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A long non‐coding RNA OLBC15 promotes triple‐negative breast cancer progression via enhancing ZNF326 degradation

BACKGROUND: The long non‐coding RNAs (lncRNAs) have been involved in various processes, including cancer. However, the function of many lncRNAs is still elusive in triple‐negative breast cancer (TNBC). METHODS: LncRNA profiling was used to screen for novel lncRNAs related to TNBC. OLBC15 expression...

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Detalles Bibliográficos
Autores principales: Deng, Chao, Zhang, Bojuan, Zhang, Yao, Xu, Xiaogang, Xiong, Deming, Chen, Xiaoyan, Wu, Jiaojiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7439339/
https://www.ncbi.nlm.nih.gov/pubmed/32329931
http://dx.doi.org/10.1002/jcla.23304
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author Deng, Chao
Zhang, Bojuan
Zhang, Yao
Xu, Xiaogang
Xiong, Deming
Chen, Xiaoyan
Wu, Jiaojiao
author_facet Deng, Chao
Zhang, Bojuan
Zhang, Yao
Xu, Xiaogang
Xiong, Deming
Chen, Xiaoyan
Wu, Jiaojiao
author_sort Deng, Chao
collection PubMed
description BACKGROUND: The long non‐coding RNAs (lncRNAs) have been involved in various processes, including cancer. However, the function of many lncRNAs is still elusive in triple‐negative breast cancer (TNBC). METHODS: LncRNA profiling was used to screen for novel lncRNAs related to TNBC. OLBC15 expression was measured via qRT‐PCR. In vitro migration and viability assays were conducted to determine the oncogenic role of OLBC15. Xenograft and metastatic models were performed to further investigate effects in vivo. RNA immunoprecipitation (RIP), mass spectrometry (MS), and fluorescence in situ hybridization (FISH) strategies were designed to identify the interaction between ZNF326 and OLBC15. RESULTS: In the current study, we have identified a novel oncogenic lncRNA termed OLBC15 via lncRNA profiling. OLBC15 is highly expressed especially in triple‐negative breast cancer. OLBC15 promoted viability and migration in breast cancer cells. Moreover, OLBC15 could accelerate metastasis and xenograft tumor growth. Mechanistic study suggested that OLBC15 could bind a well‐characterized tumor suppressor ZNF326 and OLBC15‐ZNF326 interaction resulted in ZNF326 destabilization. OLBC15 induced proteasomal ZNF326 degradation through enhanced ubiquitination. OLBC15 and ZNF326 protein expression is also negatively correlated in clinical specimens. CONCLUSIONS: Collectively, OLBC15 may serve as an oncogenic lncRNA to facilitate TNBC progression and a putative target for therapeutic anti‐breast cancer intervention.
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spelling pubmed-74393392020-08-21 A long non‐coding RNA OLBC15 promotes triple‐negative breast cancer progression via enhancing ZNF326 degradation Deng, Chao Zhang, Bojuan Zhang, Yao Xu, Xiaogang Xiong, Deming Chen, Xiaoyan Wu, Jiaojiao J Clin Lab Anal Research Articles BACKGROUND: The long non‐coding RNAs (lncRNAs) have been involved in various processes, including cancer. However, the function of many lncRNAs is still elusive in triple‐negative breast cancer (TNBC). METHODS: LncRNA profiling was used to screen for novel lncRNAs related to TNBC. OLBC15 expression was measured via qRT‐PCR. In vitro migration and viability assays were conducted to determine the oncogenic role of OLBC15. Xenograft and metastatic models were performed to further investigate effects in vivo. RNA immunoprecipitation (RIP), mass spectrometry (MS), and fluorescence in situ hybridization (FISH) strategies were designed to identify the interaction between ZNF326 and OLBC15. RESULTS: In the current study, we have identified a novel oncogenic lncRNA termed OLBC15 via lncRNA profiling. OLBC15 is highly expressed especially in triple‐negative breast cancer. OLBC15 promoted viability and migration in breast cancer cells. Moreover, OLBC15 could accelerate metastasis and xenograft tumor growth. Mechanistic study suggested that OLBC15 could bind a well‐characterized tumor suppressor ZNF326 and OLBC15‐ZNF326 interaction resulted in ZNF326 destabilization. OLBC15 induced proteasomal ZNF326 degradation through enhanced ubiquitination. OLBC15 and ZNF326 protein expression is also negatively correlated in clinical specimens. CONCLUSIONS: Collectively, OLBC15 may serve as an oncogenic lncRNA to facilitate TNBC progression and a putative target for therapeutic anti‐breast cancer intervention. John Wiley and Sons Inc. 2020-04-24 /pmc/articles/PMC7439339/ /pubmed/32329931 http://dx.doi.org/10.1002/jcla.23304 Text en © 2020 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals, Inc. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Research Articles
Deng, Chao
Zhang, Bojuan
Zhang, Yao
Xu, Xiaogang
Xiong, Deming
Chen, Xiaoyan
Wu, Jiaojiao
A long non‐coding RNA OLBC15 promotes triple‐negative breast cancer progression via enhancing ZNF326 degradation
title A long non‐coding RNA OLBC15 promotes triple‐negative breast cancer progression via enhancing ZNF326 degradation
title_full A long non‐coding RNA OLBC15 promotes triple‐negative breast cancer progression via enhancing ZNF326 degradation
title_fullStr A long non‐coding RNA OLBC15 promotes triple‐negative breast cancer progression via enhancing ZNF326 degradation
title_full_unstemmed A long non‐coding RNA OLBC15 promotes triple‐negative breast cancer progression via enhancing ZNF326 degradation
title_short A long non‐coding RNA OLBC15 promotes triple‐negative breast cancer progression via enhancing ZNF326 degradation
title_sort long non‐coding rna olbc15 promotes triple‐negative breast cancer progression via enhancing znf326 degradation
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7439339/
https://www.ncbi.nlm.nih.gov/pubmed/32329931
http://dx.doi.org/10.1002/jcla.23304
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