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Visualizing Nudivirus Assembly and Egress

Enveloped viruses hijack cellular membranes in order to provide the necessary material for virion assembly. In particular, viruses that replicate and assemble inside the nucleus have developed special approaches to modify the nuclear landscape for their advantage. We used electron microscopy to inve...

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Detalles Bibliográficos
Autores principales: Velamoor, Sailakshmi, Mitchell, Allan, Humbel, Bruno M., Kim, WonMo, Pushparajan, Charlotte, Visnovsky, Gabriel, Burga, Laura N., Bostina, Mihnea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7439470/
https://www.ncbi.nlm.nih.gov/pubmed/32788378
http://dx.doi.org/10.1128/mBio.01333-20
Descripción
Sumario:Enveloped viruses hijack cellular membranes in order to provide the necessary material for virion assembly. In particular, viruses that replicate and assemble inside the nucleus have developed special approaches to modify the nuclear landscape for their advantage. We used electron microscopy to investigate cellular changes occurring during nudivirus infection and we characterized a unique mechanism for assembly, packaging, and transport of new virions across the nuclear membrane and through the cytoplasm. Our three-dimensional reconstructions describe the complex remodeling of the nuclear membrane necessary to release vesicle-associated viruses into the cytoplasm. This is the first report of nuclear morphological reconfigurations that occur during nudiviral infection.