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Dynamic centriolar localization of Polo and Centrobin in early mitosis primes centrosome asymmetry

Centrosomes, the main microtubule organizing centers (MTOCs) of metazoan cells, contain an older “mother” and a younger “daughter” centriole. Stem cells either inherit the mother or daughter-centriole-containing centrosome, providing a possible mechanism for biased delivery of cell fate determinants...

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Autores principales: Gallaud, Emmanuel, Ramdas Nair, Anjana, Horsley, Nicole, Monnard, Arnaud, Singh, Priyanka, Pham, Tri Thanh, Salvador Garcia, David, Ferrand, Alexia, Cabernard, Clemens
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7433902/
https://www.ncbi.nlm.nih.gov/pubmed/32760088
http://dx.doi.org/10.1371/journal.pbio.3000762
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author Gallaud, Emmanuel
Ramdas Nair, Anjana
Horsley, Nicole
Monnard, Arnaud
Singh, Priyanka
Pham, Tri Thanh
Salvador Garcia, David
Ferrand, Alexia
Cabernard, Clemens
author_facet Gallaud, Emmanuel
Ramdas Nair, Anjana
Horsley, Nicole
Monnard, Arnaud
Singh, Priyanka
Pham, Tri Thanh
Salvador Garcia, David
Ferrand, Alexia
Cabernard, Clemens
author_sort Gallaud, Emmanuel
collection PubMed
description Centrosomes, the main microtubule organizing centers (MTOCs) of metazoan cells, contain an older “mother” and a younger “daughter” centriole. Stem cells either inherit the mother or daughter-centriole-containing centrosome, providing a possible mechanism for biased delivery of cell fate determinants. However, the mechanisms regulating centrosome asymmetry and biased centrosome segregation are unclear. Using 3D-structured illumination microscopy (3D-SIM) and live-cell imaging, we show in fly neural stem cells (neuroblasts) that the mitotic kinase Polo and its centriolar protein substrate Centrobin (Cnb) accumulate on the daughter centriole during mitosis, thereby generating molecularly distinct mother and daughter centrioles before interphase. Cnb’s asymmetric localization, potentially involving a direct relocalization mechanism, is regulated by Polo-mediated phosphorylation, whereas Polo’s daughter centriole enrichment requires both Wdr62 and Cnb. Based on optogenetic protein mislocalization experiments, we propose that the establishment of centriole asymmetry in mitosis primes biased interphase MTOC activity, necessary for correct spindle orientation.
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spelling pubmed-74339022020-08-25 Dynamic centriolar localization of Polo and Centrobin in early mitosis primes centrosome asymmetry Gallaud, Emmanuel Ramdas Nair, Anjana Horsley, Nicole Monnard, Arnaud Singh, Priyanka Pham, Tri Thanh Salvador Garcia, David Ferrand, Alexia Cabernard, Clemens PLoS Biol Research Article Centrosomes, the main microtubule organizing centers (MTOCs) of metazoan cells, contain an older “mother” and a younger “daughter” centriole. Stem cells either inherit the mother or daughter-centriole-containing centrosome, providing a possible mechanism for biased delivery of cell fate determinants. However, the mechanisms regulating centrosome asymmetry and biased centrosome segregation are unclear. Using 3D-structured illumination microscopy (3D-SIM) and live-cell imaging, we show in fly neural stem cells (neuroblasts) that the mitotic kinase Polo and its centriolar protein substrate Centrobin (Cnb) accumulate on the daughter centriole during mitosis, thereby generating molecularly distinct mother and daughter centrioles before interphase. Cnb’s asymmetric localization, potentially involving a direct relocalization mechanism, is regulated by Polo-mediated phosphorylation, whereas Polo’s daughter centriole enrichment requires both Wdr62 and Cnb. Based on optogenetic protein mislocalization experiments, we propose that the establishment of centriole asymmetry in mitosis primes biased interphase MTOC activity, necessary for correct spindle orientation. Public Library of Science 2020-08-06 /pmc/articles/PMC7433902/ /pubmed/32760088 http://dx.doi.org/10.1371/journal.pbio.3000762 Text en © 2020 Gallaud et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Gallaud, Emmanuel
Ramdas Nair, Anjana
Horsley, Nicole
Monnard, Arnaud
Singh, Priyanka
Pham, Tri Thanh
Salvador Garcia, David
Ferrand, Alexia
Cabernard, Clemens
Dynamic centriolar localization of Polo and Centrobin in early mitosis primes centrosome asymmetry
title Dynamic centriolar localization of Polo and Centrobin in early mitosis primes centrosome asymmetry
title_full Dynamic centriolar localization of Polo and Centrobin in early mitosis primes centrosome asymmetry
title_fullStr Dynamic centriolar localization of Polo and Centrobin in early mitosis primes centrosome asymmetry
title_full_unstemmed Dynamic centriolar localization of Polo and Centrobin in early mitosis primes centrosome asymmetry
title_short Dynamic centriolar localization of Polo and Centrobin in early mitosis primes centrosome asymmetry
title_sort dynamic centriolar localization of polo and centrobin in early mitosis primes centrosome asymmetry
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7433902/
https://www.ncbi.nlm.nih.gov/pubmed/32760088
http://dx.doi.org/10.1371/journal.pbio.3000762
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