Cargando…

Lipoteichoic Acid Accelerates Bone Healing by Enhancing Osteoblast Differentiation and Inhibiting Osteoclast Activation in a Mouse Model of Femoral Defects

Lipoteichoic acid (LTA) is a cell wall component of Gram-positive bacteria. Limited data suggest that LTA is beneficial for bone regeneration in vitro. Thus, we used a mouse model of femoral defects to explore the effects of LTA on bone healing in vivo. Micro-computed tomography analysis and double-...

Descripción completa

Detalles Bibliográficos
Autores principales: Hu, Chih-Chien, Chang, Chih-Hsiang, Hsiao, Yi-min, Chang, Yuhan, Wu, Ying-Yu, Ueng, Steve W. N., Chen, Mei-Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7432397/
https://www.ncbi.nlm.nih.gov/pubmed/32756396
http://dx.doi.org/10.3390/ijms21155550
_version_ 1783571788652871680
author Hu, Chih-Chien
Chang, Chih-Hsiang
Hsiao, Yi-min
Chang, Yuhan
Wu, Ying-Yu
Ueng, Steve W. N.
Chen, Mei-Feng
author_facet Hu, Chih-Chien
Chang, Chih-Hsiang
Hsiao, Yi-min
Chang, Yuhan
Wu, Ying-Yu
Ueng, Steve W. N.
Chen, Mei-Feng
author_sort Hu, Chih-Chien
collection PubMed
description Lipoteichoic acid (LTA) is a cell wall component of Gram-positive bacteria. Limited data suggest that LTA is beneficial for bone regeneration in vitro. Thus, we used a mouse model of femoral defects to explore the effects of LTA on bone healing in vivo. Micro-computed tomography analysis and double-fluorochrome labeling were utilized to examine whether LTA can accelerate dynamic bone formation in vivo. The effects of LTA on osteoblastogenesis and osteoclastogenesis were also studied in vitro. LTA treatment induced prompt bone bridge formation, rapid endochondral ossification, and accelerated healing of fractures in mice with femoral bone defects. In vitro, LTA directly enhanced indicators of osteogenic factor-induced MC3T3-E1 cell differentiation, including alkaline phosphatase activity, calcium deposition and osteopontin expression. LTA also inhibited osteoclast activation induced by receptor activator of nuclear factor-kappa B ligand. We identified six molecules that may be associated with LTA-accelerated bone healing: monocyte chemoattractant protein 1, chemokine (C-X-C motif) ligand 1, cystatin C, growth/differentiation factor 15, endostatin and neutrophil gelatinase-associated lipocalin. Finally, double-fluorochrome, dynamic-labeling data indicated that LTA significantly enhanced bone-formation rates in vivo. In conclusion, our findings suggest that LTA has promising bone-regeneration properties.
format Online
Article
Text
id pubmed-7432397
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-74323972020-08-24 Lipoteichoic Acid Accelerates Bone Healing by Enhancing Osteoblast Differentiation and Inhibiting Osteoclast Activation in a Mouse Model of Femoral Defects Hu, Chih-Chien Chang, Chih-Hsiang Hsiao, Yi-min Chang, Yuhan Wu, Ying-Yu Ueng, Steve W. N. Chen, Mei-Feng Int J Mol Sci Article Lipoteichoic acid (LTA) is a cell wall component of Gram-positive bacteria. Limited data suggest that LTA is beneficial for bone regeneration in vitro. Thus, we used a mouse model of femoral defects to explore the effects of LTA on bone healing in vivo. Micro-computed tomography analysis and double-fluorochrome labeling were utilized to examine whether LTA can accelerate dynamic bone formation in vivo. The effects of LTA on osteoblastogenesis and osteoclastogenesis were also studied in vitro. LTA treatment induced prompt bone bridge formation, rapid endochondral ossification, and accelerated healing of fractures in mice with femoral bone defects. In vitro, LTA directly enhanced indicators of osteogenic factor-induced MC3T3-E1 cell differentiation, including alkaline phosphatase activity, calcium deposition and osteopontin expression. LTA also inhibited osteoclast activation induced by receptor activator of nuclear factor-kappa B ligand. We identified six molecules that may be associated with LTA-accelerated bone healing: monocyte chemoattractant protein 1, chemokine (C-X-C motif) ligand 1, cystatin C, growth/differentiation factor 15, endostatin and neutrophil gelatinase-associated lipocalin. Finally, double-fluorochrome, dynamic-labeling data indicated that LTA significantly enhanced bone-formation rates in vivo. In conclusion, our findings suggest that LTA has promising bone-regeneration properties. MDPI 2020-08-03 /pmc/articles/PMC7432397/ /pubmed/32756396 http://dx.doi.org/10.3390/ijms21155550 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hu, Chih-Chien
Chang, Chih-Hsiang
Hsiao, Yi-min
Chang, Yuhan
Wu, Ying-Yu
Ueng, Steve W. N.
Chen, Mei-Feng
Lipoteichoic Acid Accelerates Bone Healing by Enhancing Osteoblast Differentiation and Inhibiting Osteoclast Activation in a Mouse Model of Femoral Defects
title Lipoteichoic Acid Accelerates Bone Healing by Enhancing Osteoblast Differentiation and Inhibiting Osteoclast Activation in a Mouse Model of Femoral Defects
title_full Lipoteichoic Acid Accelerates Bone Healing by Enhancing Osteoblast Differentiation and Inhibiting Osteoclast Activation in a Mouse Model of Femoral Defects
title_fullStr Lipoteichoic Acid Accelerates Bone Healing by Enhancing Osteoblast Differentiation and Inhibiting Osteoclast Activation in a Mouse Model of Femoral Defects
title_full_unstemmed Lipoteichoic Acid Accelerates Bone Healing by Enhancing Osteoblast Differentiation and Inhibiting Osteoclast Activation in a Mouse Model of Femoral Defects
title_short Lipoteichoic Acid Accelerates Bone Healing by Enhancing Osteoblast Differentiation and Inhibiting Osteoclast Activation in a Mouse Model of Femoral Defects
title_sort lipoteichoic acid accelerates bone healing by enhancing osteoblast differentiation and inhibiting osteoclast activation in a mouse model of femoral defects
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7432397/
https://www.ncbi.nlm.nih.gov/pubmed/32756396
http://dx.doi.org/10.3390/ijms21155550
work_keys_str_mv AT huchihchien lipoteichoicacidacceleratesbonehealingbyenhancingosteoblastdifferentiationandinhibitingosteoclastactivationinamousemodeloffemoraldefects
AT changchihhsiang lipoteichoicacidacceleratesbonehealingbyenhancingosteoblastdifferentiationandinhibitingosteoclastactivationinamousemodeloffemoraldefects
AT hsiaoyimin lipoteichoicacidacceleratesbonehealingbyenhancingosteoblastdifferentiationandinhibitingosteoclastactivationinamousemodeloffemoraldefects
AT changyuhan lipoteichoicacidacceleratesbonehealingbyenhancingosteoblastdifferentiationandinhibitingosteoclastactivationinamousemodeloffemoraldefects
AT wuyingyu lipoteichoicacidacceleratesbonehealingbyenhancingosteoblastdifferentiationandinhibitingosteoclastactivationinamousemodeloffemoraldefects
AT uengstevewn lipoteichoicacidacceleratesbonehealingbyenhancingosteoblastdifferentiationandinhibitingosteoclastactivationinamousemodeloffemoraldefects
AT chenmeifeng lipoteichoicacidacceleratesbonehealingbyenhancingosteoblastdifferentiationandinhibitingosteoclastactivationinamousemodeloffemoraldefects