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Association of viral load and physical status of HPV-16 with survival of patients with head and neck cancer

INTRODUCTION: Head and neck cancers (NHCs) are of multifaceted origins, and tobacco and alcohol are the primary risk factors. Currently, other factors associated with the genesis of these tumours are being considered, among these viral infections, especially human papillomavirus (HPV) infection. OBJ...

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Autores principales: Veitía, Dayahindara, Liuzzi, Juan, Ávila, Maira, Rodriguez, Idamelys, Toro, Felix, Correnti, María
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cancer Intelligence 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7434508/
https://www.ncbi.nlm.nih.gov/pubmed/32863876
http://dx.doi.org/10.3332/ecancer.2020.1082
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author Veitía, Dayahindara
Liuzzi, Juan
Ávila, Maira
Rodriguez, Idamelys
Toro, Felix
Correnti, María
author_facet Veitía, Dayahindara
Liuzzi, Juan
Ávila, Maira
Rodriguez, Idamelys
Toro, Felix
Correnti, María
author_sort Veitía, Dayahindara
collection PubMed
description INTRODUCTION: Head and neck cancers (NHCs) are of multifaceted origins, and tobacco and alcohol are the primary risk factors. Currently, other factors associated with the genesis of these tumours are being considered, among these viral infections, especially human papillomavirus (HPV) infection. OBJECTIVE: The objective was to evaluate HPV infection, HPV-16 E6 load and its physical status in patients with squamous cell carcinoma in the head and neck and evaluate its effects in the survival of these patients. METHODOLOGY: A total of 80 fresh biopsies of HNC were evaluated. The genetic material was extracted using the commercial kit QIAGEN. The detection and classification of HPV were carried out using INNO-LiPA, whereas the quantification and analysis of integration of the viral genome into the host cell were carried out using real-time PCR. RESULTS: The average age of the patients included was 60.34 ± 14.48 years, with a predominance of the male gender. The most frequent HPV infection was genotype 16 (52.8%), with an average of 10 copies of the HPV-16 E6/β-globin gene. Furthermore, an integration of the viral genome in the host cell was observed in 86% of cases with a statistically significant relationship between the location of the tumour and the viral load (p < 0.05). CONCLUSIONS: HPV-16 is the most common infection, and its physical status in the host cell is the determining factor in establishing response to treatment. However, more studies are needed to demonstrate the role of HPV infection in carcinogenesis.
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spelling pubmed-74345082020-08-28 Association of viral load and physical status of HPV-16 with survival of patients with head and neck cancer Veitía, Dayahindara Liuzzi, Juan Ávila, Maira Rodriguez, Idamelys Toro, Felix Correnti, María Ecancermedicalscience Research INTRODUCTION: Head and neck cancers (NHCs) are of multifaceted origins, and tobacco and alcohol are the primary risk factors. Currently, other factors associated with the genesis of these tumours are being considered, among these viral infections, especially human papillomavirus (HPV) infection. OBJECTIVE: The objective was to evaluate HPV infection, HPV-16 E6 load and its physical status in patients with squamous cell carcinoma in the head and neck and evaluate its effects in the survival of these patients. METHODOLOGY: A total of 80 fresh biopsies of HNC were evaluated. The genetic material was extracted using the commercial kit QIAGEN. The detection and classification of HPV were carried out using INNO-LiPA, whereas the quantification and analysis of integration of the viral genome into the host cell were carried out using real-time PCR. RESULTS: The average age of the patients included was 60.34 ± 14.48 years, with a predominance of the male gender. The most frequent HPV infection was genotype 16 (52.8%), with an average of 10 copies of the HPV-16 E6/β-globin gene. Furthermore, an integration of the viral genome in the host cell was observed in 86% of cases with a statistically significant relationship between the location of the tumour and the viral load (p < 0.05). CONCLUSIONS: HPV-16 is the most common infection, and its physical status in the host cell is the determining factor in establishing response to treatment. However, more studies are needed to demonstrate the role of HPV infection in carcinogenesis. Cancer Intelligence 2020-07-30 /pmc/articles/PMC7434508/ /pubmed/32863876 http://dx.doi.org/10.3332/ecancer.2020.1082 Text en © the authors; licensee ecancermedicalscience. http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Veitía, Dayahindara
Liuzzi, Juan
Ávila, Maira
Rodriguez, Idamelys
Toro, Felix
Correnti, María
Association of viral load and physical status of HPV-16 with survival of patients with head and neck cancer
title Association of viral load and physical status of HPV-16 with survival of patients with head and neck cancer
title_full Association of viral load and physical status of HPV-16 with survival of patients with head and neck cancer
title_fullStr Association of viral load and physical status of HPV-16 with survival of patients with head and neck cancer
title_full_unstemmed Association of viral load and physical status of HPV-16 with survival of patients with head and neck cancer
title_short Association of viral load and physical status of HPV-16 with survival of patients with head and neck cancer
title_sort association of viral load and physical status of hpv-16 with survival of patients with head and neck cancer
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7434508/
https://www.ncbi.nlm.nih.gov/pubmed/32863876
http://dx.doi.org/10.3332/ecancer.2020.1082
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