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Micheliolide suppresses the viability, migration and invasion of U251MG cells via the NF-κB signaling pathway

Micheliolide (MCL), a sesquiterpene lactone isolated from Michelia compressa and Michelia champaca, has been used previously to inhibit the NF-κB signaling pathway. MCL has exerted various therapeutic effects in numerous types of disease, such as inflammatory and cancer. However, to the best of our...

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Autores principales: Feng, Dingkun, Liu, Min, Liu, Yanting, Zhao, Xiaojin, Sun, Huan, Zheng, Xu, Zhu, Jiabin, Shang, Fajun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7436293/
https://www.ncbi.nlm.nih.gov/pubmed/32863900
http://dx.doi.org/10.3892/ol.2020.11928
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author Feng, Dingkun
Liu, Min
Liu, Yanting
Zhao, Xiaojin
Sun, Huan
Zheng, Xu
Zhu, Jiabin
Shang, Fajun
author_facet Feng, Dingkun
Liu, Min
Liu, Yanting
Zhao, Xiaojin
Sun, Huan
Zheng, Xu
Zhu, Jiabin
Shang, Fajun
author_sort Feng, Dingkun
collection PubMed
description Micheliolide (MCL), a sesquiterpene lactone isolated from Michelia compressa and Michelia champaca, has been used previously to inhibit the NF-κB signaling pathway. MCL has exerted various therapeutic effects in numerous types of disease, such as inflammatory and cancer. However, to the best of our knowledge, its underlying anticancer mechanism remains to be understood. The present study aimed to investigate the effects of MCL on human glioma U251MG cells and to determine the potential anticancer mechanism of action of MCL. From Cell Counting Kit-8, colony formation assay, apoptosis assay and Confocal immunofluorescence imaging analysis, the results revealed that MCL significantly inhibited cell viability in vitro and induced cell apoptosis via activation of the cytochrome c/caspase-dependent apoptotic pathway. In addition, MCL also suppressed cell invasion and metastasis via the wound healing and Transwell invasion assays. Furthermore, western blot and reverse transcription PCR analyses demonstrated that MCL significantly downregulated cyclooxygenase-2 (COX-2) expression levels, which may have partially occurred through the inactivation of the NF-κB signaling pathway. In conclusion, the results of the present study indicated that MCL may inhibit glioma carcinoma growth by downregulating the NF-κB/COX-2 signaling pathway, which suggested that MCL may be a novel and alternative antitumor agent for the treatment of human glioma carcinoma.
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spelling pubmed-74362932020-08-27 Micheliolide suppresses the viability, migration and invasion of U251MG cells via the NF-κB signaling pathway Feng, Dingkun Liu, Min Liu, Yanting Zhao, Xiaojin Sun, Huan Zheng, Xu Zhu, Jiabin Shang, Fajun Oncol Lett Articles Micheliolide (MCL), a sesquiterpene lactone isolated from Michelia compressa and Michelia champaca, has been used previously to inhibit the NF-κB signaling pathway. MCL has exerted various therapeutic effects in numerous types of disease, such as inflammatory and cancer. However, to the best of our knowledge, its underlying anticancer mechanism remains to be understood. The present study aimed to investigate the effects of MCL on human glioma U251MG cells and to determine the potential anticancer mechanism of action of MCL. From Cell Counting Kit-8, colony formation assay, apoptosis assay and Confocal immunofluorescence imaging analysis, the results revealed that MCL significantly inhibited cell viability in vitro and induced cell apoptosis via activation of the cytochrome c/caspase-dependent apoptotic pathway. In addition, MCL also suppressed cell invasion and metastasis via the wound healing and Transwell invasion assays. Furthermore, western blot and reverse transcription PCR analyses demonstrated that MCL significantly downregulated cyclooxygenase-2 (COX-2) expression levels, which may have partially occurred through the inactivation of the NF-κB signaling pathway. In conclusion, the results of the present study indicated that MCL may inhibit glioma carcinoma growth by downregulating the NF-κB/COX-2 signaling pathway, which suggested that MCL may be a novel and alternative antitumor agent for the treatment of human glioma carcinoma. D.A. Spandidos 2020-10 2020-07-29 /pmc/articles/PMC7436293/ /pubmed/32863900 http://dx.doi.org/10.3892/ol.2020.11928 Text en Copyright: © Feng et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Feng, Dingkun
Liu, Min
Liu, Yanting
Zhao, Xiaojin
Sun, Huan
Zheng, Xu
Zhu, Jiabin
Shang, Fajun
Micheliolide suppresses the viability, migration and invasion of U251MG cells via the NF-κB signaling pathway
title Micheliolide suppresses the viability, migration and invasion of U251MG cells via the NF-κB signaling pathway
title_full Micheliolide suppresses the viability, migration and invasion of U251MG cells via the NF-κB signaling pathway
title_fullStr Micheliolide suppresses the viability, migration and invasion of U251MG cells via the NF-κB signaling pathway
title_full_unstemmed Micheliolide suppresses the viability, migration and invasion of U251MG cells via the NF-κB signaling pathway
title_short Micheliolide suppresses the viability, migration and invasion of U251MG cells via the NF-κB signaling pathway
title_sort micheliolide suppresses the viability, migration and invasion of u251mg cells via the nf-κb signaling pathway
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7436293/
https://www.ncbi.nlm.nih.gov/pubmed/32863900
http://dx.doi.org/10.3892/ol.2020.11928
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