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A HSV1 mutant leads to an attenuated phenotype and induces immunity with a protective effect

Herpes simplex virus type 1 (HSV1) is a complicated structural agent with a sophisticated transcription process and a high infection rate. A vaccine against HSV1 is urgently needed. As multiple viral-encoded proteins, including structural and nonstructural proteins, contribute to immune response sti...

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Autores principales: Xu, Xingli, Feng, Xiao, Wang, Lichun, Yi, Ting, Zheng, Lichun, Jiang, Guorun, Fan, Shengtao, Liao, Yun, Feng, Min, Zhang, Ying, Li, Dandan, Li, Qihan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7440667/
https://www.ncbi.nlm.nih.gov/pubmed/32776994
http://dx.doi.org/10.1371/journal.ppat.1008703
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author Xu, Xingli
Feng, Xiao
Wang, Lichun
Yi, Ting
Zheng, Lichun
Jiang, Guorun
Fan, Shengtao
Liao, Yun
Feng, Min
Zhang, Ying
Li, Dandan
Li, Qihan
author_facet Xu, Xingli
Feng, Xiao
Wang, Lichun
Yi, Ting
Zheng, Lichun
Jiang, Guorun
Fan, Shengtao
Liao, Yun
Feng, Min
Zhang, Ying
Li, Dandan
Li, Qihan
author_sort Xu, Xingli
collection PubMed
description Herpes simplex virus type 1 (HSV1) is a complicated structural agent with a sophisticated transcription process and a high infection rate. A vaccine against HSV1 is urgently needed. As multiple viral-encoded proteins, including structural and nonstructural proteins, contribute to immune response stimulation, an attenuated or deficient HSV1 vaccine may be relatively reliable. Advances in genomic modification technologies provide reliable means of constructing various HSV vaccine candidates. Based on our previous work, an M6 mutant with mutations in the UL7, UL41, LAT, Us3, Us11 and Us12 genes was established. The mutant exhibited low proliferation in cells and an attenuated phenotype in an animal model. Furthermore, in mice and rhesus monkeys, the mutant can induce remarkable serum neutralizing antibody titers and T cell activation and protect against HSV1 challenge by impeding viral replication, dissemination and pathogenesis.
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spelling pubmed-74406672020-08-26 A HSV1 mutant leads to an attenuated phenotype and induces immunity with a protective effect Xu, Xingli Feng, Xiao Wang, Lichun Yi, Ting Zheng, Lichun Jiang, Guorun Fan, Shengtao Liao, Yun Feng, Min Zhang, Ying Li, Dandan Li, Qihan PLoS Pathog Research Article Herpes simplex virus type 1 (HSV1) is a complicated structural agent with a sophisticated transcription process and a high infection rate. A vaccine against HSV1 is urgently needed. As multiple viral-encoded proteins, including structural and nonstructural proteins, contribute to immune response stimulation, an attenuated or deficient HSV1 vaccine may be relatively reliable. Advances in genomic modification technologies provide reliable means of constructing various HSV vaccine candidates. Based on our previous work, an M6 mutant with mutations in the UL7, UL41, LAT, Us3, Us11 and Us12 genes was established. The mutant exhibited low proliferation in cells and an attenuated phenotype in an animal model. Furthermore, in mice and rhesus monkeys, the mutant can induce remarkable serum neutralizing antibody titers and T cell activation and protect against HSV1 challenge by impeding viral replication, dissemination and pathogenesis. Public Library of Science 2020-08-10 /pmc/articles/PMC7440667/ /pubmed/32776994 http://dx.doi.org/10.1371/journal.ppat.1008703 Text en © 2020 Xu et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Xu, Xingli
Feng, Xiao
Wang, Lichun
Yi, Ting
Zheng, Lichun
Jiang, Guorun
Fan, Shengtao
Liao, Yun
Feng, Min
Zhang, Ying
Li, Dandan
Li, Qihan
A HSV1 mutant leads to an attenuated phenotype and induces immunity with a protective effect
title A HSV1 mutant leads to an attenuated phenotype and induces immunity with a protective effect
title_full A HSV1 mutant leads to an attenuated phenotype and induces immunity with a protective effect
title_fullStr A HSV1 mutant leads to an attenuated phenotype and induces immunity with a protective effect
title_full_unstemmed A HSV1 mutant leads to an attenuated phenotype and induces immunity with a protective effect
title_short A HSV1 mutant leads to an attenuated phenotype and induces immunity with a protective effect
title_sort hsv1 mutant leads to an attenuated phenotype and induces immunity with a protective effect
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7440667/
https://www.ncbi.nlm.nih.gov/pubmed/32776994
http://dx.doi.org/10.1371/journal.ppat.1008703
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