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Surface Triggered Self-Assembly of Fmoc-Tripeptide as an Antibacterial Coating
In western countries, one patient on twenty will develop a nosocomial infection during his hospitalization at health care facilities. Classical antibiotics being less and less effective, this phenomenon is expanding year after year. Prevention of bacteria colonization of implantable medical devices...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7438842/ https://www.ncbi.nlm.nih.gov/pubmed/32974302 http://dx.doi.org/10.3389/fbioe.2020.00938 |
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author | Criado-Gonzalez, Miryam Iqbal, Muhammad Haseeb Carvalho, Alain Schmutz, Marc Jierry, Loïc Schaaf, Pierre Boulmedais, Fouzia |
author_facet | Criado-Gonzalez, Miryam Iqbal, Muhammad Haseeb Carvalho, Alain Schmutz, Marc Jierry, Loïc Schaaf, Pierre Boulmedais, Fouzia |
author_sort | Criado-Gonzalez, Miryam |
collection | PubMed |
description | In western countries, one patient on twenty will develop a nosocomial infection during his hospitalization at health care facilities. Classical antibiotics being less and less effective, this phenomenon is expanding year after year. Prevention of bacteria colonization of implantable medical devices constitutes a major medical and financial issue. In this study, we developed an antibacterial coating based on self-assembled Fmoc-tripeptide. Fmoc-FFpY peptides (F: phenylalanine; Y: tyrosine; p: PO(4)(2–)) are dephosphorylated enzymatically into Fmoc-FFY by action of alkaline phosphatase functionalized silica nanoparticles (NPs@AP), previously deposited on a surface. Fmoc-FFY peptides then self-assemble through π–π stacking interactions, hydrogen bonds and hydrophobic interactions adopting β-sheets secondary structures. The obtained hydrogel coatings show fibrillary structures observed by cryo-scanning electron microscopy with a thickness of few micrometers. At low concentration (≤0.5 mg.mL(–1)), self-assembled Fmoc-FFY has a superior antibacterial activity than Fmoc-FFpY peptide in solution. After 24 h of incubation, Fmoc-FFY hydrogel coatings fully inhibit the development of Gram-positive Staphylococcus aureus (S. aureus). The antibacterial effect is maintained on an in vitro model of repetitive infection in the case of S. aureus. This coating could serve in infections were Gram positive bacteria are prevalent, e.g., intravascular catheter infections. This work gives new insights toward the design of an alternative antimicrobial coating. |
format | Online Article Text |
id | pubmed-7438842 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-74388422020-09-23 Surface Triggered Self-Assembly of Fmoc-Tripeptide as an Antibacterial Coating Criado-Gonzalez, Miryam Iqbal, Muhammad Haseeb Carvalho, Alain Schmutz, Marc Jierry, Loïc Schaaf, Pierre Boulmedais, Fouzia Front Bioeng Biotechnol Bioengineering and Biotechnology In western countries, one patient on twenty will develop a nosocomial infection during his hospitalization at health care facilities. Classical antibiotics being less and less effective, this phenomenon is expanding year after year. Prevention of bacteria colonization of implantable medical devices constitutes a major medical and financial issue. In this study, we developed an antibacterial coating based on self-assembled Fmoc-tripeptide. Fmoc-FFpY peptides (F: phenylalanine; Y: tyrosine; p: PO(4)(2–)) are dephosphorylated enzymatically into Fmoc-FFY by action of alkaline phosphatase functionalized silica nanoparticles (NPs@AP), previously deposited on a surface. Fmoc-FFY peptides then self-assemble through π–π stacking interactions, hydrogen bonds and hydrophobic interactions adopting β-sheets secondary structures. The obtained hydrogel coatings show fibrillary structures observed by cryo-scanning electron microscopy with a thickness of few micrometers. At low concentration (≤0.5 mg.mL(–1)), self-assembled Fmoc-FFY has a superior antibacterial activity than Fmoc-FFpY peptide in solution. After 24 h of incubation, Fmoc-FFY hydrogel coatings fully inhibit the development of Gram-positive Staphylococcus aureus (S. aureus). The antibacterial effect is maintained on an in vitro model of repetitive infection in the case of S. aureus. This coating could serve in infections were Gram positive bacteria are prevalent, e.g., intravascular catheter infections. This work gives new insights toward the design of an alternative antimicrobial coating. Frontiers Media S.A. 2020-08-07 /pmc/articles/PMC7438842/ /pubmed/32974302 http://dx.doi.org/10.3389/fbioe.2020.00938 Text en Copyright © 2020 Criado-Gonzalez, Iqbal, Carvalho, Schmutz, Jierry, Schaaf and Boulmedais. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Criado-Gonzalez, Miryam Iqbal, Muhammad Haseeb Carvalho, Alain Schmutz, Marc Jierry, Loïc Schaaf, Pierre Boulmedais, Fouzia Surface Triggered Self-Assembly of Fmoc-Tripeptide as an Antibacterial Coating |
title | Surface Triggered Self-Assembly of Fmoc-Tripeptide as an Antibacterial Coating |
title_full | Surface Triggered Self-Assembly of Fmoc-Tripeptide as an Antibacterial Coating |
title_fullStr | Surface Triggered Self-Assembly of Fmoc-Tripeptide as an Antibacterial Coating |
title_full_unstemmed | Surface Triggered Self-Assembly of Fmoc-Tripeptide as an Antibacterial Coating |
title_short | Surface Triggered Self-Assembly of Fmoc-Tripeptide as an Antibacterial Coating |
title_sort | surface triggered self-assembly of fmoc-tripeptide as an antibacterial coating |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7438842/ https://www.ncbi.nlm.nih.gov/pubmed/32974302 http://dx.doi.org/10.3389/fbioe.2020.00938 |
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