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Stability of MERS-CoV RNA on spin columns of RNA extraction kit at room temperature
Middle East respiratory syndrome coronavirus (MERS-CoV) is an emerging virus causing a highly fatal respiratory disease in humans. Confirmation of MERS-CoV infection and molecular study on the virus may require transportation of samples to specialized laboratories. While freezing at −80 °C is the go...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7441011/ https://www.ncbi.nlm.nih.gov/pubmed/32947111 http://dx.doi.org/10.1016/j.diagmicrobio.2020.115182 |
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author | Abdallah, Nermeen M.A. Zaki, Ali M. Abdel-Salam, Shimaa A. |
author_facet | Abdallah, Nermeen M.A. Zaki, Ali M. Abdel-Salam, Shimaa A. |
author_sort | Abdallah, Nermeen M.A. |
collection | PubMed |
description | Middle East respiratory syndrome coronavirus (MERS-CoV) is an emerging virus causing a highly fatal respiratory disease in humans. Confirmation of MERS-CoV infection and molecular study on the virus may require transportation of samples to specialized laboratories. While freezing at −80 °C is the gold standard method for RNA preservation, maintaining the integrity of viral RNA during transport will require additional precautions and, as a result, increase transport costs. We aimed at testing the stability of MERS-CoV RNA on spin columns of RNA extraction kit at room temperature for 16 weeks. Respiratory samples spiked with stock culture of MERS-CoV were extracted and loaded on QIAamp Viral RNA Mini Kit spin columns and preserved at room temperature. Amount of viral RNA was evaluated periodically by real-time quantitative reverse-transcription polymerase chain reaction. Minimal changes in cycle threshold values over the study period were noted, suggesting stability of viral RNA by this preservation method. |
format | Online Article Text |
id | pubmed-7441011 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-74410112020-08-21 Stability of MERS-CoV RNA on spin columns of RNA extraction kit at room temperature Abdallah, Nermeen M.A. Zaki, Ali M. Abdel-Salam, Shimaa A. Diagn Microbiol Infect Dis Virology Middle East respiratory syndrome coronavirus (MERS-CoV) is an emerging virus causing a highly fatal respiratory disease in humans. Confirmation of MERS-CoV infection and molecular study on the virus may require transportation of samples to specialized laboratories. While freezing at −80 °C is the gold standard method for RNA preservation, maintaining the integrity of viral RNA during transport will require additional precautions and, as a result, increase transport costs. We aimed at testing the stability of MERS-CoV RNA on spin columns of RNA extraction kit at room temperature for 16 weeks. Respiratory samples spiked with stock culture of MERS-CoV were extracted and loaded on QIAamp Viral RNA Mini Kit spin columns and preserved at room temperature. Amount of viral RNA was evaluated periodically by real-time quantitative reverse-transcription polymerase chain reaction. Minimal changes in cycle threshold values over the study period were noted, suggesting stability of viral RNA by this preservation method. Elsevier Inc. 2020-12 2020-08-21 /pmc/articles/PMC7441011/ /pubmed/32947111 http://dx.doi.org/10.1016/j.diagmicrobio.2020.115182 Text en © 2020 Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Virology Abdallah, Nermeen M.A. Zaki, Ali M. Abdel-Salam, Shimaa A. Stability of MERS-CoV RNA on spin columns of RNA extraction kit at room temperature |
title | Stability of MERS-CoV RNA on spin columns of RNA extraction kit at room temperature |
title_full | Stability of MERS-CoV RNA on spin columns of RNA extraction kit at room temperature |
title_fullStr | Stability of MERS-CoV RNA on spin columns of RNA extraction kit at room temperature |
title_full_unstemmed | Stability of MERS-CoV RNA on spin columns of RNA extraction kit at room temperature |
title_short | Stability of MERS-CoV RNA on spin columns of RNA extraction kit at room temperature |
title_sort | stability of mers-cov rna on spin columns of rna extraction kit at room temperature |
topic | Virology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7441011/ https://www.ncbi.nlm.nih.gov/pubmed/32947111 http://dx.doi.org/10.1016/j.diagmicrobio.2020.115182 |
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