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Serum miR‐195‐5p is upregulated in gestational diabetes mellitus
BACKGROUND: Gestational diabetes mellitus (GDM) is defined as varying degrees of glucose intolerance with an onset or first recognition during pregnancy in women without previously diagnosed diabetes. Accumulating evidence indicates that miRNAs exert crucial roles in the pathogenesis and development...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7439337/ https://www.ncbi.nlm.nih.gov/pubmed/32301163 http://dx.doi.org/10.1002/jcla.23325 |
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author | Wang, Jianping Pan, Yuanyuan Dai, Fen Wang, Fan Qiu, Haifan Huang, Xianping |
author_facet | Wang, Jianping Pan, Yuanyuan Dai, Fen Wang, Fan Qiu, Haifan Huang, Xianping |
author_sort | Wang, Jianping |
collection | PubMed |
description | BACKGROUND: Gestational diabetes mellitus (GDM) is defined as varying degrees of glucose intolerance with an onset or first recognition during pregnancy in women without previously diagnosed diabetes. Accumulating evidence indicates that miRNAs exert crucial roles in the pathogenesis and development of diabetes, including GDM. In the present study, we aimed to determine the clinical performance of miR‐195‐5p in GDM. METHODS: First, the miR‐195‐5p expressions in serum samples from healthy pregnant women and women with GDM at 25 weeks pregnancy were detected using real‐time polymerase chain reaction (RT‐qPCR). Then, receive characteristic (ROC) curve was used to determine the diagnostic value of miR‐195‐5p in GDM. Finally, the correlation analysis of miR‐195‐5p expression with related clinicopathological factors was carried out to determine the clinical value of miR‐195‐5p in GDM. RESULTS: In this study, we found that miR‐195‐5p expression was significantly increased in serum samples from GDM patients as compared with that in healthy pregnancies. Furthermore, miR‐195‐5p might be a putative biomarker for GDM diagnosis with an area under the curve (AUC) of 0.8451; the cutoff value was 1.598, sensitivity was 73.69%, specificity was 96.85%, accuracy was 81.26%, and Youden index was 70.54%. Expression of miR‐195‐5p was positively associated with fasting plasma glucose, one‐hour plasma glucose, and two‐hour plasma glucose. CONCLUSION: miR‐195‐5p might function as a putative diagnostic biomarker for GDM and contribute to identifying at‐risk mothers in pregnancy. |
format | Online Article Text |
id | pubmed-7439337 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-74393372020-08-21 Serum miR‐195‐5p is upregulated in gestational diabetes mellitus Wang, Jianping Pan, Yuanyuan Dai, Fen Wang, Fan Qiu, Haifan Huang, Xianping J Clin Lab Anal Research Articles BACKGROUND: Gestational diabetes mellitus (GDM) is defined as varying degrees of glucose intolerance with an onset or first recognition during pregnancy in women without previously diagnosed diabetes. Accumulating evidence indicates that miRNAs exert crucial roles in the pathogenesis and development of diabetes, including GDM. In the present study, we aimed to determine the clinical performance of miR‐195‐5p in GDM. METHODS: First, the miR‐195‐5p expressions in serum samples from healthy pregnant women and women with GDM at 25 weeks pregnancy were detected using real‐time polymerase chain reaction (RT‐qPCR). Then, receive characteristic (ROC) curve was used to determine the diagnostic value of miR‐195‐5p in GDM. Finally, the correlation analysis of miR‐195‐5p expression with related clinicopathological factors was carried out to determine the clinical value of miR‐195‐5p in GDM. RESULTS: In this study, we found that miR‐195‐5p expression was significantly increased in serum samples from GDM patients as compared with that in healthy pregnancies. Furthermore, miR‐195‐5p might be a putative biomarker for GDM diagnosis with an area under the curve (AUC) of 0.8451; the cutoff value was 1.598, sensitivity was 73.69%, specificity was 96.85%, accuracy was 81.26%, and Youden index was 70.54%. Expression of miR‐195‐5p was positively associated with fasting plasma glucose, one‐hour plasma glucose, and two‐hour plasma glucose. CONCLUSION: miR‐195‐5p might function as a putative diagnostic biomarker for GDM and contribute to identifying at‐risk mothers in pregnancy. John Wiley and Sons Inc. 2020-04-17 /pmc/articles/PMC7439337/ /pubmed/32301163 http://dx.doi.org/10.1002/jcla.23325 Text en © 2020 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Wang, Jianping Pan, Yuanyuan Dai, Fen Wang, Fan Qiu, Haifan Huang, Xianping Serum miR‐195‐5p is upregulated in gestational diabetes mellitus |
title | Serum miR‐195‐5p is upregulated in gestational diabetes mellitus |
title_full | Serum miR‐195‐5p is upregulated in gestational diabetes mellitus |
title_fullStr | Serum miR‐195‐5p is upregulated in gestational diabetes mellitus |
title_full_unstemmed | Serum miR‐195‐5p is upregulated in gestational diabetes mellitus |
title_short | Serum miR‐195‐5p is upregulated in gestational diabetes mellitus |
title_sort | serum mir‐195‐5p is upregulated in gestational diabetes mellitus |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7439337/ https://www.ncbi.nlm.nih.gov/pubmed/32301163 http://dx.doi.org/10.1002/jcla.23325 |
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