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Flavonoid and Non-Flavonoid Compounds of Autumn Royal and Egnatia Grape Skin Extracts Affect Membrane PUFA’s Profile and Cell Morphology in Human Colon Cancer Cell Lines

Grapes contain many flavonoid and non-flavonoid compounds with anticancer effects. In this work we fully characterized the polyphenolic profile of two grape skin extracts (GSEs), Autumn Royal and Egnatia, and assessed their effects on Polyunsaturated Fatty Acid (PUFA) membrane levels of Caco2 and SW...

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Autores principales: Tutino, Valeria, Gigante, Isabella, Milella, Rosa Anna, De Nunzio, Valentina, Flamini, Riccardo, De Rosso, Mirko, Scavo, Maria Principia, Depalo, Nicoletta, Fanizza, Elisabetta, Caruso, Maria Gabriella, Notarnicola, Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7435874/
https://www.ncbi.nlm.nih.gov/pubmed/32718061
http://dx.doi.org/10.3390/molecules25153352
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author Tutino, Valeria
Gigante, Isabella
Milella, Rosa Anna
De Nunzio, Valentina
Flamini, Riccardo
De Rosso, Mirko
Scavo, Maria Principia
Depalo, Nicoletta
Fanizza, Elisabetta
Caruso, Maria Gabriella
Notarnicola, Maria
author_facet Tutino, Valeria
Gigante, Isabella
Milella, Rosa Anna
De Nunzio, Valentina
Flamini, Riccardo
De Rosso, Mirko
Scavo, Maria Principia
Depalo, Nicoletta
Fanizza, Elisabetta
Caruso, Maria Gabriella
Notarnicola, Maria
author_sort Tutino, Valeria
collection PubMed
description Grapes contain many flavonoid and non-flavonoid compounds with anticancer effects. In this work we fully characterized the polyphenolic profile of two grape skin extracts (GSEs), Autumn Royal and Egnatia, and assessed their effects on Polyunsaturated Fatty Acid (PUFA) membrane levels of Caco2 and SW480 human colon cancer cell lines. Gene expression of 15-lipoxygenase-1 (15-LOX-1), and peroxisome proliferator-activated receptor gamma (PPAR-γ), as well as cell morphology, were evaluated. The polyphenolic composition was analyzed by Ultra-High-Performance Liquid Chromatography/Quadrupole-Time of Flight mass spectrometry (UHPLC/QTOF) analysis. PUFA levels were evaluated by gas chromatography, and gene expression levels of 15-LOX-1 and PPAR-γ were analyzed by real-time Polymerase Chain Reaction (PCR). Morphological cell changes caused by GSEs were identified by field emission scanning electron microscope (FE-SEM) and photomicrograph examination. We detected a different profile of flavonoid and non-flavonoid compounds in Autumn Royal and Egnatia GSEs. Cultured cells showed an increase of total PUFA levels mainly after treatment with Autumn Royal grape, and were richer in flavonoids when compared with the Egnatia variety. Both GSEs were able to affect 15-LOX-1 and PPAR-γ gene expression and cell morphology. Our results highlighted a new antitumor mechanism of GSEs that involves membrane PUFAs and their downstream pathways.
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spelling pubmed-74358742020-08-25 Flavonoid and Non-Flavonoid Compounds of Autumn Royal and Egnatia Grape Skin Extracts Affect Membrane PUFA’s Profile and Cell Morphology in Human Colon Cancer Cell Lines Tutino, Valeria Gigante, Isabella Milella, Rosa Anna De Nunzio, Valentina Flamini, Riccardo De Rosso, Mirko Scavo, Maria Principia Depalo, Nicoletta Fanizza, Elisabetta Caruso, Maria Gabriella Notarnicola, Maria Molecules Article Grapes contain many flavonoid and non-flavonoid compounds with anticancer effects. In this work we fully characterized the polyphenolic profile of two grape skin extracts (GSEs), Autumn Royal and Egnatia, and assessed their effects on Polyunsaturated Fatty Acid (PUFA) membrane levels of Caco2 and SW480 human colon cancer cell lines. Gene expression of 15-lipoxygenase-1 (15-LOX-1), and peroxisome proliferator-activated receptor gamma (PPAR-γ), as well as cell morphology, were evaluated. The polyphenolic composition was analyzed by Ultra-High-Performance Liquid Chromatography/Quadrupole-Time of Flight mass spectrometry (UHPLC/QTOF) analysis. PUFA levels were evaluated by gas chromatography, and gene expression levels of 15-LOX-1 and PPAR-γ were analyzed by real-time Polymerase Chain Reaction (PCR). Morphological cell changes caused by GSEs were identified by field emission scanning electron microscope (FE-SEM) and photomicrograph examination. We detected a different profile of flavonoid and non-flavonoid compounds in Autumn Royal and Egnatia GSEs. Cultured cells showed an increase of total PUFA levels mainly after treatment with Autumn Royal grape, and were richer in flavonoids when compared with the Egnatia variety. Both GSEs were able to affect 15-LOX-1 and PPAR-γ gene expression and cell morphology. Our results highlighted a new antitumor mechanism of GSEs that involves membrane PUFAs and their downstream pathways. MDPI 2020-07-23 /pmc/articles/PMC7435874/ /pubmed/32718061 http://dx.doi.org/10.3390/molecules25153352 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tutino, Valeria
Gigante, Isabella
Milella, Rosa Anna
De Nunzio, Valentina
Flamini, Riccardo
De Rosso, Mirko
Scavo, Maria Principia
Depalo, Nicoletta
Fanizza, Elisabetta
Caruso, Maria Gabriella
Notarnicola, Maria
Flavonoid and Non-Flavonoid Compounds of Autumn Royal and Egnatia Grape Skin Extracts Affect Membrane PUFA’s Profile and Cell Morphology in Human Colon Cancer Cell Lines
title Flavonoid and Non-Flavonoid Compounds of Autumn Royal and Egnatia Grape Skin Extracts Affect Membrane PUFA’s Profile and Cell Morphology in Human Colon Cancer Cell Lines
title_full Flavonoid and Non-Flavonoid Compounds of Autumn Royal and Egnatia Grape Skin Extracts Affect Membrane PUFA’s Profile and Cell Morphology in Human Colon Cancer Cell Lines
title_fullStr Flavonoid and Non-Flavonoid Compounds of Autumn Royal and Egnatia Grape Skin Extracts Affect Membrane PUFA’s Profile and Cell Morphology in Human Colon Cancer Cell Lines
title_full_unstemmed Flavonoid and Non-Flavonoid Compounds of Autumn Royal and Egnatia Grape Skin Extracts Affect Membrane PUFA’s Profile and Cell Morphology in Human Colon Cancer Cell Lines
title_short Flavonoid and Non-Flavonoid Compounds of Autumn Royal and Egnatia Grape Skin Extracts Affect Membrane PUFA’s Profile and Cell Morphology in Human Colon Cancer Cell Lines
title_sort flavonoid and non-flavonoid compounds of autumn royal and egnatia grape skin extracts affect membrane pufa’s profile and cell morphology in human colon cancer cell lines
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7435874/
https://www.ncbi.nlm.nih.gov/pubmed/32718061
http://dx.doi.org/10.3390/molecules25153352
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