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Inhaled Argon Impedes Hepatic Regeneration after Ischemia/Reperfusion Injury in Rats

Organoprotective effects of noble gases are subject of current research. One important field of interest is the effect of noble gases on hepatic regenerative capacity. For the noble gas argon, promising studies demonstrated remarkable experimental effects in neuronal and renal cells. The aim of this...

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Autores principales: Schmitz, Sophia M., Dohmeier, Henriette, Stoppe, Christian, Alizai, Patrick H., Schipper, Sandra, Neumann, Ulf P., Coburn, Mark, Ulmer, Tom F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7432339/
https://www.ncbi.nlm.nih.gov/pubmed/32751707
http://dx.doi.org/10.3390/ijms21155457
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author Schmitz, Sophia M.
Dohmeier, Henriette
Stoppe, Christian
Alizai, Patrick H.
Schipper, Sandra
Neumann, Ulf P.
Coburn, Mark
Ulmer, Tom F.
author_facet Schmitz, Sophia M.
Dohmeier, Henriette
Stoppe, Christian
Alizai, Patrick H.
Schipper, Sandra
Neumann, Ulf P.
Coburn, Mark
Ulmer, Tom F.
author_sort Schmitz, Sophia M.
collection PubMed
description Organoprotective effects of noble gases are subject of current research. One important field of interest is the effect of noble gases on hepatic regenerative capacity. For the noble gas argon, promising studies demonstrated remarkable experimental effects in neuronal and renal cells. The aim of this study was to investigate the effects of argon on the regenerative capacity of the liver after ischemia/reperfusion injury (IRI). Male, Sprague-Dawley rats underwent hepatic IRI by clamping of the hepatic artery. Expression of hepatoproliferative genes (HGF, IL-1β, IL-6, TNF), cell cycle markers (BrdU, TUNEL, Ki-67), and liver enzymes (ALT, AST, Bilirubin, LDH) were assessed 3, 36, and 96 h after IRI. Expression of IL-1β and IL-6 was significantly higher after argon inhalation after 36 h (IL-1β 5.0 vs. 8.7 fold, p = 0.001; IL-6 9.6 vs. 19.1 fold, p = 0.05). Ki-67 was higher in the control group compared to the argon group after 36 h (214.0 vs. 38.7 positive cells/1000 hepatocytes, p = 0.045). Serum levels of AST and ALT did not differ significantly between groups. Our data indicate that argon inhalation has detrimental effects on liver regeneration after IRI as measured by elevated levels of the proinflammatory cytokines IL-1β and IL-6 after 36 h. In line with these results, Ki-67 is decreased in the argon group, indicating a negative effect on liver regeneration in argon inhalation.
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spelling pubmed-74323392020-08-24 Inhaled Argon Impedes Hepatic Regeneration after Ischemia/Reperfusion Injury in Rats Schmitz, Sophia M. Dohmeier, Henriette Stoppe, Christian Alizai, Patrick H. Schipper, Sandra Neumann, Ulf P. Coburn, Mark Ulmer, Tom F. Int J Mol Sci Article Organoprotective effects of noble gases are subject of current research. One important field of interest is the effect of noble gases on hepatic regenerative capacity. For the noble gas argon, promising studies demonstrated remarkable experimental effects in neuronal and renal cells. The aim of this study was to investigate the effects of argon on the regenerative capacity of the liver after ischemia/reperfusion injury (IRI). Male, Sprague-Dawley rats underwent hepatic IRI by clamping of the hepatic artery. Expression of hepatoproliferative genes (HGF, IL-1β, IL-6, TNF), cell cycle markers (BrdU, TUNEL, Ki-67), and liver enzymes (ALT, AST, Bilirubin, LDH) were assessed 3, 36, and 96 h after IRI. Expression of IL-1β and IL-6 was significantly higher after argon inhalation after 36 h (IL-1β 5.0 vs. 8.7 fold, p = 0.001; IL-6 9.6 vs. 19.1 fold, p = 0.05). Ki-67 was higher in the control group compared to the argon group after 36 h (214.0 vs. 38.7 positive cells/1000 hepatocytes, p = 0.045). Serum levels of AST and ALT did not differ significantly between groups. Our data indicate that argon inhalation has detrimental effects on liver regeneration after IRI as measured by elevated levels of the proinflammatory cytokines IL-1β and IL-6 after 36 h. In line with these results, Ki-67 is decreased in the argon group, indicating a negative effect on liver regeneration in argon inhalation. MDPI 2020-07-30 /pmc/articles/PMC7432339/ /pubmed/32751707 http://dx.doi.org/10.3390/ijms21155457 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Schmitz, Sophia M.
Dohmeier, Henriette
Stoppe, Christian
Alizai, Patrick H.
Schipper, Sandra
Neumann, Ulf P.
Coburn, Mark
Ulmer, Tom F.
Inhaled Argon Impedes Hepatic Regeneration after Ischemia/Reperfusion Injury in Rats
title Inhaled Argon Impedes Hepatic Regeneration after Ischemia/Reperfusion Injury in Rats
title_full Inhaled Argon Impedes Hepatic Regeneration after Ischemia/Reperfusion Injury in Rats
title_fullStr Inhaled Argon Impedes Hepatic Regeneration after Ischemia/Reperfusion Injury in Rats
title_full_unstemmed Inhaled Argon Impedes Hepatic Regeneration after Ischemia/Reperfusion Injury in Rats
title_short Inhaled Argon Impedes Hepatic Regeneration after Ischemia/Reperfusion Injury in Rats
title_sort inhaled argon impedes hepatic regeneration after ischemia/reperfusion injury in rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7432339/
https://www.ncbi.nlm.nih.gov/pubmed/32751707
http://dx.doi.org/10.3390/ijms21155457
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