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Molecular and functional architecture of the mouse photoreceptor network
Mouse photoreceptors are electrically coupled via gap junctions, but the relative importance of rod/rod, cone/cone, or rod/cone coupling is unknown. Furthermore, while connexin36 (Cx36) is expressed by cones, the identity of the rod connexin has been controversial. We report that FACS-sorted rods an...
Autores principales: | , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Association for the Advancement of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7439306/ https://www.ncbi.nlm.nih.gov/pubmed/32832605 http://dx.doi.org/10.1126/sciadv.aba7232 |
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author | Jin, Nange Zhang, Zhijing Keung, Joyce Youn, Sean B. Ishibashi, Munenori Tian, Lian-Ming Marshak, David W. Solessio, Eduardo Umino, Yumiko Fahrenfort, Iris Kiyama, Takae Mao, Chai-An You, Yanan Wei, Haichao Wu, Jiaqian Postma, Friso Paul, David L. Massey, Stephen C. Ribelayga, Christophe P. |
author_facet | Jin, Nange Zhang, Zhijing Keung, Joyce Youn, Sean B. Ishibashi, Munenori Tian, Lian-Ming Marshak, David W. Solessio, Eduardo Umino, Yumiko Fahrenfort, Iris Kiyama, Takae Mao, Chai-An You, Yanan Wei, Haichao Wu, Jiaqian Postma, Friso Paul, David L. Massey, Stephen C. Ribelayga, Christophe P. |
author_sort | Jin, Nange |
collection | PubMed |
description | Mouse photoreceptors are electrically coupled via gap junctions, but the relative importance of rod/rod, cone/cone, or rod/cone coupling is unknown. Furthermore, while connexin36 (Cx36) is expressed by cones, the identity of the rod connexin has been controversial. We report that FACS-sorted rods and cones both express Cx36 but no other connexins. We created rod- and cone-specific Cx36 knockout mice to dissect the photoreceptor network. In the wild type, Cx36 plaques at rod/cone contacts accounted for more than 95% of photoreceptor labeling and paired recordings showed the transjunctional conductance between rods and cones was ~300 pS. When Cx36 was eliminated on one side of the gap junction, in either conditional knockout, Cx36 labeling and rod/cone coupling were almost abolished. We could not detect direct rod/rod coupling, and cone/cone coupling was minor. Rod/cone coupling is so prevalent that indirect rod/cone/rod coupling via the network may account for previous reports of rod coupling. |
format | Online Article Text |
id | pubmed-7439306 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | American Association for the Advancement of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-74393062020-08-20 Molecular and functional architecture of the mouse photoreceptor network Jin, Nange Zhang, Zhijing Keung, Joyce Youn, Sean B. Ishibashi, Munenori Tian, Lian-Ming Marshak, David W. Solessio, Eduardo Umino, Yumiko Fahrenfort, Iris Kiyama, Takae Mao, Chai-An You, Yanan Wei, Haichao Wu, Jiaqian Postma, Friso Paul, David L. Massey, Stephen C. Ribelayga, Christophe P. Sci Adv Research Articles Mouse photoreceptors are electrically coupled via gap junctions, but the relative importance of rod/rod, cone/cone, or rod/cone coupling is unknown. Furthermore, while connexin36 (Cx36) is expressed by cones, the identity of the rod connexin has been controversial. We report that FACS-sorted rods and cones both express Cx36 but no other connexins. We created rod- and cone-specific Cx36 knockout mice to dissect the photoreceptor network. In the wild type, Cx36 plaques at rod/cone contacts accounted for more than 95% of photoreceptor labeling and paired recordings showed the transjunctional conductance between rods and cones was ~300 pS. When Cx36 was eliminated on one side of the gap junction, in either conditional knockout, Cx36 labeling and rod/cone coupling were almost abolished. We could not detect direct rod/rod coupling, and cone/cone coupling was minor. Rod/cone coupling is so prevalent that indirect rod/cone/rod coupling via the network may account for previous reports of rod coupling. American Association for the Advancement of Science 2020-07-08 /pmc/articles/PMC7439306/ /pubmed/32832605 http://dx.doi.org/10.1126/sciadv.aba7232 Text en Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). https://creativecommons.org/licenses/by-nc/4.0/ https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (https://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited. |
spellingShingle | Research Articles Jin, Nange Zhang, Zhijing Keung, Joyce Youn, Sean B. Ishibashi, Munenori Tian, Lian-Ming Marshak, David W. Solessio, Eduardo Umino, Yumiko Fahrenfort, Iris Kiyama, Takae Mao, Chai-An You, Yanan Wei, Haichao Wu, Jiaqian Postma, Friso Paul, David L. Massey, Stephen C. Ribelayga, Christophe P. Molecular and functional architecture of the mouse photoreceptor network |
title | Molecular and functional architecture of the mouse photoreceptor network |
title_full | Molecular and functional architecture of the mouse photoreceptor network |
title_fullStr | Molecular and functional architecture of the mouse photoreceptor network |
title_full_unstemmed | Molecular and functional architecture of the mouse photoreceptor network |
title_short | Molecular and functional architecture of the mouse photoreceptor network |
title_sort | molecular and functional architecture of the mouse photoreceptor network |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7439306/ https://www.ncbi.nlm.nih.gov/pubmed/32832605 http://dx.doi.org/10.1126/sciadv.aba7232 |
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