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Generation and Characterization of a Virulent Leptosphaeria maculans Isolate Carrying a Mutated AvrLm7 Gene Using the CRISPR/Cas9 System

Blackleg, caused by the fungal pathogen Leptosphaeria maculans, is the most important disease affecting canola (Brassica napus) crops worldwide. We employed the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) system to generate the mutant isolate umavr7 from...

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Detalles Bibliográficos
Autores principales: Zou, Zhongwei, Liu, Fei, Selin, Carrie, Fernando, W. G. Dilantha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7432424/
https://www.ncbi.nlm.nih.gov/pubmed/32849487
http://dx.doi.org/10.3389/fmicb.2020.01969