ethylene stimulation in the rubber latex
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7432319/
https://www.ncbi.nlm.nih.gov/pubmed/32722428
http://dx.doi.org/10.3390/ijms21155282
work_keys_str_mv AT yuli identificationandcharacterizationofglycoproteinsandtheirresponsivepatternsuponethylenestimulationintherubberlatex
AT yuanboxuan identificationandcharacterizationofglycoproteinsandtheirresponsivepatternsuponethylenestimulationintherubberlatex
AT wanglingling identificationandcharacterizationofglycoproteinsandtheirresponsivepatternsuponethylenestimulationintherubberlatex
AT sunyong identificationandcharacterizationofglycoproteinsandtheirresponsivepatternsuponethylenestimulationintherubberlatex
AT dingguohua identificationandcharacterizationofglycoproteinsandtheirresponsivepatternsuponethylenestimulationintherubberlatex
AT souleymaneousmaneahmat identificationandcharacterizationofglycoproteinsandtheirresponsivepatternsuponethylenestimulationintherubberlatex
AT zhangxueyan identificationandcharacterizationofglycoproteinsandtheirresponsivepatternsuponethylenestimulationintherubberlatex
AT xiequanliang identificationandcharacterizationofglycoproteinsandtheirresponsivepatternsuponethylenestimulationintherubberlatex
AT wangxuchu identificationandcharacterizationofglycoproteinsandtheirresponsivepatternsuponethylenestimulationintherubberlatex