Leachable Poly(Trimethylene Carbonate)/CaCO(3) Composites for Additive Manufacturing of Microporous Vascular Structures

The aim of this work was to fabricate microporous poly(trimethylene carbonate) (PTMC) vascular structures by stereolithography (SLA) for applications in tissue engineering and organ models. Leachable CaCO(3) particles with an average size of 0.56 μm were used as porogens. Composites of photocrosslinkable PTMC and CaCO(3) particles were cast on glass plates, crosslinked by ultraviolet light treatment and leached in watery HCl solutions. In order to obtain interconnected pore structures, the PTMC/CaCO(3) composites had to contain at least 30 vol % CaCO(3). Leached PTMC films had porosities ranging from 33% to 71% and a pore size of around 0.5 μm. The mechanical properties of the microporous PTMC films matched with those of natural blood vessels. Resins based on PTMC/CaCO(3) composites with 45 vol % CaCO(3) particles were formulated and successfully used to build vascular structures of various shapes and sizes by SLA. The intrinsic permeabilities of the microporous PTMC films and vascular structures were at least one order of magnitude higher than reported for the extracellular matrix, indicating no mass transfer limitations in the case of cell seeding.