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Oleic Acid, Cholesterol, and Linoleic Acid as Angiogenesis Initiators
The current study determined the natural angiogenic molecules using an unbiased metabolomics approach. A chick chorioallantoic membrane (CAM) model was used to examine pro- and antiangiogenic molecules, followed by gas chromatography–mass spectrometry (GCMS) analysis. Vessel formation was analyzed q...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7439708/ https://www.ncbi.nlm.nih.gov/pubmed/32832811 http://dx.doi.org/10.1021/acsomega.0c02850 |
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author | Samson, Faith Pwaniyibo Patrick, Ambrose Teru Fabunmi, Tosin Esther Yahaya, Muhammad Falalu Madu, Joshua He, Weilue Sripathi, Srinivas R. Tyndall, Jennifer Raji, Hayatu Jee, Donghyun Gutsaeva, Diana R. Jahng, Wan Jin |
author_facet | Samson, Faith Pwaniyibo Patrick, Ambrose Teru Fabunmi, Tosin Esther Yahaya, Muhammad Falalu Madu, Joshua He, Weilue Sripathi, Srinivas R. Tyndall, Jennifer Raji, Hayatu Jee, Donghyun Gutsaeva, Diana R. Jahng, Wan Jin |
author_sort | Samson, Faith Pwaniyibo |
collection | PubMed |
description | The current study determined the natural angiogenic molecules using an unbiased metabolomics approach. A chick chorioallantoic membrane (CAM) model was used to examine pro- and antiangiogenic molecules, followed by gas chromatography–mass spectrometry (GCMS) analysis. Vessel formation was analyzed quantitatively using the angiogenic index (p < 0.05). At embryonic day one, a white streak or circle area was observed when vessel formation begins. GCMS analysis and database search demonstrated that angiogenesis may initiate when oleic, cholesterol, and linoleic acids increased in the area of angiogenic reactions. The gain of function study was conducted by the injection of cholesterol and oleic acid into a chick embryo to determine the role of each lipid in angiogenesis. We propose that oleic acid, cholesterol, and linoleic acid are natural molecules that set the platform for the initiation stage of angiogenesis before other proteins including the vascular endothelial growth factor, angiopoietin, angiotensin, and erythropoietin join as the angiome in sprout extension and vessel maturation. |
format | Online Article Text |
id | pubmed-7439708 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-74397082020-08-21 Oleic Acid, Cholesterol, and Linoleic Acid as Angiogenesis Initiators Samson, Faith Pwaniyibo Patrick, Ambrose Teru Fabunmi, Tosin Esther Yahaya, Muhammad Falalu Madu, Joshua He, Weilue Sripathi, Srinivas R. Tyndall, Jennifer Raji, Hayatu Jee, Donghyun Gutsaeva, Diana R. Jahng, Wan Jin ACS Omega The current study determined the natural angiogenic molecules using an unbiased metabolomics approach. A chick chorioallantoic membrane (CAM) model was used to examine pro- and antiangiogenic molecules, followed by gas chromatography–mass spectrometry (GCMS) analysis. Vessel formation was analyzed quantitatively using the angiogenic index (p < 0.05). At embryonic day one, a white streak or circle area was observed when vessel formation begins. GCMS analysis and database search demonstrated that angiogenesis may initiate when oleic, cholesterol, and linoleic acids increased in the area of angiogenic reactions. The gain of function study was conducted by the injection of cholesterol and oleic acid into a chick embryo to determine the role of each lipid in angiogenesis. We propose that oleic acid, cholesterol, and linoleic acid are natural molecules that set the platform for the initiation stage of angiogenesis before other proteins including the vascular endothelial growth factor, angiopoietin, angiotensin, and erythropoietin join as the angiome in sprout extension and vessel maturation. American Chemical Society 2020-08-10 /pmc/articles/PMC7439708/ /pubmed/32832811 http://dx.doi.org/10.1021/acsomega.0c02850 Text en Copyright © 2020 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes. |
spellingShingle | Samson, Faith Pwaniyibo Patrick, Ambrose Teru Fabunmi, Tosin Esther Yahaya, Muhammad Falalu Madu, Joshua He, Weilue Sripathi, Srinivas R. Tyndall, Jennifer Raji, Hayatu Jee, Donghyun Gutsaeva, Diana R. Jahng, Wan Jin Oleic Acid, Cholesterol, and Linoleic Acid as Angiogenesis Initiators |
title | Oleic Acid, Cholesterol, and
Linoleic Acid as Angiogenesis
Initiators |
title_full | Oleic Acid, Cholesterol, and
Linoleic Acid as Angiogenesis
Initiators |
title_fullStr | Oleic Acid, Cholesterol, and
Linoleic Acid as Angiogenesis
Initiators |
title_full_unstemmed | Oleic Acid, Cholesterol, and
Linoleic Acid as Angiogenesis
Initiators |
title_short | Oleic Acid, Cholesterol, and
Linoleic Acid as Angiogenesis
Initiators |
title_sort | oleic acid, cholesterol, and
linoleic acid as angiogenesis
initiators |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7439708/ https://www.ncbi.nlm.nih.gov/pubmed/32832811 http://dx.doi.org/10.1021/acsomega.0c02850 |
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