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NIR-II bioluminescence for in vivo high contrast imaging and in situ ATP-mediated metastases tracing

Bioluminescence imaging has been widely used in life sciences and biomedical applications. However, conventional bioluminescence imaging usually operates in the visible region, which hampers the high-performance in vivo optical imaging due to the strong tissue absorption and scattering. To address t...

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Autores principales: Lu, Lingfei, Li, Benhao, Ding, Suwan, Fan, Yong, Wang, Shangfeng, Sun, Caixia, Zhao, Mengyao, Zhao, Chun-Xia, Zhang, Fan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7442788/
https://www.ncbi.nlm.nih.gov/pubmed/32826886
http://dx.doi.org/10.1038/s41467-020-18051-1
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author Lu, Lingfei
Li, Benhao
Ding, Suwan
Fan, Yong
Wang, Shangfeng
Sun, Caixia
Zhao, Mengyao
Zhao, Chun-Xia
Zhang, Fan
author_facet Lu, Lingfei
Li, Benhao
Ding, Suwan
Fan, Yong
Wang, Shangfeng
Sun, Caixia
Zhao, Mengyao
Zhao, Chun-Xia
Zhang, Fan
author_sort Lu, Lingfei
collection PubMed
description Bioluminescence imaging has been widely used in life sciences and biomedical applications. However, conventional bioluminescence imaging usually operates in the visible region, which hampers the high-performance in vivo optical imaging due to the strong tissue absorption and scattering. To address this challenge, here we present bioluminescence probes (BPs) with emission in the second near infrared (NIR-II) region at 1029 nm by employing bioluminescence resonance energy transfer (BRET) and two-step fluorescence resonance energy transfer (FRET) with a specially designed cyanine dye FD-1029. The biocompatible NIR-II-BPs are successfully applied to vessels and lymphatics imaging in mice, which gives ~5 times higher signal-to-noise ratios and ~1.5 times higher spatial resolution than those obtained by NIR-II fluorescence imaging and conventional bioluminescence imaging. Their capability of multiplexed imaging is also well displayed. Taking advantage of the ATP-responding character, the NIR-II-BPs are able to recognize tumor metastasis with a high tumor-to-normal tissue ratio at 83.4.
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spelling pubmed-74427882020-09-02 NIR-II bioluminescence for in vivo high contrast imaging and in situ ATP-mediated metastases tracing Lu, Lingfei Li, Benhao Ding, Suwan Fan, Yong Wang, Shangfeng Sun, Caixia Zhao, Mengyao Zhao, Chun-Xia Zhang, Fan Nat Commun Article Bioluminescence imaging has been widely used in life sciences and biomedical applications. However, conventional bioluminescence imaging usually operates in the visible region, which hampers the high-performance in vivo optical imaging due to the strong tissue absorption and scattering. To address this challenge, here we present bioluminescence probes (BPs) with emission in the second near infrared (NIR-II) region at 1029 nm by employing bioluminescence resonance energy transfer (BRET) and two-step fluorescence resonance energy transfer (FRET) with a specially designed cyanine dye FD-1029. The biocompatible NIR-II-BPs are successfully applied to vessels and lymphatics imaging in mice, which gives ~5 times higher signal-to-noise ratios and ~1.5 times higher spatial resolution than those obtained by NIR-II fluorescence imaging and conventional bioluminescence imaging. Their capability of multiplexed imaging is also well displayed. Taking advantage of the ATP-responding character, the NIR-II-BPs are able to recognize tumor metastasis with a high tumor-to-normal tissue ratio at 83.4. Nature Publishing Group UK 2020-08-21 /pmc/articles/PMC7442788/ /pubmed/32826886 http://dx.doi.org/10.1038/s41467-020-18051-1 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Lu, Lingfei
Li, Benhao
Ding, Suwan
Fan, Yong
Wang, Shangfeng
Sun, Caixia
Zhao, Mengyao
Zhao, Chun-Xia
Zhang, Fan
NIR-II bioluminescence for in vivo high contrast imaging and in situ ATP-mediated metastases tracing
title NIR-II bioluminescence for in vivo high contrast imaging and in situ ATP-mediated metastases tracing
title_full NIR-II bioluminescence for in vivo high contrast imaging and in situ ATP-mediated metastases tracing
title_fullStr NIR-II bioluminescence for in vivo high contrast imaging and in situ ATP-mediated metastases tracing
title_full_unstemmed NIR-II bioluminescence for in vivo high contrast imaging and in situ ATP-mediated metastases tracing
title_short NIR-II bioluminescence for in vivo high contrast imaging and in situ ATP-mediated metastases tracing
title_sort nir-ii bioluminescence for in vivo high contrast imaging and in situ atp-mediated metastases tracing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7442788/
https://www.ncbi.nlm.nih.gov/pubmed/32826886
http://dx.doi.org/10.1038/s41467-020-18051-1
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