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C-Fos expression in epileptogenic areas of nephropathic rats undergoing star fruit poisoning
PURPOSE: Studies have demonstrated that star fruit consumption by nephropathic patients triggers severe neurotoxic effects that can lead to convulsions or even death. Brain areas likely susceptible to star fruit poisoning have not been investigated. The objective of the present study was to map poss...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7433670/ https://www.ncbi.nlm.nih.gov/pubmed/32785417 http://dx.doi.org/10.1590/s0102-865020200070000005 |
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author | Santana, Renato Figueiredo da Silva, Layla Alves Rodrigues Achar, Eduardo Ballester, Gerson Ribeiro, Marcelo Augusto Fontenelle Ortiz, Sandra Regina Mota |
author_facet | Santana, Renato Figueiredo da Silva, Layla Alves Rodrigues Achar, Eduardo Ballester, Gerson Ribeiro, Marcelo Augusto Fontenelle Ortiz, Sandra Regina Mota |
author_sort | Santana, Renato Figueiredo |
collection | PubMed |
description | PURPOSE: Studies have demonstrated that star fruit consumption by nephropathic patients triggers severe neurotoxic effects that can lead to convulsions or even death. Brain areas likely susceptible to star fruit poisoning have not been investigated. The objective of the present study was to map possible epileptogenic areas susceptible to star fruit intoxication in nephropathic rats. METHODS: The study analyzed 25 rats (5 groups). Rats in the experimental group underwent bilateral ureteral obstruction surgery and orogastric gavages with star fruit juice. An electroencephalogram was used to confirm convulsive seizures. Urea and creatinine levels were used to confirm the uremia model. Immunohistochemical analysis was used to map cells with c-Fos protein (c-Fos+ cells) to identify brain areas with increased neuronal activity. Control groups included non-nephropathic and nephropathic rats that did not receive star fruit. RESULTS: A statistically significant increase (p<0.01) in c-Fos+ cells was noted in nephropathic animals receiving star fruit juice compared to control groups, in brain areas commonly related to epileptogenic neural circuits including the hippocampus, amygdala, rhinal cortex, anterior cingulate area, piriform area, and medial dorsal thalamus. CONCLUSION: These data corroborate the neurotoxic capacity of star fruit in nephropathic patients. |
format | Online Article Text |
id | pubmed-7433670 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
record_format | MEDLINE/PubMed |
spelling | pubmed-74336702020-08-25 C-Fos expression in epileptogenic areas of nephropathic rats undergoing star fruit poisoning Santana, Renato Figueiredo da Silva, Layla Alves Rodrigues Achar, Eduardo Ballester, Gerson Ribeiro, Marcelo Augusto Fontenelle Ortiz, Sandra Regina Mota Acta Cir Bras Original Article PURPOSE: Studies have demonstrated that star fruit consumption by nephropathic patients triggers severe neurotoxic effects that can lead to convulsions or even death. Brain areas likely susceptible to star fruit poisoning have not been investigated. The objective of the present study was to map possible epileptogenic areas susceptible to star fruit intoxication in nephropathic rats. METHODS: The study analyzed 25 rats (5 groups). Rats in the experimental group underwent bilateral ureteral obstruction surgery and orogastric gavages with star fruit juice. An electroencephalogram was used to confirm convulsive seizures. Urea and creatinine levels were used to confirm the uremia model. Immunohistochemical analysis was used to map cells with c-Fos protein (c-Fos+ cells) to identify brain areas with increased neuronal activity. Control groups included non-nephropathic and nephropathic rats that did not receive star fruit. RESULTS: A statistically significant increase (p<0.01) in c-Fos+ cells was noted in nephropathic animals receiving star fruit juice compared to control groups, in brain areas commonly related to epileptogenic neural circuits including the hippocampus, amygdala, rhinal cortex, anterior cingulate area, piriform area, and medial dorsal thalamus. CONCLUSION: These data corroborate the neurotoxic capacity of star fruit in nephropathic patients. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 2020-08-10 /pmc/articles/PMC7433670/ /pubmed/32785417 http://dx.doi.org/10.1590/s0102-865020200070000005 Text en https://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Santana, Renato Figueiredo da Silva, Layla Alves Rodrigues Achar, Eduardo Ballester, Gerson Ribeiro, Marcelo Augusto Fontenelle Ortiz, Sandra Regina Mota C-Fos expression in epileptogenic areas of nephropathic rats undergoing star fruit poisoning |
title | C-Fos expression in epileptogenic areas of nephropathic rats undergoing star fruit poisoning
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title_full | C-Fos expression in epileptogenic areas of nephropathic rats undergoing star fruit poisoning
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title_fullStr | C-Fos expression in epileptogenic areas of nephropathic rats undergoing star fruit poisoning
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title_full_unstemmed | C-Fos expression in epileptogenic areas of nephropathic rats undergoing star fruit poisoning
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title_short | C-Fos expression in epileptogenic areas of nephropathic rats undergoing star fruit poisoning
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title_sort | c-fos expression in epileptogenic areas of nephropathic rats undergoing star fruit poisoning |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7433670/ https://www.ncbi.nlm.nih.gov/pubmed/32785417 http://dx.doi.org/10.1590/s0102-865020200070000005 |
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