Cargando…

Time Length of Negativization and Cycle Threshold Values in 182 Healthcare Workers with Covid-19 in Milan, Italy: An Observational Cohort Study

Background: Coronavirus Disease 2019 (COVID-19) has rapidly spread worldwide, becoming an unprecedented public health emergency. Rapid detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) suspected cases is crucial to control the spread of infection. We aimed to evaluate the tim...

Descripción completa

Detalles Bibliográficos
Autores principales: Cariani, Lisa, Orena, Beatrice Silvia, Ambrogi, Federico, Gambazza, Simone, Maraschini, Anna, Dodaro, Antonella, Oggioni, Massimo, Orlandi, Annarosa, Pirrone, Alessia, Uceda Renteria, Sara, Bernazzani, Mara, Cantù, Anna Paola, Ceriotti, Ferruccio, Lunghi, Giovanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7432921/
https://www.ncbi.nlm.nih.gov/pubmed/32718008
http://dx.doi.org/10.3390/ijerph17155313
_version_ 1783571905889959936
author Cariani, Lisa
Orena, Beatrice Silvia
Ambrogi, Federico
Gambazza, Simone
Maraschini, Anna
Dodaro, Antonella
Oggioni, Massimo
Orlandi, Annarosa
Pirrone, Alessia
Uceda Renteria, Sara
Bernazzani, Mara
Cantù, Anna Paola
Ceriotti, Ferruccio
Lunghi, Giovanna
author_facet Cariani, Lisa
Orena, Beatrice Silvia
Ambrogi, Federico
Gambazza, Simone
Maraschini, Anna
Dodaro, Antonella
Oggioni, Massimo
Orlandi, Annarosa
Pirrone, Alessia
Uceda Renteria, Sara
Bernazzani, Mara
Cantù, Anna Paola
Ceriotti, Ferruccio
Lunghi, Giovanna
author_sort Cariani, Lisa
collection PubMed
description Background: Coronavirus Disease 2019 (COVID-19) has rapidly spread worldwide, becoming an unprecedented public health emergency. Rapid detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) suspected cases is crucial to control the spread of infection. We aimed to evaluate the time length of negativization from the onset of symptoms in healthcare workers (HCWs) with COVID-19, and to evaluate significant variations in cycle threshold (CT) values and gene positivity (E, RdRP, and N genes) among positive individuals who returned to work. Methods: We retrospectively analyzed a consecutive cohort of 182 SARS-CoV-2-positive HCWs in Milan, from 16 March to 30 April 2020. Nasopharyngeal swabs were tested by RT-PCR. Results: Asymptomatic HCWs were 17.6% (32/182), and 58 healed at 30 April 2020. The median time length of negativization was 4 weeks (35% of symptomatic versus 40% of asymptomatic HCWs). Four HCWs, healed at 30 April, turned positive within three weeks during controls set up in the work unit. Three-gene positivity had the greatest variability, and increasing CT values from single- to three-gene positivity among all age groups were observed. Conclusions: Self-isolation longer than two weeks and prolonged follow-up periods for the staff returning to work after COVID-19 could be the most suitable choices to counter the SARS-CoV-2 spread. Further studies are needed to investigate infectiousness profiles among positive individuals.
format Online
Article
Text
id pubmed-7432921
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-74329212020-08-28 Time Length of Negativization and Cycle Threshold Values in 182 Healthcare Workers with Covid-19 in Milan, Italy: An Observational Cohort Study Cariani, Lisa Orena, Beatrice Silvia Ambrogi, Federico Gambazza, Simone Maraschini, Anna Dodaro, Antonella Oggioni, Massimo Orlandi, Annarosa Pirrone, Alessia Uceda Renteria, Sara Bernazzani, Mara Cantù, Anna Paola Ceriotti, Ferruccio Lunghi, Giovanna Int J Environ Res Public Health Article Background: Coronavirus Disease 2019 (COVID-19) has rapidly spread worldwide, becoming an unprecedented public health emergency. Rapid detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) suspected cases is crucial to control the spread of infection. We aimed to evaluate the time length of negativization from the onset of symptoms in healthcare workers (HCWs) with COVID-19, and to evaluate significant variations in cycle threshold (CT) values and gene positivity (E, RdRP, and N genes) among positive individuals who returned to work. Methods: We retrospectively analyzed a consecutive cohort of 182 SARS-CoV-2-positive HCWs in Milan, from 16 March to 30 April 2020. Nasopharyngeal swabs were tested by RT-PCR. Results: Asymptomatic HCWs were 17.6% (32/182), and 58 healed at 30 April 2020. The median time length of negativization was 4 weeks (35% of symptomatic versus 40% of asymptomatic HCWs). Four HCWs, healed at 30 April, turned positive within three weeks during controls set up in the work unit. Three-gene positivity had the greatest variability, and increasing CT values from single- to three-gene positivity among all age groups were observed. Conclusions: Self-isolation longer than two weeks and prolonged follow-up periods for the staff returning to work after COVID-19 could be the most suitable choices to counter the SARS-CoV-2 spread. Further studies are needed to investigate infectiousness profiles among positive individuals. MDPI 2020-07-23 2020-08 /pmc/articles/PMC7432921/ /pubmed/32718008 http://dx.doi.org/10.3390/ijerph17155313 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cariani, Lisa
Orena, Beatrice Silvia
Ambrogi, Federico
Gambazza, Simone
Maraschini, Anna
Dodaro, Antonella
Oggioni, Massimo
Orlandi, Annarosa
Pirrone, Alessia
Uceda Renteria, Sara
Bernazzani, Mara
Cantù, Anna Paola
Ceriotti, Ferruccio
Lunghi, Giovanna
Time Length of Negativization and Cycle Threshold Values in 182 Healthcare Workers with Covid-19 in Milan, Italy: An Observational Cohort Study
title Time Length of Negativization and Cycle Threshold Values in 182 Healthcare Workers with Covid-19 in Milan, Italy: An Observational Cohort Study
title_full Time Length of Negativization and Cycle Threshold Values in 182 Healthcare Workers with Covid-19 in Milan, Italy: An Observational Cohort Study
title_fullStr Time Length of Negativization and Cycle Threshold Values in 182 Healthcare Workers with Covid-19 in Milan, Italy: An Observational Cohort Study
title_full_unstemmed Time Length of Negativization and Cycle Threshold Values in 182 Healthcare Workers with Covid-19 in Milan, Italy: An Observational Cohort Study
title_short Time Length of Negativization and Cycle Threshold Values in 182 Healthcare Workers with Covid-19 in Milan, Italy: An Observational Cohort Study
title_sort time length of negativization and cycle threshold values in 182 healthcare workers with covid-19 in milan, italy: an observational cohort study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7432921/
https://www.ncbi.nlm.nih.gov/pubmed/32718008
http://dx.doi.org/10.3390/ijerph17155313
work_keys_str_mv AT carianilisa timelengthofnegativizationandcyclethresholdvaluesin182healthcareworkerswithcovid19inmilanitalyanobservationalcohortstudy
AT orenabeatricesilvia timelengthofnegativizationandcyclethresholdvaluesin182healthcareworkerswithcovid19inmilanitalyanobservationalcohortstudy
AT ambrogifederico timelengthofnegativizationandcyclethresholdvaluesin182healthcareworkerswithcovid19inmilanitalyanobservationalcohortstudy
AT gambazzasimone timelengthofnegativizationandcyclethresholdvaluesin182healthcareworkerswithcovid19inmilanitalyanobservationalcohortstudy
AT maraschinianna timelengthofnegativizationandcyclethresholdvaluesin182healthcareworkerswithcovid19inmilanitalyanobservationalcohortstudy
AT dodaroantonella timelengthofnegativizationandcyclethresholdvaluesin182healthcareworkerswithcovid19inmilanitalyanobservationalcohortstudy
AT oggionimassimo timelengthofnegativizationandcyclethresholdvaluesin182healthcareworkerswithcovid19inmilanitalyanobservationalcohortstudy
AT orlandiannarosa timelengthofnegativizationandcyclethresholdvaluesin182healthcareworkerswithcovid19inmilanitalyanobservationalcohortstudy
AT pirronealessia timelengthofnegativizationandcyclethresholdvaluesin182healthcareworkerswithcovid19inmilanitalyanobservationalcohortstudy
AT ucedarenteriasara timelengthofnegativizationandcyclethresholdvaluesin182healthcareworkerswithcovid19inmilanitalyanobservationalcohortstudy
AT bernazzanimara timelengthofnegativizationandcyclethresholdvaluesin182healthcareworkerswithcovid19inmilanitalyanobservationalcohortstudy
AT cantuannapaola timelengthofnegativizationandcyclethresholdvaluesin182healthcareworkerswithcovid19inmilanitalyanobservationalcohortstudy
AT ceriottiferruccio timelengthofnegativizationandcyclethresholdvaluesin182healthcareworkerswithcovid19inmilanitalyanobservationalcohortstudy
AT lunghigiovanna timelengthofnegativizationandcyclethresholdvaluesin182healthcareworkerswithcovid19inmilanitalyanobservationalcohortstudy