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Assessment of function, histopathological changes, and oxidative stress in liver tissue due to ionizing and non-ionizing radiations

BACKGROUND: Compared to past decades, humans are exposed to rapidly increasing levels of radiofrequency electromagnetic radiations (RF-EMF). Despite numerous studies, the biological effects of human exposure to different levels of RF-EMF are not fully understood yet. This study aimed to evaluate the...

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Autores principales: Borzoueisileh, Sajad, Shabestani Monfared, Ali, Ghorbani, Hossein, Mortazavi, S. M. J., Zabihi, Ebrahim, Pouramir, Mehdi, Doustimotlagh, Amir Hossein, Shafiee, Mohsen, Niksirat, Fatemeh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Babol University of Medical Sciences 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7442457/
https://www.ncbi.nlm.nih.gov/pubmed/32874440
http://dx.doi.org/10.22088/cjim.11.3.315
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author Borzoueisileh, Sajad
Shabestani Monfared, Ali
Ghorbani, Hossein
Mortazavi, S. M. J.
Zabihi, Ebrahim
Pouramir, Mehdi
Doustimotlagh, Amir Hossein
Shafiee, Mohsen
Niksirat, Fatemeh
author_facet Borzoueisileh, Sajad
Shabestani Monfared, Ali
Ghorbani, Hossein
Mortazavi, S. M. J.
Zabihi, Ebrahim
Pouramir, Mehdi
Doustimotlagh, Amir Hossein
Shafiee, Mohsen
Niksirat, Fatemeh
author_sort Borzoueisileh, Sajad
collection PubMed
description BACKGROUND: Compared to past decades, humans are exposed to rapidly increasing levels of radiofrequency electromagnetic radiations (RF-EMF). Despite numerous studies, the biological effects of human exposure to different levels of RF-EMF are not fully understood yet. This study aimed to evaluate the bioeffects of exposure to "900/1800 MHz" and “2.4 GHz" RF-EMFs, and x-rays alone as well as their potential interactions, i.e. inducing simple additive, adaptive, or synergistic effects. METHODS: 120 Wistar rats were randomly divided into ten groups of 12 each. The rats were exposed to RF-EMF, 10 cGy, and 8 Gy x-rays, a combination of these exposures, or only sham-exposed. The levels of liver enzymes were determined in serum samples by an auto-analyzer. Moreover, the histopathological changes, and the levels of malondialdehyde (MDA), nitric oxide, ferric reducing antioxidant power, total thiols, and protein carbonyl (PCO) were measured. RESULTS: Among the markers of liver function, gamma-glutamyltransferase was not associated with irradiation but, aspartate transaminase, alanine transaminase, and alkaline phosphatase showed some levels of association. MDA and PCO levels after 8 Gy irradiation increased, but pre-exposure to RF-EMF could modulate their changes. At the cellular level, the frequency of lobular inflammation was associated with the type of intervention. CONCLUSION: The exposure to both ionizing and non-ionizing radiations could alter some liver function tests. A short term pre-exposure to RF-EMF before exposure to an 8 Gy challenging dose of x-rays caused the alterations in oxidative stress markers and liver function tests, which indicate that oxidative stress is possibly involved in the adaptive response.
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spelling pubmed-74424572020-08-31 Assessment of function, histopathological changes, and oxidative stress in liver tissue due to ionizing and non-ionizing radiations Borzoueisileh, Sajad Shabestani Monfared, Ali Ghorbani, Hossein Mortazavi, S. M. J. Zabihi, Ebrahim Pouramir, Mehdi Doustimotlagh, Amir Hossein Shafiee, Mohsen Niksirat, Fatemeh Caspian J Intern Med Original Article BACKGROUND: Compared to past decades, humans are exposed to rapidly increasing levels of radiofrequency electromagnetic radiations (RF-EMF). Despite numerous studies, the biological effects of human exposure to different levels of RF-EMF are not fully understood yet. This study aimed to evaluate the bioeffects of exposure to "900/1800 MHz" and “2.4 GHz" RF-EMFs, and x-rays alone as well as their potential interactions, i.e. inducing simple additive, adaptive, or synergistic effects. METHODS: 120 Wistar rats were randomly divided into ten groups of 12 each. The rats were exposed to RF-EMF, 10 cGy, and 8 Gy x-rays, a combination of these exposures, or only sham-exposed. The levels of liver enzymes were determined in serum samples by an auto-analyzer. Moreover, the histopathological changes, and the levels of malondialdehyde (MDA), nitric oxide, ferric reducing antioxidant power, total thiols, and protein carbonyl (PCO) were measured. RESULTS: Among the markers of liver function, gamma-glutamyltransferase was not associated with irradiation but, aspartate transaminase, alanine transaminase, and alkaline phosphatase showed some levels of association. MDA and PCO levels after 8 Gy irradiation increased, but pre-exposure to RF-EMF could modulate their changes. At the cellular level, the frequency of lobular inflammation was associated with the type of intervention. CONCLUSION: The exposure to both ionizing and non-ionizing radiations could alter some liver function tests. A short term pre-exposure to RF-EMF before exposure to an 8 Gy challenging dose of x-rays caused the alterations in oxidative stress markers and liver function tests, which indicate that oxidative stress is possibly involved in the adaptive response. Babol University of Medical Sciences 2020-05 /pmc/articles/PMC7442457/ /pubmed/32874440 http://dx.doi.org/10.22088/cjim.11.3.315 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Borzoueisileh, Sajad
Shabestani Monfared, Ali
Ghorbani, Hossein
Mortazavi, S. M. J.
Zabihi, Ebrahim
Pouramir, Mehdi
Doustimotlagh, Amir Hossein
Shafiee, Mohsen
Niksirat, Fatemeh
Assessment of function, histopathological changes, and oxidative stress in liver tissue due to ionizing and non-ionizing radiations
title Assessment of function, histopathological changes, and oxidative stress in liver tissue due to ionizing and non-ionizing radiations
title_full Assessment of function, histopathological changes, and oxidative stress in liver tissue due to ionizing and non-ionizing radiations
title_fullStr Assessment of function, histopathological changes, and oxidative stress in liver tissue due to ionizing and non-ionizing radiations
title_full_unstemmed Assessment of function, histopathological changes, and oxidative stress in liver tissue due to ionizing and non-ionizing radiations
title_short Assessment of function, histopathological changes, and oxidative stress in liver tissue due to ionizing and non-ionizing radiations
title_sort assessment of function, histopathological changes, and oxidative stress in liver tissue due to ionizing and non-ionizing radiations
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7442457/
https://www.ncbi.nlm.nih.gov/pubmed/32874440
http://dx.doi.org/10.22088/cjim.11.3.315
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