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Role of platelet infiltration as independent prognostic marker for gastric adenocarcinomas

BACKGROUND: In recent years, the relationship between malignant tumors and platelets has been paid more attention. The increase of platelets is an independent risk factor for the poor prognosis of some malignant tumors. METHODS: This study retrospectively analyzed the clinical and pathological data...

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Autores principales: Wang, Jing, Zhang, Miao, Zhou, Ting, Zhao, Shengmei, Su, Zhenguo, Liu, Xiangdong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7439343/
https://www.ncbi.nlm.nih.gov/pubmed/32233046
http://dx.doi.org/10.1002/jcla.23320
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author Wang, Jing
Zhang, Miao
Zhou, Ting
Zhao, Shengmei
Su, Zhenguo
Liu, Xiangdong
author_facet Wang, Jing
Zhang, Miao
Zhou, Ting
Zhao, Shengmei
Su, Zhenguo
Liu, Xiangdong
author_sort Wang, Jing
collection PubMed
description BACKGROUND: In recent years, the relationship between malignant tumors and platelets has been paid more attention. The increase of platelets is an independent risk factor for the poor prognosis of some malignant tumors. METHODS: This study retrospectively analyzed the clinical and pathological data of 114 patients with initial gastric cancer from August 2005 to August 2018 in Shandong Provincial Hospital. Single‐factor and multifactor survival analysis were used to evaluate the effect of platelet elevation on postoperative survival. The gastric cancer tissues of the Jinan Central Hospital and its matched paracancerous tissues were collected. The expression of platelets in tissues was detected by immunofluorescence technique. Different numbers of platelets were co‐cultured with MKN‐45 cells, CCK‐8 assay and transwell assay were performed, and the expression of epithelial‐mesenchymal transition‐related proteins was detected. RESULTS: Platelet count was independent factors affecting prognosis. The stratified analysis showed that there was a statistically significant difference in the 5‐year survival rate between the platelet‐increase group and the normal platelet group in the TNM stages I‐II. The expression of platelets in gastric cancer tissues was higher than that in adjacent tissues. The results of CCK‐8 and transwell showed that platelets significantly enhanced the proliferation and metastasis capability of MKN‐45 cells in a concentration‐dependent manner. After co‐culture, the expression level of E‐cadherin protein in MKN‐45 cells decreased, and the protein expression levels of N‐cadherin, vimentin, and VEGFA increased. CONCLUSION: Platelet elevation is closely related to the occurrence, development, and metastasis of gastric cancer, and platelet count can be used as a prognostic indicator for malignant tumors.
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spelling pubmed-74393432020-08-21 Role of platelet infiltration as independent prognostic marker for gastric adenocarcinomas Wang, Jing Zhang, Miao Zhou, Ting Zhao, Shengmei Su, Zhenguo Liu, Xiangdong J Clin Lab Anal Research Articles BACKGROUND: In recent years, the relationship between malignant tumors and platelets has been paid more attention. The increase of platelets is an independent risk factor for the poor prognosis of some malignant tumors. METHODS: This study retrospectively analyzed the clinical and pathological data of 114 patients with initial gastric cancer from August 2005 to August 2018 in Shandong Provincial Hospital. Single‐factor and multifactor survival analysis were used to evaluate the effect of platelet elevation on postoperative survival. The gastric cancer tissues of the Jinan Central Hospital and its matched paracancerous tissues were collected. The expression of platelets in tissues was detected by immunofluorescence technique. Different numbers of platelets were co‐cultured with MKN‐45 cells, CCK‐8 assay and transwell assay were performed, and the expression of epithelial‐mesenchymal transition‐related proteins was detected. RESULTS: Platelet count was independent factors affecting prognosis. The stratified analysis showed that there was a statistically significant difference in the 5‐year survival rate between the platelet‐increase group and the normal platelet group in the TNM stages I‐II. The expression of platelets in gastric cancer tissues was higher than that in adjacent tissues. The results of CCK‐8 and transwell showed that platelets significantly enhanced the proliferation and metastasis capability of MKN‐45 cells in a concentration‐dependent manner. After co‐culture, the expression level of E‐cadherin protein in MKN‐45 cells decreased, and the protein expression levels of N‐cadherin, vimentin, and VEGFA increased. CONCLUSION: Platelet elevation is closely related to the occurrence, development, and metastasis of gastric cancer, and platelet count can be used as a prognostic indicator for malignant tumors. John Wiley and Sons Inc. 2020-03-31 /pmc/articles/PMC7439343/ /pubmed/32233046 http://dx.doi.org/10.1002/jcla.23320 Text en © 2020 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals, Inc. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Research Articles
Wang, Jing
Zhang, Miao
Zhou, Ting
Zhao, Shengmei
Su, Zhenguo
Liu, Xiangdong
Role of platelet infiltration as independent prognostic marker for gastric adenocarcinomas
title Role of platelet infiltration as independent prognostic marker for gastric adenocarcinomas
title_full Role of platelet infiltration as independent prognostic marker for gastric adenocarcinomas
title_fullStr Role of platelet infiltration as independent prognostic marker for gastric adenocarcinomas
title_full_unstemmed Role of platelet infiltration as independent prognostic marker for gastric adenocarcinomas
title_short Role of platelet infiltration as independent prognostic marker for gastric adenocarcinomas
title_sort role of platelet infiltration as independent prognostic marker for gastric adenocarcinomas
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7439343/
https://www.ncbi.nlm.nih.gov/pubmed/32233046
http://dx.doi.org/10.1002/jcla.23320
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