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Conventional Dendritic Cells and Slan(+) Monocytes During HIV-2 Infection

HIV-2 infection is characterized by low viremia and slow disease progression as compared to HIV-1 infection. Circulating CD14(++)CD16(+) monocytes were found to accumulate and CD11c(+) conventional dendritic cells (cDC) to be depleted in a Portuguese cohort of people living with HIV-2 (PLWHIV-2), co...

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Autores principales: Iannetta, Marco, Isnard, Stéphane, Manuzak, Jennifer, Guillerme, Jean-Baptiste, Notin, Mathilde, Bailly, Karine, Andrieu, Muriel, Amraoui, Sonia, Vimeux, Lene, Figueiredo, Suzanne, Charmeteau-de Muylder, Bénédicte, Vaton, Laura, Hatton, Etienne X., Samri, Assia, Autran, Brigitte, Thiébaut, Rodolphe, Chaghil, Nathalie, Glohi, David, Charpentier, Charlotte, Descamps, Diane, Brun-Vézinet, Françoise, Matheron, Sophie, Cheynier, Remi, Hosmalin, Anne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7438582/
https://www.ncbi.nlm.nih.gov/pubmed/32903610
http://dx.doi.org/10.3389/fimmu.2020.01658
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author Iannetta, Marco
Isnard, Stéphane
Manuzak, Jennifer
Guillerme, Jean-Baptiste
Notin, Mathilde
Bailly, Karine
Andrieu, Muriel
Amraoui, Sonia
Vimeux, Lene
Figueiredo, Suzanne
Charmeteau-de Muylder, Bénédicte
Vaton, Laura
Hatton, Etienne X.
Samri, Assia
Autran, Brigitte
Thiébaut, Rodolphe
Chaghil, Nathalie
Glohi, David
Charpentier, Charlotte
Descamps, Diane
Brun-Vézinet, Françoise
Matheron, Sophie
Cheynier, Remi
Hosmalin, Anne
author_facet Iannetta, Marco
Isnard, Stéphane
Manuzak, Jennifer
Guillerme, Jean-Baptiste
Notin, Mathilde
Bailly, Karine
Andrieu, Muriel
Amraoui, Sonia
Vimeux, Lene
Figueiredo, Suzanne
Charmeteau-de Muylder, Bénédicte
Vaton, Laura
Hatton, Etienne X.
Samri, Assia
Autran, Brigitte
Thiébaut, Rodolphe
Chaghil, Nathalie
Glohi, David
Charpentier, Charlotte
Descamps, Diane
Brun-Vézinet, Françoise
Matheron, Sophie
Cheynier, Remi
Hosmalin, Anne
author_sort Iannetta, Marco
collection PubMed
description HIV-2 infection is characterized by low viremia and slow disease progression as compared to HIV-1 infection. Circulating CD14(++)CD16(+) monocytes were found to accumulate and CD11c(+) conventional dendritic cells (cDC) to be depleted in a Portuguese cohort of people living with HIV-2 (PLWHIV-2), compared to blood bank healthy donors (HD). We studied more precisely classical monocytes; CD16(+) inflammatory (intermediate, non-classical and slan(+) monocytes, known to accumulate during viremic HIV-1 infection); cDC1, important for cross-presentation, and cDC2, both depleted during HIV-1 infection. We analyzed by flow cytometry these PBMC subsets from Paris area residents: 29 asymptomatic, untreated PLWHIV-2 from the IMMUNOVIR-2 study, part of the ANRS-CO5 HIV-2 cohort: 19 long-term non-progressors (LTNP; infection ≥8 years, undetectable viral load, stable CD4 counts≥500/μL; 17 of West-African origin -WA), and 10 non-LTNP (P; progressive infection; 9 WA); and 30 age-and sex-matched controls: 16 blood bank HD with unknown geographical origin, and 10 HD of WA origin (GeoHD). We measured plasma bacterial translocation markers by ELISA. Non-classical monocyte counts were higher in GeoHD than in HD (54 vs. 32 cells/μL, p = 0.0002). Slan(+) monocyte counts were twice as high in GeoHD than in HD (WA: 28 vs. 13 cells/μL, p = 0.0002). Thus cell counts were compared only between participants of WA origin. They were similar in LTNP, P and GeoHD, indicating that there were no HIV-2 related differences. cDC counts did not show major differences between the groups. Interestingly, inflammatory monocyte counts correlated with plasma sCD14 and LBP only in PLWHIV-2, especially LTNP, and not in GeoHD. In conclusion, in LTNP PLWHIV-2, inflammatory monocyte counts correlated with LBP or sCD14 plasma levels, indicating a potential innate immune response to subclinical bacterial translocation. As GeoHD had higher inflammatory monocyte counts than HD, our data also show that specific controls are important to refine innate immunity studies.
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spelling pubmed-74385822020-09-03 Conventional Dendritic Cells and Slan(+) Monocytes During HIV-2 Infection Iannetta, Marco Isnard, Stéphane Manuzak, Jennifer Guillerme, Jean-Baptiste Notin, Mathilde Bailly, Karine Andrieu, Muriel Amraoui, Sonia Vimeux, Lene Figueiredo, Suzanne Charmeteau-de Muylder, Bénédicte Vaton, Laura Hatton, Etienne X. Samri, Assia Autran, Brigitte Thiébaut, Rodolphe Chaghil, Nathalie Glohi, David Charpentier, Charlotte Descamps, Diane Brun-Vézinet, Françoise Matheron, Sophie Cheynier, Remi Hosmalin, Anne Front Immunol Immunology HIV-2 infection is characterized by low viremia and slow disease progression as compared to HIV-1 infection. Circulating CD14(++)CD16(+) monocytes were found to accumulate and CD11c(+) conventional dendritic cells (cDC) to be depleted in a Portuguese cohort of people living with HIV-2 (PLWHIV-2), compared to blood bank healthy donors (HD). We studied more precisely classical monocytes; CD16(+) inflammatory (intermediate, non-classical and slan(+) monocytes, known to accumulate during viremic HIV-1 infection); cDC1, important for cross-presentation, and cDC2, both depleted during HIV-1 infection. We analyzed by flow cytometry these PBMC subsets from Paris area residents: 29 asymptomatic, untreated PLWHIV-2 from the IMMUNOVIR-2 study, part of the ANRS-CO5 HIV-2 cohort: 19 long-term non-progressors (LTNP; infection ≥8 years, undetectable viral load, stable CD4 counts≥500/μL; 17 of West-African origin -WA), and 10 non-LTNP (P; progressive infection; 9 WA); and 30 age-and sex-matched controls: 16 blood bank HD with unknown geographical origin, and 10 HD of WA origin (GeoHD). We measured plasma bacterial translocation markers by ELISA. Non-classical monocyte counts were higher in GeoHD than in HD (54 vs. 32 cells/μL, p = 0.0002). Slan(+) monocyte counts were twice as high in GeoHD than in HD (WA: 28 vs. 13 cells/μL, p = 0.0002). Thus cell counts were compared only between participants of WA origin. They were similar in LTNP, P and GeoHD, indicating that there were no HIV-2 related differences. cDC counts did not show major differences between the groups. Interestingly, inflammatory monocyte counts correlated with plasma sCD14 and LBP only in PLWHIV-2, especially LTNP, and not in GeoHD. In conclusion, in LTNP PLWHIV-2, inflammatory monocyte counts correlated with LBP or sCD14 plasma levels, indicating a potential innate immune response to subclinical bacterial translocation. As GeoHD had higher inflammatory monocyte counts than HD, our data also show that specific controls are important to refine innate immunity studies. Frontiers Media S.A. 2020-08-13 /pmc/articles/PMC7438582/ /pubmed/32903610 http://dx.doi.org/10.3389/fimmu.2020.01658 Text en Copyright © 2020 Iannetta, Isnard, Manuzak, Guillerme, Notin, Bailly, Andrieu, Amraoui, Vimeux, Figueiredo, Charmeteau-de Muylder, Vaton, Hatton, Samri, Autran, Thiébaut, Chaghil, Glohi, Charpentier, Descamps, Brun-Vézinet, Matheron, Cheynier and Hosmalin. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Iannetta, Marco
Isnard, Stéphane
Manuzak, Jennifer
Guillerme, Jean-Baptiste
Notin, Mathilde
Bailly, Karine
Andrieu, Muriel
Amraoui, Sonia
Vimeux, Lene
Figueiredo, Suzanne
Charmeteau-de Muylder, Bénédicte
Vaton, Laura
Hatton, Etienne X.
Samri, Assia
Autran, Brigitte
Thiébaut, Rodolphe
Chaghil, Nathalie
Glohi, David
Charpentier, Charlotte
Descamps, Diane
Brun-Vézinet, Françoise
Matheron, Sophie
Cheynier, Remi
Hosmalin, Anne
Conventional Dendritic Cells and Slan(+) Monocytes During HIV-2 Infection
title Conventional Dendritic Cells and Slan(+) Monocytes During HIV-2 Infection
title_full Conventional Dendritic Cells and Slan(+) Monocytes During HIV-2 Infection
title_fullStr Conventional Dendritic Cells and Slan(+) Monocytes During HIV-2 Infection
title_full_unstemmed Conventional Dendritic Cells and Slan(+) Monocytes During HIV-2 Infection
title_short Conventional Dendritic Cells and Slan(+) Monocytes During HIV-2 Infection
title_sort conventional dendritic cells and slan(+) monocytes during hiv-2 infection
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7438582/
https://www.ncbi.nlm.nih.gov/pubmed/32903610
http://dx.doi.org/10.3389/fimmu.2020.01658
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