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Chimeric apoptotic bodies functionalized with natural membrane and modular delivery system for inflammation modulation

Engineered extracellular vesicles (EVs) carrying therapeutic molecules are promising candidates for disease therapies. Yet, engineering EVs with optimal functions is a challenge that requires careful selection of functionally specific vesicles and a proper engineering strategy. Here, we constructed...

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Autores principales: Dou, Geng, Tian, Ran, Liu, Xuemei, Yuan, Pingyun, Ye, Qianwen, Liu, Jin, Liu, Siying, Zhou, Jun, Deng, Zhihong, Chen, Xin, Liu, Shiyu, Jin, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7439513/
https://www.ncbi.nlm.nih.gov/pubmed/32832662
http://dx.doi.org/10.1126/sciadv.aba2987
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author Dou, Geng
Tian, Ran
Liu, Xuemei
Yuan, Pingyun
Ye, Qianwen
Liu, Jin
Liu, Siying
Zhou, Jun
Deng, Zhihong
Chen, Xin
Liu, Shiyu
Jin, Yan
author_facet Dou, Geng
Tian, Ran
Liu, Xuemei
Yuan, Pingyun
Ye, Qianwen
Liu, Jin
Liu, Siying
Zhou, Jun
Deng, Zhihong
Chen, Xin
Liu, Shiyu
Jin, Yan
author_sort Dou, Geng
collection PubMed
description Engineered extracellular vesicles (EVs) carrying therapeutic molecules are promising candidates for disease therapies. Yet, engineering EVs with optimal functions is a challenge that requires careful selection of functionally specific vesicles and a proper engineering strategy. Here, we constructed chimeric apoptotic bodies (cABs) for on-demand inflammation modulation by combining pure membrane from apoptotic bodies (ABs) as a bioconjugation/regulation module and mesoporous silica nanoparticles (MSNs) as a carrier module. MSNs were preloaded with anti-inflammatory agents (microRNA-21 or curcumin) and modified with stimuli-responsive molecules to achieve accurate cargo release at designated locations. The resulting cABs actively target macrophages in the inflammatory region and effectively promote M2 polarization of these macrophages to modulate inflammation due to the synergistic regulatory effects of AB membranes and the intracellular release of preloaded cargos. This work provides strategies to arbitrarily engineer modular EVs that integrate the advantages of natural EVs and synthetic materials for various applications.
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spelling pubmed-74395132020-08-20 Chimeric apoptotic bodies functionalized with natural membrane and modular delivery system for inflammation modulation Dou, Geng Tian, Ran Liu, Xuemei Yuan, Pingyun Ye, Qianwen Liu, Jin Liu, Siying Zhou, Jun Deng, Zhihong Chen, Xin Liu, Shiyu Jin, Yan Sci Adv Research Articles Engineered extracellular vesicles (EVs) carrying therapeutic molecules are promising candidates for disease therapies. Yet, engineering EVs with optimal functions is a challenge that requires careful selection of functionally specific vesicles and a proper engineering strategy. Here, we constructed chimeric apoptotic bodies (cABs) for on-demand inflammation modulation by combining pure membrane from apoptotic bodies (ABs) as a bioconjugation/regulation module and mesoporous silica nanoparticles (MSNs) as a carrier module. MSNs were preloaded with anti-inflammatory agents (microRNA-21 or curcumin) and modified with stimuli-responsive molecules to achieve accurate cargo release at designated locations. The resulting cABs actively target macrophages in the inflammatory region and effectively promote M2 polarization of these macrophages to modulate inflammation due to the synergistic regulatory effects of AB membranes and the intracellular release of preloaded cargos. This work provides strategies to arbitrarily engineer modular EVs that integrate the advantages of natural EVs and synthetic materials for various applications. American Association for the Advancement of Science 2020-07-22 /pmc/articles/PMC7439513/ /pubmed/32832662 http://dx.doi.org/10.1126/sciadv.aba2987 Text en Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). https://creativecommons.org/licenses/by-nc/4.0/ https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (https://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
spellingShingle Research Articles
Dou, Geng
Tian, Ran
Liu, Xuemei
Yuan, Pingyun
Ye, Qianwen
Liu, Jin
Liu, Siying
Zhou, Jun
Deng, Zhihong
Chen, Xin
Liu, Shiyu
Jin, Yan
Chimeric apoptotic bodies functionalized with natural membrane and modular delivery system for inflammation modulation
title Chimeric apoptotic bodies functionalized with natural membrane and modular delivery system for inflammation modulation
title_full Chimeric apoptotic bodies functionalized with natural membrane and modular delivery system for inflammation modulation
title_fullStr Chimeric apoptotic bodies functionalized with natural membrane and modular delivery system for inflammation modulation
title_full_unstemmed Chimeric apoptotic bodies functionalized with natural membrane and modular delivery system for inflammation modulation
title_short Chimeric apoptotic bodies functionalized with natural membrane and modular delivery system for inflammation modulation
title_sort chimeric apoptotic bodies functionalized with natural membrane and modular delivery system for inflammation modulation
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7439513/
https://www.ncbi.nlm.nih.gov/pubmed/32832662
http://dx.doi.org/10.1126/sciadv.aba2987
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