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Unusual Occurrence of Two Bona-Fide CCA-Adding Enzymes in Dictyostelium discoideum

Dictyostelium discoideum, the model organism for the evolutionary supergroup of Amoebozoa, is a social amoeba that, upon starvation, undergoes transition from a unicellular to a multicellular organism. In its genome, we identified two genes encoding for tRNA nucleotidyltransferases. Such pairs of tR...

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Autores principales: Erber, Lieselotte, Hoffmann, Anne, Fallmann, Jörg, Hagedorn, Monica, Hammann, Christian, Stadler, Peter F., Betat, Heike, Prohaska, Sonja, Mörl, Mario
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7432833/
https://www.ncbi.nlm.nih.gov/pubmed/32717856
http://dx.doi.org/10.3390/ijms21155210
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author Erber, Lieselotte
Hoffmann, Anne
Fallmann, Jörg
Hagedorn, Monica
Hammann, Christian
Stadler, Peter F.
Betat, Heike
Prohaska, Sonja
Mörl, Mario
author_facet Erber, Lieselotte
Hoffmann, Anne
Fallmann, Jörg
Hagedorn, Monica
Hammann, Christian
Stadler, Peter F.
Betat, Heike
Prohaska, Sonja
Mörl, Mario
author_sort Erber, Lieselotte
collection PubMed
description Dictyostelium discoideum, the model organism for the evolutionary supergroup of Amoebozoa, is a social amoeba that, upon starvation, undergoes transition from a unicellular to a multicellular organism. In its genome, we identified two genes encoding for tRNA nucleotidyltransferases. Such pairs of tRNA nucleotidyltransferases usually represent collaborating partial activities catalyzing CC- and A-addition to the tRNA 3′-end, respectively. In D. discoideum, however, both enzymes exhibit identical activities, representing bona-fide CCA-adding enzymes. Detailed characterization of the corresponding activities revealed that both enzymes seem to be essential and are regulated inversely during different developmental stages of D. discoideum. Intriguingly, this is the first description of two functionally equivalent CCA-adding enzymes using the same set of tRNAs and showing a similar distribution within the cell. This situation seems to be a common feature in Dictyostelia, as other members of this phylum carry similar pairs of tRNA nucleotidyltransferase genes in their genome.
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spelling pubmed-74328332020-08-27 Unusual Occurrence of Two Bona-Fide CCA-Adding Enzymes in Dictyostelium discoideum Erber, Lieselotte Hoffmann, Anne Fallmann, Jörg Hagedorn, Monica Hammann, Christian Stadler, Peter F. Betat, Heike Prohaska, Sonja Mörl, Mario Int J Mol Sci Article Dictyostelium discoideum, the model organism for the evolutionary supergroup of Amoebozoa, is a social amoeba that, upon starvation, undergoes transition from a unicellular to a multicellular organism. In its genome, we identified two genes encoding for tRNA nucleotidyltransferases. Such pairs of tRNA nucleotidyltransferases usually represent collaborating partial activities catalyzing CC- and A-addition to the tRNA 3′-end, respectively. In D. discoideum, however, both enzymes exhibit identical activities, representing bona-fide CCA-adding enzymes. Detailed characterization of the corresponding activities revealed that both enzymes seem to be essential and are regulated inversely during different developmental stages of D. discoideum. Intriguingly, this is the first description of two functionally equivalent CCA-adding enzymes using the same set of tRNAs and showing a similar distribution within the cell. This situation seems to be a common feature in Dictyostelia, as other members of this phylum carry similar pairs of tRNA nucleotidyltransferase genes in their genome. MDPI 2020-07-23 /pmc/articles/PMC7432833/ /pubmed/32717856 http://dx.doi.org/10.3390/ijms21155210 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Erber, Lieselotte
Hoffmann, Anne
Fallmann, Jörg
Hagedorn, Monica
Hammann, Christian
Stadler, Peter F.
Betat, Heike
Prohaska, Sonja
Mörl, Mario
Unusual Occurrence of Two Bona-Fide CCA-Adding Enzymes in Dictyostelium discoideum
title Unusual Occurrence of Two Bona-Fide CCA-Adding Enzymes in Dictyostelium discoideum
title_full Unusual Occurrence of Two Bona-Fide CCA-Adding Enzymes in Dictyostelium discoideum
title_fullStr Unusual Occurrence of Two Bona-Fide CCA-Adding Enzymes in Dictyostelium discoideum
title_full_unstemmed Unusual Occurrence of Two Bona-Fide CCA-Adding Enzymes in Dictyostelium discoideum
title_short Unusual Occurrence of Two Bona-Fide CCA-Adding Enzymes in Dictyostelium discoideum
title_sort unusual occurrence of two bona-fide cca-adding enzymes in dictyostelium discoideum
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7432833/
https://www.ncbi.nlm.nih.gov/pubmed/32717856
http://dx.doi.org/10.3390/ijms21155210
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