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Efficient ammonia production from food by-products by engineered Escherichia coli

Ammonia is used as a fertilizer for agriculture, chemical raw material, and carrier for transporting hydrogen, and with economic development, the demand for ammonia has increased. The Haber–Bosch process, which is the main method for producing ammonia, can produce ammonia with high efficiency. Howev...

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Autores principales: Tatemichi, Yuki, Kuroda, Kouichi, Nakahara, Takeharu, Ueda, Mitsuyoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7434829/
https://www.ncbi.nlm.nih.gov/pubmed/32809073
http://dx.doi.org/10.1186/s13568-020-01083-7
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author Tatemichi, Yuki
Kuroda, Kouichi
Nakahara, Takeharu
Ueda, Mitsuyoshi
author_facet Tatemichi, Yuki
Kuroda, Kouichi
Nakahara, Takeharu
Ueda, Mitsuyoshi
author_sort Tatemichi, Yuki
collection PubMed
description Ammonia is used as a fertilizer for agriculture, chemical raw material, and carrier for transporting hydrogen, and with economic development, the demand for ammonia has increased. The Haber–Bosch process, which is the main method for producing ammonia, can produce ammonia with high efficiency. However, since it consumes a large amount of fossil energy, it is necessary to develop an alternative method for producing ammonia with less environmental impact. Ammonia production from food by-products is an appealing production process owing to unused resource usage, including waste, and mild reaction conditions. However, when food by-products and biomass are used as feedstocks, impurities often reduce productivity. Using metabolic profiling, glucose was identified as a potential inhibitor of ammonia production from impure food by-products. We constructed the recombinant Escherichia coli, in which glucose uptake was reduced by ptsG gene disruption and amino acid catabolism was promoted by glnA gene disruption. Ammonia production efficiency from okara, a food by-product, was improved in this strain; 35.4 mM ammonia was produced (47% yield). This study might provide a strategy for efficient ammonia production from food by-products.
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spelling pubmed-74348292020-08-24 Efficient ammonia production from food by-products by engineered Escherichia coli Tatemichi, Yuki Kuroda, Kouichi Nakahara, Takeharu Ueda, Mitsuyoshi AMB Express Original Article Ammonia is used as a fertilizer for agriculture, chemical raw material, and carrier for transporting hydrogen, and with economic development, the demand for ammonia has increased. The Haber–Bosch process, which is the main method for producing ammonia, can produce ammonia with high efficiency. However, since it consumes a large amount of fossil energy, it is necessary to develop an alternative method for producing ammonia with less environmental impact. Ammonia production from food by-products is an appealing production process owing to unused resource usage, including waste, and mild reaction conditions. However, when food by-products and biomass are used as feedstocks, impurities often reduce productivity. Using metabolic profiling, glucose was identified as a potential inhibitor of ammonia production from impure food by-products. We constructed the recombinant Escherichia coli, in which glucose uptake was reduced by ptsG gene disruption and amino acid catabolism was promoted by glnA gene disruption. Ammonia production efficiency from okara, a food by-product, was improved in this strain; 35.4 mM ammonia was produced (47% yield). This study might provide a strategy for efficient ammonia production from food by-products. Springer Berlin Heidelberg 2020-08-18 /pmc/articles/PMC7434829/ /pubmed/32809073 http://dx.doi.org/10.1186/s13568-020-01083-7 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Original Article
Tatemichi, Yuki
Kuroda, Kouichi
Nakahara, Takeharu
Ueda, Mitsuyoshi
Efficient ammonia production from food by-products by engineered Escherichia coli
title Efficient ammonia production from food by-products by engineered Escherichia coli
title_full Efficient ammonia production from food by-products by engineered Escherichia coli
title_fullStr Efficient ammonia production from food by-products by engineered Escherichia coli
title_full_unstemmed Efficient ammonia production from food by-products by engineered Escherichia coli
title_short Efficient ammonia production from food by-products by engineered Escherichia coli
title_sort efficient ammonia production from food by-products by engineered escherichia coli
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7434829/
https://www.ncbi.nlm.nih.gov/pubmed/32809073
http://dx.doi.org/10.1186/s13568-020-01083-7
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