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Evaluation of the Immunosafety of Cucurbit[n]uril on Peripheral Blood Mononuclear Cells In Vitro

Cucurbiturils (CB[n]s) are nanoscale macrocyclic compounds capable of encapsulating a molecule or part of a molecule by forming host–guest complexes. Integration of drugs with CB[n] is used for the following purposes: controlling clearance; protection of the drug from biodegradation; targeted delive...

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Autores principales: Pashkina, Ekaterina, Aktanova, Alina, Blinova, Elena, Mirzaeva, Irina, Kovalenko, Ekaterina, Knauer, Nadezhda, Ermakov, Aleksandr, Kozlov, Vladimir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7435832/
https://www.ncbi.nlm.nih.gov/pubmed/32726898
http://dx.doi.org/10.3390/molecules25153388
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author Pashkina, Ekaterina
Aktanova, Alina
Blinova, Elena
Mirzaeva, Irina
Kovalenko, Ekaterina
Knauer, Nadezhda
Ermakov, Aleksandr
Kozlov, Vladimir
author_facet Pashkina, Ekaterina
Aktanova, Alina
Blinova, Elena
Mirzaeva, Irina
Kovalenko, Ekaterina
Knauer, Nadezhda
Ermakov, Aleksandr
Kozlov, Vladimir
author_sort Pashkina, Ekaterina
collection PubMed
description Cucurbiturils (CB[n]s) are nanoscale macrocyclic compounds capable of encapsulating a molecule or part of a molecule by forming host–guest complexes. Integration of drugs with CB[n] is used for the following purposes: controlling clearance; protection of the drug from biodegradation; targeted delivery to specific organs, tissues, or cells; reduction of toxicity; and improving solubility. One of the major problems encountered in the application of new drug delivery systems is lack of knowledge of their biological properties. CB[n], unlike many other often toxic nanoparticles, has extremely low toxicity, even at high doses. However, many aspects of the biological actions of these nanoscale cavitands remain unclear, including the immunotropic properties. In this study, we investigated the immunotoxicity and immunomodulation properties of CB[n]. It was found that CB[7] and CB[6] did not decrease the viability of mononuclear cells at all tested concentrations from 0.1–1 mM. Overall, the results indicated an immunomodulatory effect of different concentrations of CB[n]. In the case of a longer cultivation time, CB[n] had an immunostimulating effect, which was indicated by an enhancement of the proliferative activity of cells and increased expression of HLA-DR on lymphocytes.
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spelling pubmed-74358322020-08-25 Evaluation of the Immunosafety of Cucurbit[n]uril on Peripheral Blood Mononuclear Cells In Vitro Pashkina, Ekaterina Aktanova, Alina Blinova, Elena Mirzaeva, Irina Kovalenko, Ekaterina Knauer, Nadezhda Ermakov, Aleksandr Kozlov, Vladimir Molecules Article Cucurbiturils (CB[n]s) are nanoscale macrocyclic compounds capable of encapsulating a molecule or part of a molecule by forming host–guest complexes. Integration of drugs with CB[n] is used for the following purposes: controlling clearance; protection of the drug from biodegradation; targeted delivery to specific organs, tissues, or cells; reduction of toxicity; and improving solubility. One of the major problems encountered in the application of new drug delivery systems is lack of knowledge of their biological properties. CB[n], unlike many other often toxic nanoparticles, has extremely low toxicity, even at high doses. However, many aspects of the biological actions of these nanoscale cavitands remain unclear, including the immunotropic properties. In this study, we investigated the immunotoxicity and immunomodulation properties of CB[n]. It was found that CB[7] and CB[6] did not decrease the viability of mononuclear cells at all tested concentrations from 0.1–1 mM. Overall, the results indicated an immunomodulatory effect of different concentrations of CB[n]. In the case of a longer cultivation time, CB[n] had an immunostimulating effect, which was indicated by an enhancement of the proliferative activity of cells and increased expression of HLA-DR on lymphocytes. MDPI 2020-07-27 /pmc/articles/PMC7435832/ /pubmed/32726898 http://dx.doi.org/10.3390/molecules25153388 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pashkina, Ekaterina
Aktanova, Alina
Blinova, Elena
Mirzaeva, Irina
Kovalenko, Ekaterina
Knauer, Nadezhda
Ermakov, Aleksandr
Kozlov, Vladimir
Evaluation of the Immunosafety of Cucurbit[n]uril on Peripheral Blood Mononuclear Cells In Vitro
title Evaluation of the Immunosafety of Cucurbit[n]uril on Peripheral Blood Mononuclear Cells In Vitro
title_full Evaluation of the Immunosafety of Cucurbit[n]uril on Peripheral Blood Mononuclear Cells In Vitro
title_fullStr Evaluation of the Immunosafety of Cucurbit[n]uril on Peripheral Blood Mononuclear Cells In Vitro
title_full_unstemmed Evaluation of the Immunosafety of Cucurbit[n]uril on Peripheral Blood Mononuclear Cells In Vitro
title_short Evaluation of the Immunosafety of Cucurbit[n]uril on Peripheral Blood Mononuclear Cells In Vitro
title_sort evaluation of the immunosafety of cucurbit[n]uril on peripheral blood mononuclear cells in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7435832/
https://www.ncbi.nlm.nih.gov/pubmed/32726898
http://dx.doi.org/10.3390/molecules25153388
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