Discovery of small-molecule enzyme activators by activity-based protein profiling

Activity-based protein profiling (ABPP) has been used extensively to discover and optimize selective inhibitors of enzymes. Here, we show that ABPP can also be implemented to identify the converse – small-molecule enzyme activators. Using a kinetically controlled, fluorescence polarization-ABPP assa...

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Detalles Bibliográficos
Autores principales: Kok, Bernard P., Ghimire, Srijana, Kim, Woojoo, Chatterjee, Shreyosree, Johns, Tyler, Kitamura, Seiya, Eberhardt, Jerome, Ogasawara, Daisuke, Xu, Janice, Sukiasyan, Ara, Kim, Sean M., Godio, Cristina, Bittencourt, Julia M., Cameron, Michael, Galmozzi, Andrea, Forli, Stefano, Wolan, Dennis W., Cravatt, Benjamin F., Boger, Dale L., Saez, Enrique
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7442688/
https://www.ncbi.nlm.nih.gov/pubmed/32514184
http://dx.doi.org/10.1038/s41589-020-0555-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7442688/
https://www.ncbi.nlm.nih.gov/pubmed/32514184
http://dx.doi.org/10.1038/s41589-020-0555-4

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