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Generation of a recombinant chickenized monoclonal antibody against the neuraminidase of H9N2 avian influenza virus

We previously reported a monoclonal antibody (mAb), 1G8, against the neuraminidase (NA) of H9N2 avian influenza virus (AIV) with significant NA inhibitory activity. To generate a recombinant chickenized mAb (RCmAb) against the NA of H9N2 AIV for passive immunization in poultry, the gene of the fragm...

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Autores principales: Wang, Fei, Wang, Yajuan, Wan, Zhimin, Shao, Hongxia, Qian, Kun, Ye, Jianqiang, Qin, Aijian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7441100/
https://www.ncbi.nlm.nih.gov/pubmed/32816156
http://dx.doi.org/10.1186/s13568-020-01086-4
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author Wang, Fei
Wang, Yajuan
Wan, Zhimin
Shao, Hongxia
Qian, Kun
Ye, Jianqiang
Qin, Aijian
author_facet Wang, Fei
Wang, Yajuan
Wan, Zhimin
Shao, Hongxia
Qian, Kun
Ye, Jianqiang
Qin, Aijian
author_sort Wang, Fei
collection PubMed
description We previously reported a monoclonal antibody (mAb), 1G8, against the neuraminidase (NA) of H9N2 avian influenza virus (AIV) with significant NA inhibitory activity. To generate a recombinant chickenized mAb (RCmAb) against the NA of H9N2 AIV for passive immunization in poultry, the gene of the fragment of antigen binding (Fab) of mAb 1G8 was cloned and fused with the fragment crystallizable (Fc) gene of chicken IgY. The RCmAb 1G8 was expressed in COS-1 cells and could be detected in cell culture supernatant. The results of NA inhibitory activity tests of the RCmAb 1G8 in an enzyme-linked lectin assay (ELLA) and a microneutralization (MN) assay showed that the RCmAb 1G8 maintained significant NA inhibitory activity and neutralizing ability. This is the first chickenized antibody against AIV, which would be a good candidate for passive immunization in poultry.
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spelling pubmed-74411002020-08-28 Generation of a recombinant chickenized monoclonal antibody against the neuraminidase of H9N2 avian influenza virus Wang, Fei Wang, Yajuan Wan, Zhimin Shao, Hongxia Qian, Kun Ye, Jianqiang Qin, Aijian AMB Express Original Article We previously reported a monoclonal antibody (mAb), 1G8, against the neuraminidase (NA) of H9N2 avian influenza virus (AIV) with significant NA inhibitory activity. To generate a recombinant chickenized mAb (RCmAb) against the NA of H9N2 AIV for passive immunization in poultry, the gene of the fragment of antigen binding (Fab) of mAb 1G8 was cloned and fused with the fragment crystallizable (Fc) gene of chicken IgY. The RCmAb 1G8 was expressed in COS-1 cells and could be detected in cell culture supernatant. The results of NA inhibitory activity tests of the RCmAb 1G8 in an enzyme-linked lectin assay (ELLA) and a microneutralization (MN) assay showed that the RCmAb 1G8 maintained significant NA inhibitory activity and neutralizing ability. This is the first chickenized antibody against AIV, which would be a good candidate for passive immunization in poultry. Springer Berlin Heidelberg 2020-08-20 /pmc/articles/PMC7441100/ /pubmed/32816156 http://dx.doi.org/10.1186/s13568-020-01086-4 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Original Article
Wang, Fei
Wang, Yajuan
Wan, Zhimin
Shao, Hongxia
Qian, Kun
Ye, Jianqiang
Qin, Aijian
Generation of a recombinant chickenized monoclonal antibody against the neuraminidase of H9N2 avian influenza virus
title Generation of a recombinant chickenized monoclonal antibody against the neuraminidase of H9N2 avian influenza virus
title_full Generation of a recombinant chickenized monoclonal antibody against the neuraminidase of H9N2 avian influenza virus
title_fullStr Generation of a recombinant chickenized monoclonal antibody against the neuraminidase of H9N2 avian influenza virus
title_full_unstemmed Generation of a recombinant chickenized monoclonal antibody against the neuraminidase of H9N2 avian influenza virus
title_short Generation of a recombinant chickenized monoclonal antibody against the neuraminidase of H9N2 avian influenza virus
title_sort generation of a recombinant chickenized monoclonal antibody against the neuraminidase of h9n2 avian influenza virus
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7441100/
https://www.ncbi.nlm.nih.gov/pubmed/32816156
http://dx.doi.org/10.1186/s13568-020-01086-4
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