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Quantification and Pathogenicity of Candida albicans in Denture-Wearing and Nondenture-Wearing Elderly
Objective The primary purpose of this study was to evaluate and compare the microbial loads and pathogenicity traits of oral Candida albicans in denture-wearing (DW; n = 15) and nondenture-wearing (NDW; n = 15) elderly persons. Materials and Methods The fungal counts of the saliva, tongue dorsa, a...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Thieme Medical and Scientific Publishers Private Ltd.
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7440952/ https://www.ncbi.nlm.nih.gov/pubmed/32542630 http://dx.doi.org/10.1055/s-0040-1712779 |
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author | Bachtiar, Boy M. Fath, Turmidzi Widowati, Retno Bachtiar, Endang W. |
author_facet | Bachtiar, Boy M. Fath, Turmidzi Widowati, Retno Bachtiar, Endang W. |
author_sort | Bachtiar, Boy M. |
collection | PubMed |
description | Objective The primary purpose of this study was to evaluate and compare the microbial loads and pathogenicity traits of oral Candida albicans in denture-wearing (DW; n = 15) and nondenture-wearing (NDW; n = 15) elderly persons. Materials and Methods The fungal counts of the saliva, tongue dorsa, and prosthesis-fitting surfaces of the participants were assessed using real-time polymerase chain reaction to compare the quantity and expression of selected C. albicans biofilm-associated genes ( ALS3 , HWP1 , and YWP1 ). Statistical Analysis The obtained data were analyzed by one-way analysis of variance, followed by Bartlett’s test. When appropriate, the Student’s t -test was also used; a value of p < 0.05 was considered statistically significant. Results In both groups, the count of C. albicans was found to be significantly higher in saliva than in other oral samples. The expression of the hypha-specific genes ( ALS3 and HWP1 ) in the tongue dorsa was higher in the DW group ( p < 0.05), whereas the transcription level of the yeast-specific gene ( YWP1) was significantly higher in the NDW group. Conclusion Both tongue dorsa and dentures appear to be sharing factors that are important for C. albicans biofilm growth in abiotic and biotic oral surfaces of the elderly. |
format | Online Article Text |
id | pubmed-7440952 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Thieme Medical and Scientific Publishers Private Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-74409522020-08-25 Quantification and Pathogenicity of Candida albicans in Denture-Wearing and Nondenture-Wearing Elderly Bachtiar, Boy M. Fath, Turmidzi Widowati, Retno Bachtiar, Endang W. Eur J Dent Objective The primary purpose of this study was to evaluate and compare the microbial loads and pathogenicity traits of oral Candida albicans in denture-wearing (DW; n = 15) and nondenture-wearing (NDW; n = 15) elderly persons. Materials and Methods The fungal counts of the saliva, tongue dorsa, and prosthesis-fitting surfaces of the participants were assessed using real-time polymerase chain reaction to compare the quantity and expression of selected C. albicans biofilm-associated genes ( ALS3 , HWP1 , and YWP1 ). Statistical Analysis The obtained data were analyzed by one-way analysis of variance, followed by Bartlett’s test. When appropriate, the Student’s t -test was also used; a value of p < 0.05 was considered statistically significant. Results In both groups, the count of C. albicans was found to be significantly higher in saliva than in other oral samples. The expression of the hypha-specific genes ( ALS3 and HWP1 ) in the tongue dorsa was higher in the DW group ( p < 0.05), whereas the transcription level of the yeast-specific gene ( YWP1) was significantly higher in the NDW group. Conclusion Both tongue dorsa and dentures appear to be sharing factors that are important for C. albicans biofilm growth in abiotic and biotic oral surfaces of the elderly. Thieme Medical and Scientific Publishers Private Ltd. 2020-07 2020-06-15 /pmc/articles/PMC7440952/ /pubmed/32542630 http://dx.doi.org/10.1055/s-0040-1712779 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License, which permits unrestricted reproduction and distribution, for non-commercial purposes only; and use and reproduction, but not distribution, of adapted material for non-commercial purposes only, provided the original work is properly cited. |
spellingShingle | Bachtiar, Boy M. Fath, Turmidzi Widowati, Retno Bachtiar, Endang W. Quantification and Pathogenicity of Candida albicans in Denture-Wearing and Nondenture-Wearing Elderly |
title |
Quantification and Pathogenicity of
Candida albicans
in Denture-Wearing and Nondenture-Wearing Elderly
|
title_full |
Quantification and Pathogenicity of
Candida albicans
in Denture-Wearing and Nondenture-Wearing Elderly
|
title_fullStr |
Quantification and Pathogenicity of
Candida albicans
in Denture-Wearing and Nondenture-Wearing Elderly
|
title_full_unstemmed |
Quantification and Pathogenicity of
Candida albicans
in Denture-Wearing and Nondenture-Wearing Elderly
|
title_short |
Quantification and Pathogenicity of
Candida albicans
in Denture-Wearing and Nondenture-Wearing Elderly
|
title_sort | quantification and pathogenicity of
candida albicans
in denture-wearing and nondenture-wearing elderly |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7440952/ https://www.ncbi.nlm.nih.gov/pubmed/32542630 http://dx.doi.org/10.1055/s-0040-1712779 |
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