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Constructing gene network for type 1 narcolepsy based on genome-wide association study and differential gene expression analysis (STROBE)

Although many genes that affect narcolepsy risk have been identified, the interactions among these genes are still unclear. Moreover, there is a lack of research on the construction of the genetic network of narcolepsy. To screen candidate genes related to the onset of narcolepsy type 1, the functio...

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Autores principales: Ouyang, Hui, Wang, Shiying, Zheng, Qiwen, Zhang, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer Health 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7440059/
https://www.ncbi.nlm.nih.gov/pubmed/32358372
http://dx.doi.org/10.1097/MD.0000000000019985
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author Ouyang, Hui
Wang, Shiying
Zheng, Qiwen
Zhang, Jun
author_facet Ouyang, Hui
Wang, Shiying
Zheng, Qiwen
Zhang, Jun
author_sort Ouyang, Hui
collection PubMed
description Although many genes that affect narcolepsy risk have been identified, the interactions among these genes are still unclear. Moreover, there is a lack of research on the construction of the genetic network of narcolepsy. To screen candidate genes related to the onset of narcolepsy type 1, the function and distribution of important genes related to narcolepsy type 1 were studied and a gene network was constructed to study the pathogenesis of narcolepsy type 1. A case-control study (observational study) of 1075 Chinese narcoleptic patients and 1997 controls was conducted. The gene-sequencing data was analyzed using genome-wide association analysis. The candidate genes related to narcolepsy were identified by differential gene expression analysis and literature research. Then, the 28 candidate genes were input into the KEGG database and 32 pathway data related to candidate genes were obtained. A gene network, with the pathways as links and the genes as nodes, was constructed. According to our results, TNF, MHC II, NFATC2, and CXCL8 were the top genes in the gene network. TNF, MHC II, NFATC2, and CXCL8 are closely related to narcolepsy type I and require further study. By analyzing the pathways of disease-related genes and the network of gene interaction, we can provide an outlinefor the study of specific mechanisms of and treatments for narcolepsy.
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spelling pubmed-74400592020-09-04 Constructing gene network for type 1 narcolepsy based on genome-wide association study and differential gene expression analysis (STROBE) Ouyang, Hui Wang, Shiying Zheng, Qiwen Zhang, Jun Medicine (Baltimore) 3500 Although many genes that affect narcolepsy risk have been identified, the interactions among these genes are still unclear. Moreover, there is a lack of research on the construction of the genetic network of narcolepsy. To screen candidate genes related to the onset of narcolepsy type 1, the function and distribution of important genes related to narcolepsy type 1 were studied and a gene network was constructed to study the pathogenesis of narcolepsy type 1. A case-control study (observational study) of 1075 Chinese narcoleptic patients and 1997 controls was conducted. The gene-sequencing data was analyzed using genome-wide association analysis. The candidate genes related to narcolepsy were identified by differential gene expression analysis and literature research. Then, the 28 candidate genes were input into the KEGG database and 32 pathway data related to candidate genes were obtained. A gene network, with the pathways as links and the genes as nodes, was constructed. According to our results, TNF, MHC II, NFATC2, and CXCL8 were the top genes in the gene network. TNF, MHC II, NFATC2, and CXCL8 are closely related to narcolepsy type I and require further study. By analyzing the pathways of disease-related genes and the network of gene interaction, we can provide an outlinefor the study of specific mechanisms of and treatments for narcolepsy. Wolters Kluwer Health 2020-05-01 /pmc/articles/PMC7440059/ /pubmed/32358372 http://dx.doi.org/10.1097/MD.0000000000019985 Text en Copyright © 2020 the Author(s). Published by Wolters Kluwer Health, Inc. http://creativecommons.org/licenses/by/4.0 This is an open access article distributed under the Creative Commons Attribution License 4.0 (CCBY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. http://creativecommons.org/licenses/by/4.0
spellingShingle 3500
Ouyang, Hui
Wang, Shiying
Zheng, Qiwen
Zhang, Jun
Constructing gene network for type 1 narcolepsy based on genome-wide association study and differential gene expression analysis (STROBE)
title Constructing gene network for type 1 narcolepsy based on genome-wide association study and differential gene expression analysis (STROBE)
title_full Constructing gene network for type 1 narcolepsy based on genome-wide association study and differential gene expression analysis (STROBE)
title_fullStr Constructing gene network for type 1 narcolepsy based on genome-wide association study and differential gene expression analysis (STROBE)
title_full_unstemmed Constructing gene network for type 1 narcolepsy based on genome-wide association study and differential gene expression analysis (STROBE)
title_short Constructing gene network for type 1 narcolepsy based on genome-wide association study and differential gene expression analysis (STROBE)
title_sort constructing gene network for type 1 narcolepsy based on genome-wide association study and differential gene expression analysis (strobe)
topic 3500
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7440059/
https://www.ncbi.nlm.nih.gov/pubmed/32358372
http://dx.doi.org/10.1097/MD.0000000000019985
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