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Analysis of Resistance to Florfenicol and the Related Mechanism of Dissemination in Different Animal-Derived Bacteria

Bacterial resistance to antibiotics has become an important concern for public health. This study was aimed to investigate the characteristics and the distribution of the florfenicol-related resistance genes in bacteria isolated from four farms. A total of 106 florfenicol-resistant Gram-negative bac...

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Autores principales: Li, Peizhen, Zhu, Tingyuan, Zhou, Danying, Lu, Wei, Liu, Hongmao, Sun, Zhewei, Ying, Jun, Lu, Junwan, Lin, Xi, Li, Kewei, Ying, Jianchao, Bao, Qiyu, Xu, Teng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7438884/
https://www.ncbi.nlm.nih.gov/pubmed/32903722
http://dx.doi.org/10.3389/fcimb.2020.00369
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author Li, Peizhen
Zhu, Tingyuan
Zhou, Danying
Lu, Wei
Liu, Hongmao
Sun, Zhewei
Ying, Jun
Lu, Junwan
Lin, Xi
Li, Kewei
Ying, Jianchao
Bao, Qiyu
Xu, Teng
author_facet Li, Peizhen
Zhu, Tingyuan
Zhou, Danying
Lu, Wei
Liu, Hongmao
Sun, Zhewei
Ying, Jun
Lu, Junwan
Lin, Xi
Li, Kewei
Ying, Jianchao
Bao, Qiyu
Xu, Teng
author_sort Li, Peizhen
collection PubMed
description Bacterial resistance to antibiotics has become an important concern for public health. This study was aimed to investigate the characteristics and the distribution of the florfenicol-related resistance genes in bacteria isolated from four farms. A total of 106 florfenicol-resistant Gram-negative bacilli were examined for florfenicol-related resistance genes, and the positive isolates were further characterized. The antimicrobial sensitivity results showed that most of them (100, 94.33%) belonged to multidrug resistance Enterobacteriaceae. About 91.51% of the strains carried floR gene, while 4.72% carried cfr gene. According to the pulsed-field gel electrophoresis results, 34 Escherichia coli were subdivided into 22 profiles, the genetic similarity coefficient of which ranged from 80.3 to 98.0%. The multilocus sequence typing (MLST) results revealed 17 sequence types (STs), with ST10 being the most prevalent. The genome sequencing result showed that the Proteus vulgaris G32 genome consists of a 4.06-Mb chromosome, a 177,911-bp plasmid (pG32-177), and a 51,686-bp plasmid (pG32-51). A floR located in a drug-resistant region on the chromosome of P. vulgaris G32 was with IS91 family transposase, and the other floR gene on the plasmid pG32-177 was with an ISCR2 insertion sequence. The cfr gene was located on the pG32-51 flanked by IS26 element and TnpA26. This study suggested that the mobile genetic elements played an important role in the replication of resistance genes and the horizontal resistance gene transfer.
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spelling pubmed-74388842020-09-03 Analysis of Resistance to Florfenicol and the Related Mechanism of Dissemination in Different Animal-Derived Bacteria Li, Peizhen Zhu, Tingyuan Zhou, Danying Lu, Wei Liu, Hongmao Sun, Zhewei Ying, Jun Lu, Junwan Lin, Xi Li, Kewei Ying, Jianchao Bao, Qiyu Xu, Teng Front Cell Infect Microbiol Cellular and Infection Microbiology Bacterial resistance to antibiotics has become an important concern for public health. This study was aimed to investigate the characteristics and the distribution of the florfenicol-related resistance genes in bacteria isolated from four farms. A total of 106 florfenicol-resistant Gram-negative bacilli were examined for florfenicol-related resistance genes, and the positive isolates were further characterized. The antimicrobial sensitivity results showed that most of them (100, 94.33%) belonged to multidrug resistance Enterobacteriaceae. About 91.51% of the strains carried floR gene, while 4.72% carried cfr gene. According to the pulsed-field gel electrophoresis results, 34 Escherichia coli were subdivided into 22 profiles, the genetic similarity coefficient of which ranged from 80.3 to 98.0%. The multilocus sequence typing (MLST) results revealed 17 sequence types (STs), with ST10 being the most prevalent. The genome sequencing result showed that the Proteus vulgaris G32 genome consists of a 4.06-Mb chromosome, a 177,911-bp plasmid (pG32-177), and a 51,686-bp plasmid (pG32-51). A floR located in a drug-resistant region on the chromosome of P. vulgaris G32 was with IS91 family transposase, and the other floR gene on the plasmid pG32-177 was with an ISCR2 insertion sequence. The cfr gene was located on the pG32-51 flanked by IS26 element and TnpA26. This study suggested that the mobile genetic elements played an important role in the replication of resistance genes and the horizontal resistance gene transfer. Frontiers Media S.A. 2020-07-31 /pmc/articles/PMC7438884/ /pubmed/32903722 http://dx.doi.org/10.3389/fcimb.2020.00369 Text en Copyright © 2020 Li, Zhu, Zhou, Lu, Liu, Sun, Ying, Lu, Lin, Li, Ying, Bao and Xu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Li, Peizhen
Zhu, Tingyuan
Zhou, Danying
Lu, Wei
Liu, Hongmao
Sun, Zhewei
Ying, Jun
Lu, Junwan
Lin, Xi
Li, Kewei
Ying, Jianchao
Bao, Qiyu
Xu, Teng
Analysis of Resistance to Florfenicol and the Related Mechanism of Dissemination in Different Animal-Derived Bacteria
title Analysis of Resistance to Florfenicol and the Related Mechanism of Dissemination in Different Animal-Derived Bacteria
title_full Analysis of Resistance to Florfenicol and the Related Mechanism of Dissemination in Different Animal-Derived Bacteria
title_fullStr Analysis of Resistance to Florfenicol and the Related Mechanism of Dissemination in Different Animal-Derived Bacteria
title_full_unstemmed Analysis of Resistance to Florfenicol and the Related Mechanism of Dissemination in Different Animal-Derived Bacteria
title_short Analysis of Resistance to Florfenicol and the Related Mechanism of Dissemination in Different Animal-Derived Bacteria
title_sort analysis of resistance to florfenicol and the related mechanism of dissemination in different animal-derived bacteria
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7438884/
https://www.ncbi.nlm.nih.gov/pubmed/32903722
http://dx.doi.org/10.3389/fcimb.2020.00369
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