CD89/CD35 Expression Ratio in Salivary Neutrophil as an Early Detection Marker for Severe Early Childhood Caries

Objectives  To analyze CD35/CD89 expression ratio on the surface of neutrophils as an early detection marker for S-ECC. Materials and Methods  Saliva was collected from 4- to 6-year-old kindergarten students. Salivary neutrophils were obtained by instructing the subjects to rinse their mouth with 1...

Descripción completa

Detalles Bibliográficos
Autores principales: Luthfi, Muhammad, Oki, Aqsa Sjuhada, Indrawati, Retno, Rifai, Muhaimin, Dachlan, Yoes Prijatna, Razak, Fathilah Abdul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Thieme Medical and Scientific Publishers Private Ltd. 2020
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7440938/
https://www.ncbi.nlm.nih.gov/pubmed/32645730
http://dx.doi.org/10.1055/s-0040-1713704
_version_ 1783573211371274240
author Luthfi, Muhammad
Oki, Aqsa Sjuhada
Indrawati, Retno
Rifai, Muhaimin
Dachlan, Yoes Prijatna
Razak, Fathilah Abdul
author_facet Luthfi, Muhammad
Oki, Aqsa Sjuhada
Indrawati, Retno
Rifai, Muhaimin
Dachlan, Yoes Prijatna
Razak, Fathilah Abdul
author_sort Luthfi, Muhammad
collection PubMed
description Objectives  To analyze CD35/CD89 expression ratio on the surface of neutrophils as an early detection marker for S-ECC. Materials and Methods  Saliva was collected from 4- to 6-year-old kindergarten students. Salivary neutrophils were obtained by instructing the subjects to rinse their mouth with 1 mL of sterile 1.5% NaCl for 30 seconds before expectorating it into a sterile glass. The expression of CFSE (+) CD35 (+) and CFSE (+) CD89 (+) was measured and analyzed using flow cytometry. Results The expression of CFSE (+) CD89 (+) in the caries-free group (2.46 ± 0.39) was significantly lower than that in the S-ECC group (3.41 ± 1.11), with a p -value of 0.0001, while the expression of CFSE (+) CD35 (+) in the caries-free group was (2.35 ± 0.56) compared with (1.54 ± 0.35) ( p = 0.0001) in the S-ECC group. Conclusions  The expression ratio of CFSE (+) CD89 (+) and CFSE (+) CD35 (+) constitutes a marker for S-ECC.
format Online
Article
Text
id pubmed-7440938
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Thieme Medical and Scientific Publishers Private Ltd.
record_format MEDLINE/PubMed
spelling pubmed-74409382020-08-25 CD89/CD35 Expression Ratio in Salivary Neutrophil as an Early Detection Marker for Severe Early Childhood Caries Luthfi, Muhammad Oki, Aqsa Sjuhada Indrawati, Retno Rifai, Muhaimin Dachlan, Yoes Prijatna Razak, Fathilah Abdul Eur J Dent Objectives  To analyze CD35/CD89 expression ratio on the surface of neutrophils as an early detection marker for S-ECC. Materials and Methods  Saliva was collected from 4- to 6-year-old kindergarten students. Salivary neutrophils were obtained by instructing the subjects to rinse their mouth with 1 mL of sterile 1.5% NaCl for 30 seconds before expectorating it into a sterile glass. The expression of CFSE (+) CD35 (+) and CFSE (+) CD89 (+) was measured and analyzed using flow cytometry. Results The expression of CFSE (+) CD89 (+) in the caries-free group (2.46 ± 0.39) was significantly lower than that in the S-ECC group (3.41 ± 1.11), with a p -value of 0.0001, while the expression of CFSE (+) CD35 (+) in the caries-free group was (2.35 ± 0.56) compared with (1.54 ± 0.35) ( p = 0.0001) in the S-ECC group. Conclusions  The expression ratio of CFSE (+) CD89 (+) and CFSE (+) CD35 (+) constitutes a marker for S-ECC. Thieme Medical and Scientific Publishers Private Ltd. 2020-07 2020-07-09 /pmc/articles/PMC7440938/ /pubmed/32645730 http://dx.doi.org/10.1055/s-0040-1713704 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License, which permits unrestricted reproduction and distribution, for non-commercial purposes only; and use and reproduction, but not distribution, of adapted material for non-commercial purposes only, provided the original work is properly cited.
spellingShingle Luthfi, Muhammad
Oki, Aqsa Sjuhada
Indrawati, Retno
Rifai, Muhaimin
Dachlan, Yoes Prijatna
Razak, Fathilah Abdul
CD89/CD35 Expression Ratio in Salivary Neutrophil as an Early Detection Marker for Severe Early Childhood Caries
title CD89/CD35 Expression Ratio in Salivary Neutrophil as an Early Detection Marker for Severe Early Childhood Caries
title_full CD89/CD35 Expression Ratio in Salivary Neutrophil as an Early Detection Marker for Severe Early Childhood Caries
title_fullStr CD89/CD35 Expression Ratio in Salivary Neutrophil as an Early Detection Marker for Severe Early Childhood Caries
title_full_unstemmed CD89/CD35 Expression Ratio in Salivary Neutrophil as an Early Detection Marker for Severe Early Childhood Caries
title_short CD89/CD35 Expression Ratio in Salivary Neutrophil as an Early Detection Marker for Severe Early Childhood Caries
title_sort cd89/cd35 expression ratio in salivary neutrophil as an early detection marker for severe early childhood caries
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7440938/
https://www.ncbi.nlm.nih.gov/pubmed/32645730
http://dx.doi.org/10.1055/s-0040-1713704
work_keys_str_mv AT luthfimuhammad cd89cd35expressionratioinsalivaryneutrophilasanearlydetectionmarkerforsevereearlychildhoodcaries
AT okiaqsasjuhada cd89cd35expressionratioinsalivaryneutrophilasanearlydetectionmarkerforsevereearlychildhoodcaries
AT indrawatiretno cd89cd35expressionratioinsalivaryneutrophilasanearlydetectionmarkerforsevereearlychildhoodcaries
AT rifaimuhaimin cd89cd35expressionratioinsalivaryneutrophilasanearlydetectionmarkerforsevereearlychildhoodcaries
AT dachlanyoesprijatna cd89cd35expressionratioinsalivaryneutrophilasanearlydetectionmarkerforsevereearlychildhoodcaries
AT razakfathilahabdul cd89cd35expressionratioinsalivaryneutrophilasanearlydetectionmarkerforsevereearlychildhoodcaries

Ejemplares similares