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Massively parallel single-molecule telomere length measurement with digital real-time PCR
Telomere length is a promising biomarker for age-associated diseases and cancer, but there are still substantial challenges to routine telomere analysis in clinics because of the lack of a simple and rapid yet scalable method for measurement. We developed the single telomere absolute-length rapid (S...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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American Association for the Advancement of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7442360/ https://www.ncbi.nlm.nih.gov/pubmed/32937369 http://dx.doi.org/10.1126/sciadv.abb7944 |
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author | Luo, Yongqiang Viswanathan, Ramya Hande, Manoor Prakash Loh, Amos Hong Pheng Cheow, Lih Feng |
author_facet | Luo, Yongqiang Viswanathan, Ramya Hande, Manoor Prakash Loh, Amos Hong Pheng Cheow, Lih Feng |
author_sort | Luo, Yongqiang |
collection | PubMed |
description | Telomere length is a promising biomarker for age-associated diseases and cancer, but there are still substantial challenges to routine telomere analysis in clinics because of the lack of a simple and rapid yet scalable method for measurement. We developed the single telomere absolute-length rapid (STAR) assay, a novel high-throughput digital real-time PCR approach for rapidly measuring the absolute lengths and quantities of individual telomere molecules. We show that this technique provides the accuracy and sensitivity to uncover associations between telomere length distribution and telomere maintenance mechanisms in cancer cell lines and primary tumors. The results indicate that the STAR assay is a powerful tool to enable the use of telomere length distribution as a biomarker in disease and population-wide studies. |
format | Online Article Text |
id | pubmed-7442360 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | American Association for the Advancement of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-74423602020-09-16 Massively parallel single-molecule telomere length measurement with digital real-time PCR Luo, Yongqiang Viswanathan, Ramya Hande, Manoor Prakash Loh, Amos Hong Pheng Cheow, Lih Feng Sci Adv Research Articles Telomere length is a promising biomarker for age-associated diseases and cancer, but there are still substantial challenges to routine telomere analysis in clinics because of the lack of a simple and rapid yet scalable method for measurement. We developed the single telomere absolute-length rapid (STAR) assay, a novel high-throughput digital real-time PCR approach for rapidly measuring the absolute lengths and quantities of individual telomere molecules. We show that this technique provides the accuracy and sensitivity to uncover associations between telomere length distribution and telomere maintenance mechanisms in cancer cell lines and primary tumors. The results indicate that the STAR assay is a powerful tool to enable the use of telomere length distribution as a biomarker in disease and population-wide studies. American Association for the Advancement of Science 2020-08-21 /pmc/articles/PMC7442360/ /pubmed/32937369 http://dx.doi.org/10.1126/sciadv.abb7944 Text en Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). https://creativecommons.org/licenses/by-nc/4.0/ https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (https://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited. |
spellingShingle | Research Articles Luo, Yongqiang Viswanathan, Ramya Hande, Manoor Prakash Loh, Amos Hong Pheng Cheow, Lih Feng Massively parallel single-molecule telomere length measurement with digital real-time PCR |
title | Massively parallel single-molecule telomere length measurement with digital real-time PCR |
title_full | Massively parallel single-molecule telomere length measurement with digital real-time PCR |
title_fullStr | Massively parallel single-molecule telomere length measurement with digital real-time PCR |
title_full_unstemmed | Massively parallel single-molecule telomere length measurement with digital real-time PCR |
title_short | Massively parallel single-molecule telomere length measurement with digital real-time PCR |
title_sort | massively parallel single-molecule telomere length measurement with digital real-time pcr |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7442360/ https://www.ncbi.nlm.nih.gov/pubmed/32937369 http://dx.doi.org/10.1126/sciadv.abb7944 |
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