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Phytosynthesis of Palladium Nanoclusters: An Efficient Nanozyme for Ultrasensitive and Selective Detection of Reactive Oxygen Species
Hydrogen peroxide is a low-reactivity reactive oxygen species (ROS); however, it can easily penetrate cell membranes and produce highly reactive hydroxyl radical species through Fenton’s reaction. Its presence in abnormal amounts can lead to serious diseases in humans. Although the development of a...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7436022/ https://www.ncbi.nlm.nih.gov/pubmed/32717976 http://dx.doi.org/10.3390/molecules25153349 |
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author | Tripathi, Ravi Mani Chung, Sang J. |
author_facet | Tripathi, Ravi Mani Chung, Sang J. |
author_sort | Tripathi, Ravi Mani |
collection | PubMed |
description | Hydrogen peroxide is a low-reactivity reactive oxygen species (ROS); however, it can easily penetrate cell membranes and produce highly reactive hydroxyl radical species through Fenton’s reaction. Its presence in abnormal amounts can lead to serious diseases in humans. Although the development of a simple, ultrasensitive, and selective method for H(2)O(2) detection is crucial, this remains a strategic challenge. The peroxidase mimetic activity of palladium nanoclusters (PdNCs) has not previously been evaluated. In this study, we developed an ultrasensitive and selective colorimetric detection method for H(2)O(2) using PdNCs. An unprecedented eco-friendly, cost-effective, and facile biological method was developed for the synthesis of PdNCs. This is the first report of the biosynthesis of PdNCs. The synthesized nanoclusters had a significantly narrow size distribution profile and high stability. The nanoclusters were demonstrated to possess a peroxidase mimetic activity that could oxidize peroxidase substrate 3,3′,5,5′-tetramethylbenzidine (TMB). Various interfering substances in serum (100 μM phenylalanine, cysteine, tryptophan, arginine, glucose, urea, Na(+), Fe(2+), PO(4)(3−), Mn(+2), Ca(2+), Mg(2+), Zn(2+), NH(4)(+), and K(+)) were included to evaluate the selectivity of the assay, and oxidation of TMB occurred only in the presence of H(2)O(2). Therefore, PdNCs show an efficient nanozyme for the peroxidase mimetic activity. The assay produced a sufficient signal at the ultralow concentration of 0.0625 µM H(2)O(2). This colorimetric assay provides a real-time, rapid, and easy-to-use platform for the detection of H(2)O(2) for clinical purposes. |
format | Online Article Text |
id | pubmed-7436022 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-74360222020-08-24 Phytosynthesis of Palladium Nanoclusters: An Efficient Nanozyme for Ultrasensitive and Selective Detection of Reactive Oxygen Species Tripathi, Ravi Mani Chung, Sang J. Molecules Article Hydrogen peroxide is a low-reactivity reactive oxygen species (ROS); however, it can easily penetrate cell membranes and produce highly reactive hydroxyl radical species through Fenton’s reaction. Its presence in abnormal amounts can lead to serious diseases in humans. Although the development of a simple, ultrasensitive, and selective method for H(2)O(2) detection is crucial, this remains a strategic challenge. The peroxidase mimetic activity of palladium nanoclusters (PdNCs) has not previously been evaluated. In this study, we developed an ultrasensitive and selective colorimetric detection method for H(2)O(2) using PdNCs. An unprecedented eco-friendly, cost-effective, and facile biological method was developed for the synthesis of PdNCs. This is the first report of the biosynthesis of PdNCs. The synthesized nanoclusters had a significantly narrow size distribution profile and high stability. The nanoclusters were demonstrated to possess a peroxidase mimetic activity that could oxidize peroxidase substrate 3,3′,5,5′-tetramethylbenzidine (TMB). Various interfering substances in serum (100 μM phenylalanine, cysteine, tryptophan, arginine, glucose, urea, Na(+), Fe(2+), PO(4)(3−), Mn(+2), Ca(2+), Mg(2+), Zn(2+), NH(4)(+), and K(+)) were included to evaluate the selectivity of the assay, and oxidation of TMB occurred only in the presence of H(2)O(2). Therefore, PdNCs show an efficient nanozyme for the peroxidase mimetic activity. The assay produced a sufficient signal at the ultralow concentration of 0.0625 µM H(2)O(2). This colorimetric assay provides a real-time, rapid, and easy-to-use platform for the detection of H(2)O(2) for clinical purposes. MDPI 2020-07-23 /pmc/articles/PMC7436022/ /pubmed/32717976 http://dx.doi.org/10.3390/molecules25153349 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Tripathi, Ravi Mani Chung, Sang J. Phytosynthesis of Palladium Nanoclusters: An Efficient Nanozyme for Ultrasensitive and Selective Detection of Reactive Oxygen Species |
title | Phytosynthesis of Palladium Nanoclusters: An Efficient Nanozyme for Ultrasensitive and Selective Detection of Reactive Oxygen Species |
title_full | Phytosynthesis of Palladium Nanoclusters: An Efficient Nanozyme for Ultrasensitive and Selective Detection of Reactive Oxygen Species |
title_fullStr | Phytosynthesis of Palladium Nanoclusters: An Efficient Nanozyme for Ultrasensitive and Selective Detection of Reactive Oxygen Species |
title_full_unstemmed | Phytosynthesis of Palladium Nanoclusters: An Efficient Nanozyme for Ultrasensitive and Selective Detection of Reactive Oxygen Species |
title_short | Phytosynthesis of Palladium Nanoclusters: An Efficient Nanozyme for Ultrasensitive and Selective Detection of Reactive Oxygen Species |
title_sort | phytosynthesis of palladium nanoclusters: an efficient nanozyme for ultrasensitive and selective detection of reactive oxygen species |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7436022/ https://www.ncbi.nlm.nih.gov/pubmed/32717976 http://dx.doi.org/10.3390/molecules25153349 |
work_keys_str_mv | AT tripathiravimani phytosynthesisofpalladiumnanoclustersanefficientnanozymeforultrasensitiveandselectivedetectionofreactiveoxygenspecies AT chungsangj phytosynthesisofpalladiumnanoclustersanefficientnanozymeforultrasensitiveandselectivedetectionofreactiveoxygenspecies |