Cargando…
Lipoteichoic Acid Accelerates Bone Healing by Enhancing Osteoblast Differentiation and Inhibiting Osteoclast Activation in a Mouse Model of Femoral Defects
Lipoteichoic acid (LTA) is a cell wall component of Gram-positive bacteria. Limited data suggest that LTA is beneficial for bone regeneration in vitro. Thus, we used a mouse model of femoral defects to explore the effects of LTA on bone healing in vivo. Micro-computed tomography analysis and double-...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7432397/ https://www.ncbi.nlm.nih.gov/pubmed/32756396 http://dx.doi.org/10.3390/ijms21155550 |
_version_ | 1783571788652871680 |
---|---|
author | Hu, Chih-Chien Chang, Chih-Hsiang Hsiao, Yi-min Chang, Yuhan Wu, Ying-Yu Ueng, Steve W. N. Chen, Mei-Feng |
author_facet | Hu, Chih-Chien Chang, Chih-Hsiang Hsiao, Yi-min Chang, Yuhan Wu, Ying-Yu Ueng, Steve W. N. Chen, Mei-Feng |
author_sort | Hu, Chih-Chien |
collection | PubMed |
description | Lipoteichoic acid (LTA) is a cell wall component of Gram-positive bacteria. Limited data suggest that LTA is beneficial for bone regeneration in vitro. Thus, we used a mouse model of femoral defects to explore the effects of LTA on bone healing in vivo. Micro-computed tomography analysis and double-fluorochrome labeling were utilized to examine whether LTA can accelerate dynamic bone formation in vivo. The effects of LTA on osteoblastogenesis and osteoclastogenesis were also studied in vitro. LTA treatment induced prompt bone bridge formation, rapid endochondral ossification, and accelerated healing of fractures in mice with femoral bone defects. In vitro, LTA directly enhanced indicators of osteogenic factor-induced MC3T3-E1 cell differentiation, including alkaline phosphatase activity, calcium deposition and osteopontin expression. LTA also inhibited osteoclast activation induced by receptor activator of nuclear factor-kappa B ligand. We identified six molecules that may be associated with LTA-accelerated bone healing: monocyte chemoattractant protein 1, chemokine (C-X-C motif) ligand 1, cystatin C, growth/differentiation factor 15, endostatin and neutrophil gelatinase-associated lipocalin. Finally, double-fluorochrome, dynamic-labeling data indicated that LTA significantly enhanced bone-formation rates in vivo. In conclusion, our findings suggest that LTA has promising bone-regeneration properties. |
format | Online Article Text |
id | pubmed-7432397 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-74323972020-08-24 Lipoteichoic Acid Accelerates Bone Healing by Enhancing Osteoblast Differentiation and Inhibiting Osteoclast Activation in a Mouse Model of Femoral Defects Hu, Chih-Chien Chang, Chih-Hsiang Hsiao, Yi-min Chang, Yuhan Wu, Ying-Yu Ueng, Steve W. N. Chen, Mei-Feng Int J Mol Sci Article Lipoteichoic acid (LTA) is a cell wall component of Gram-positive bacteria. Limited data suggest that LTA is beneficial for bone regeneration in vitro. Thus, we used a mouse model of femoral defects to explore the effects of LTA on bone healing in vivo. Micro-computed tomography analysis and double-fluorochrome labeling were utilized to examine whether LTA can accelerate dynamic bone formation in vivo. The effects of LTA on osteoblastogenesis and osteoclastogenesis were also studied in vitro. LTA treatment induced prompt bone bridge formation, rapid endochondral ossification, and accelerated healing of fractures in mice with femoral bone defects. In vitro, LTA directly enhanced indicators of osteogenic factor-induced MC3T3-E1 cell differentiation, including alkaline phosphatase activity, calcium deposition and osteopontin expression. LTA also inhibited osteoclast activation induced by receptor activator of nuclear factor-kappa B ligand. We identified six molecules that may be associated with LTA-accelerated bone healing: monocyte chemoattractant protein 1, chemokine (C-X-C motif) ligand 1, cystatin C, growth/differentiation factor 15, endostatin and neutrophil gelatinase-associated lipocalin. Finally, double-fluorochrome, dynamic-labeling data indicated that LTA significantly enhanced bone-formation rates in vivo. In conclusion, our findings suggest that LTA has promising bone-regeneration properties. MDPI 2020-08-03 /pmc/articles/PMC7432397/ /pubmed/32756396 http://dx.doi.org/10.3390/ijms21155550 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hu, Chih-Chien Chang, Chih-Hsiang Hsiao, Yi-min Chang, Yuhan Wu, Ying-Yu Ueng, Steve W. N. Chen, Mei-Feng Lipoteichoic Acid Accelerates Bone Healing by Enhancing Osteoblast Differentiation and Inhibiting Osteoclast Activation in a Mouse Model of Femoral Defects |
title | Lipoteichoic Acid Accelerates Bone Healing by Enhancing Osteoblast Differentiation and Inhibiting Osteoclast Activation in a Mouse Model of Femoral Defects |
title_full | Lipoteichoic Acid Accelerates Bone Healing by Enhancing Osteoblast Differentiation and Inhibiting Osteoclast Activation in a Mouse Model of Femoral Defects |
title_fullStr | Lipoteichoic Acid Accelerates Bone Healing by Enhancing Osteoblast Differentiation and Inhibiting Osteoclast Activation in a Mouse Model of Femoral Defects |
title_full_unstemmed | Lipoteichoic Acid Accelerates Bone Healing by Enhancing Osteoblast Differentiation and Inhibiting Osteoclast Activation in a Mouse Model of Femoral Defects |
title_short | Lipoteichoic Acid Accelerates Bone Healing by Enhancing Osteoblast Differentiation and Inhibiting Osteoclast Activation in a Mouse Model of Femoral Defects |
title_sort | lipoteichoic acid accelerates bone healing by enhancing osteoblast differentiation and inhibiting osteoclast activation in a mouse model of femoral defects |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7432397/ https://www.ncbi.nlm.nih.gov/pubmed/32756396 http://dx.doi.org/10.3390/ijms21155550 |
work_keys_str_mv | AT huchihchien lipoteichoicacidacceleratesbonehealingbyenhancingosteoblastdifferentiationandinhibitingosteoclastactivationinamousemodeloffemoraldefects AT changchihhsiang lipoteichoicacidacceleratesbonehealingbyenhancingosteoblastdifferentiationandinhibitingosteoclastactivationinamousemodeloffemoraldefects AT hsiaoyimin lipoteichoicacidacceleratesbonehealingbyenhancingosteoblastdifferentiationandinhibitingosteoclastactivationinamousemodeloffemoraldefects AT changyuhan lipoteichoicacidacceleratesbonehealingbyenhancingosteoblastdifferentiationandinhibitingosteoclastactivationinamousemodeloffemoraldefects AT wuyingyu lipoteichoicacidacceleratesbonehealingbyenhancingosteoblastdifferentiationandinhibitingosteoclastactivationinamousemodeloffemoraldefects AT uengstevewn lipoteichoicacidacceleratesbonehealingbyenhancingosteoblastdifferentiationandinhibitingosteoclastactivationinamousemodeloffemoraldefects AT chenmeifeng lipoteichoicacidacceleratesbonehealingbyenhancingosteoblastdifferentiationandinhibitingosteoclastactivationinamousemodeloffemoraldefects |