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Total reflection X-ray fluorescence spectrometry for trace determination of iron and some additional elements in biological samples

Trace elements are essential for life and their concentration in cells and tissues must be tightly maintained and controlled to avoid pathological conditions. Established methods to measure the concentration of trace elements in biological matrices often provide only single element information, are...

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Autores principales: Gruber, Andreas, Müller, Riccarda, Wagner, Alessa, Colucci, Silvia, Spasić, Maja Vujić, Leopold, Kerstin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7442763/
https://www.ncbi.nlm.nih.gov/pubmed/32337622
http://dx.doi.org/10.1007/s00216-020-02614-8
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author Gruber, Andreas
Müller, Riccarda
Wagner, Alessa
Colucci, Silvia
Spasić, Maja Vujić
Leopold, Kerstin
author_facet Gruber, Andreas
Müller, Riccarda
Wagner, Alessa
Colucci, Silvia
Spasić, Maja Vujić
Leopold, Kerstin
author_sort Gruber, Andreas
collection PubMed
description Trace elements are essential for life and their concentration in cells and tissues must be tightly maintained and controlled to avoid pathological conditions. Established methods to measure the concentration of trace elements in biological matrices often provide only single element information, are time-consuming, and require special sample preparation. Therefore, the development of straightforward and rapid analytical methods for enhanced, multi-trace element determination in biological samples is an important and raising field of trace element analysis. Herein, we report on the development and validation of a reliable method based on total reflection X-ray fluorescence (TXRF) analysis to precisely quantify iron and other trace metals in a variety of biological samples, such as the liver, parenchymal and non-parenchymal liver cells, and bone marrow–derived macrophages. We show that TXRF allows fast and simple one-point calibration by addition of an internal standard and has the potential of multi-element analysis in minute sample amounts. The method was validated for iron by recovery experiments in homogenates in a wide concentration range from 1 to 1600 μg/L applying well-established graphite furnace atomic absorption spectrometry (GFAAS) as a reference method. The recovery rate of 99.93 ± 0.14% reveals the absence of systematic errors. Furthermore, the standard reference material “bovine liver” (SRM 1577c, NIST) was investigated in order to validate the method for further biometals. Quantitative recoveries (92–106%) of copper, iron, zinc, and manganese prove the suitability of the developed method. The limits of detection for the minute sample amounts are in the low picogram range. [Figure: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00216-020-02614-8) contains supplementary material, which is available to authorized users.
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spelling pubmed-74427632020-08-27 Total reflection X-ray fluorescence spectrometry for trace determination of iron and some additional elements in biological samples Gruber, Andreas Müller, Riccarda Wagner, Alessa Colucci, Silvia Spasić, Maja Vujić Leopold, Kerstin Anal Bioanal Chem Research Paper Trace elements are essential for life and their concentration in cells and tissues must be tightly maintained and controlled to avoid pathological conditions. Established methods to measure the concentration of trace elements in biological matrices often provide only single element information, are time-consuming, and require special sample preparation. Therefore, the development of straightforward and rapid analytical methods for enhanced, multi-trace element determination in biological samples is an important and raising field of trace element analysis. Herein, we report on the development and validation of a reliable method based on total reflection X-ray fluorescence (TXRF) analysis to precisely quantify iron and other trace metals in a variety of biological samples, such as the liver, parenchymal and non-parenchymal liver cells, and bone marrow–derived macrophages. We show that TXRF allows fast and simple one-point calibration by addition of an internal standard and has the potential of multi-element analysis in minute sample amounts. The method was validated for iron by recovery experiments in homogenates in a wide concentration range from 1 to 1600 μg/L applying well-established graphite furnace atomic absorption spectrometry (GFAAS) as a reference method. The recovery rate of 99.93 ± 0.14% reveals the absence of systematic errors. Furthermore, the standard reference material “bovine liver” (SRM 1577c, NIST) was investigated in order to validate the method for further biometals. Quantitative recoveries (92–106%) of copper, iron, zinc, and manganese prove the suitability of the developed method. The limits of detection for the minute sample amounts are in the low picogram range. [Figure: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00216-020-02614-8) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2020-04-26 2020 /pmc/articles/PMC7442763/ /pubmed/32337622 http://dx.doi.org/10.1007/s00216-020-02614-8 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Paper
Gruber, Andreas
Müller, Riccarda
Wagner, Alessa
Colucci, Silvia
Spasić, Maja Vujić
Leopold, Kerstin
Total reflection X-ray fluorescence spectrometry for trace determination of iron and some additional elements in biological samples
title Total reflection X-ray fluorescence spectrometry for trace determination of iron and some additional elements in biological samples
title_full Total reflection X-ray fluorescence spectrometry for trace determination of iron and some additional elements in biological samples
title_fullStr Total reflection X-ray fluorescence spectrometry for trace determination of iron and some additional elements in biological samples
title_full_unstemmed Total reflection X-ray fluorescence spectrometry for trace determination of iron and some additional elements in biological samples
title_short Total reflection X-ray fluorescence spectrometry for trace determination of iron and some additional elements in biological samples
title_sort total reflection x-ray fluorescence spectrometry for trace determination of iron and some additional elements in biological samples
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7442763/
https://www.ncbi.nlm.nih.gov/pubmed/32337622
http://dx.doi.org/10.1007/s00216-020-02614-8
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