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Reduced graphene oxide–based field effect transistors for the detection of E7 protein of human papillomavirus in saliva
Several challenging biological sensing concepts have been realized using electrolyte-gated reduced graphene oxide field effect transistors (rGO-FETs). In this work, we demonstrate the interest of rGO-FET for the sensing of human papillomavirus (HPV), one of the most common sexually transmitted virus...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7438402/ https://www.ncbi.nlm.nih.gov/pubmed/32816088 http://dx.doi.org/10.1007/s00216-020-02879-z |
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author | Aspermair, Patrik Mishyn, Vladyslav Bintinger, Johannes Happy, Henri Bagga, Komal Subramanian, Palaniappan Knoll, Wolfgang Boukherroub, Rabah Szunerits, Sabine |
author_facet | Aspermair, Patrik Mishyn, Vladyslav Bintinger, Johannes Happy, Henri Bagga, Komal Subramanian, Palaniappan Knoll, Wolfgang Boukherroub, Rabah Szunerits, Sabine |
author_sort | Aspermair, Patrik |
collection | PubMed |
description | Several challenging biological sensing concepts have been realized using electrolyte-gated reduced graphene oxide field effect transistors (rGO-FETs). In this work, we demonstrate the interest of rGO-FET for the sensing of human papillomavirus (HPV), one of the most common sexually transmitted viruses and a necessary factor for cervical carcinogenesis. The highly sensitive and selective detection of the HPV-16 E7 protein relies on the attractive semiconducting characteristics of pyrene-modified rGO functionalized with RNA aptamer Sc5-c3. The aptamer-functionalized rGO-FET allows for monitoring the aptamer-HPV-16 E7 protein binding in real time with a detection limit of about 100 pg mL(−1) (1.75 nM) for HPV-16 E7 from five blank noise signals (95% confidence level). The feasibility of this method for clinical application in point-of-care technology is evaluated using HPV-16 E7 protein suspended in saliva and demonstrates the successful fabrication of a promising field effect transistor biosensor for HPV diagnosis. Graphical abstract [Image: see text] |
format | Online Article Text |
id | pubmed-7438402 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-74384022020-08-20 Reduced graphene oxide–based field effect transistors for the detection of E7 protein of human papillomavirus in saliva Aspermair, Patrik Mishyn, Vladyslav Bintinger, Johannes Happy, Henri Bagga, Komal Subramanian, Palaniappan Knoll, Wolfgang Boukherroub, Rabah Szunerits, Sabine Anal Bioanal Chem Research Paper Several challenging biological sensing concepts have been realized using electrolyte-gated reduced graphene oxide field effect transistors (rGO-FETs). In this work, we demonstrate the interest of rGO-FET for the sensing of human papillomavirus (HPV), one of the most common sexually transmitted viruses and a necessary factor for cervical carcinogenesis. The highly sensitive and selective detection of the HPV-16 E7 protein relies on the attractive semiconducting characteristics of pyrene-modified rGO functionalized with RNA aptamer Sc5-c3. The aptamer-functionalized rGO-FET allows for monitoring the aptamer-HPV-16 E7 protein binding in real time with a detection limit of about 100 pg mL(−1) (1.75 nM) for HPV-16 E7 from five blank noise signals (95% confidence level). The feasibility of this method for clinical application in point-of-care technology is evaluated using HPV-16 E7 protein suspended in saliva and demonstrates the successful fabrication of a promising field effect transistor biosensor for HPV diagnosis. Graphical abstract [Image: see text] Springer Berlin Heidelberg 2020-08-20 2021 /pmc/articles/PMC7438402/ /pubmed/32816088 http://dx.doi.org/10.1007/s00216-020-02879-z Text en © Springer-Verlag GmbH Germany, part of Springer Nature 2020 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Research Paper Aspermair, Patrik Mishyn, Vladyslav Bintinger, Johannes Happy, Henri Bagga, Komal Subramanian, Palaniappan Knoll, Wolfgang Boukherroub, Rabah Szunerits, Sabine Reduced graphene oxide–based field effect transistors for the detection of E7 protein of human papillomavirus in saliva |
title | Reduced graphene oxide–based field effect transistors for the detection of E7 protein of human papillomavirus in saliva |
title_full | Reduced graphene oxide–based field effect transistors for the detection of E7 protein of human papillomavirus in saliva |
title_fullStr | Reduced graphene oxide–based field effect transistors for the detection of E7 protein of human papillomavirus in saliva |
title_full_unstemmed | Reduced graphene oxide–based field effect transistors for the detection of E7 protein of human papillomavirus in saliva |
title_short | Reduced graphene oxide–based field effect transistors for the detection of E7 protein of human papillomavirus in saliva |
title_sort | reduced graphene oxide–based field effect transistors for the detection of e7 protein of human papillomavirus in saliva |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7438402/ https://www.ncbi.nlm.nih.gov/pubmed/32816088 http://dx.doi.org/10.1007/s00216-020-02879-z |
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