Cargando…
ALV-J inhibits autophagy through the GADD45β/MEKK4/P38MAPK signaling pathway and mediates apoptosis following autophagy
Autophagy and apoptosis, which are important processes for host immunity, are commonly exploited by viruses to facilitate their survival. However, to the best of our knowledge, very few studies have researched the mechanisms of action of the autophagic and apoptotic signaling pathways following vira...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7442830/ https://www.ncbi.nlm.nih.gov/pubmed/32826872 http://dx.doi.org/10.1038/s41419-020-02841-y |
_version_ | 1783573513848750080 |
---|---|
author | Liao, Zhihong Zhang, Xinheng Song, Cailiang Lin, Wencheng Cheng, Yuzhen Xie, Zi Chen, Sheng Nie, Yu Li, Aijun Zhang, Huanmin Li, Hongxin Li, Haiyun Xie, Qingmei |
author_facet | Liao, Zhihong Zhang, Xinheng Song, Cailiang Lin, Wencheng Cheng, Yuzhen Xie, Zi Chen, Sheng Nie, Yu Li, Aijun Zhang, Huanmin Li, Hongxin Li, Haiyun Xie, Qingmei |
author_sort | Liao, Zhihong |
collection | PubMed |
description | Autophagy and apoptosis, which are important processes for host immunity, are commonly exploited by viruses to facilitate their survival. However, to the best of our knowledge, very few studies have researched the mechanisms of action of the autophagic and apoptotic signaling pathways following viral infection. Thus, the present study aimed to investigate the mechanisms of action of growth arrest and DNA-damage-inducible β (GADD45β), an important resistance gene involved in the host resistance to ALV-J. Both ALV-J infection and the overexpression of GADD45β inhibited autophagy during the early stages, which prevented the autophagosomes from binding to the lysosomes and resulted in an incomplete autophagic flux. Notably, GADD45β was discovered to interact with MEKK4 in DF-1 cells. The genetic knockdown of GADD45β and MEKK4 using small interfering RNA-affected ALV-J infection, which suggested that ALV-J may promote the binding of GADD45β to MEKK4 to activate the p38MAPK signaling pathway, which subsequently inhibits autophagy. Furthermore, ALV-J was revealed to affect the autophagic pathway prior to affecting the apoptotic pathway. In conclusion, to the best of our knowledge, the present study was the first to investigate the combined effects of ALV-J infection on autophagy and apoptosis, and to suggest that ALV-J inhibits autophagy via the GADD45β/MEKK4/p38MAPK signaling pathway. |
format | Online Article Text |
id | pubmed-7442830 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-74428302020-09-02 ALV-J inhibits autophagy through the GADD45β/MEKK4/P38MAPK signaling pathway and mediates apoptosis following autophagy Liao, Zhihong Zhang, Xinheng Song, Cailiang Lin, Wencheng Cheng, Yuzhen Xie, Zi Chen, Sheng Nie, Yu Li, Aijun Zhang, Huanmin Li, Hongxin Li, Haiyun Xie, Qingmei Cell Death Dis Article Autophagy and apoptosis, which are important processes for host immunity, are commonly exploited by viruses to facilitate their survival. However, to the best of our knowledge, very few studies have researched the mechanisms of action of the autophagic and apoptotic signaling pathways following viral infection. Thus, the present study aimed to investigate the mechanisms of action of growth arrest and DNA-damage-inducible β (GADD45β), an important resistance gene involved in the host resistance to ALV-J. Both ALV-J infection and the overexpression of GADD45β inhibited autophagy during the early stages, which prevented the autophagosomes from binding to the lysosomes and resulted in an incomplete autophagic flux. Notably, GADD45β was discovered to interact with MEKK4 in DF-1 cells. The genetic knockdown of GADD45β and MEKK4 using small interfering RNA-affected ALV-J infection, which suggested that ALV-J may promote the binding of GADD45β to MEKK4 to activate the p38MAPK signaling pathway, which subsequently inhibits autophagy. Furthermore, ALV-J was revealed to affect the autophagic pathway prior to affecting the apoptotic pathway. In conclusion, to the best of our knowledge, the present study was the first to investigate the combined effects of ALV-J infection on autophagy and apoptosis, and to suggest that ALV-J inhibits autophagy via the GADD45β/MEKK4/p38MAPK signaling pathway. Nature Publishing Group UK 2020-08-12 /pmc/articles/PMC7442830/ /pubmed/32826872 http://dx.doi.org/10.1038/s41419-020-02841-y Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Liao, Zhihong Zhang, Xinheng Song, Cailiang Lin, Wencheng Cheng, Yuzhen Xie, Zi Chen, Sheng Nie, Yu Li, Aijun Zhang, Huanmin Li, Hongxin Li, Haiyun Xie, Qingmei ALV-J inhibits autophagy through the GADD45β/MEKK4/P38MAPK signaling pathway and mediates apoptosis following autophagy |
title | ALV-J inhibits autophagy through the GADD45β/MEKK4/P38MAPK signaling pathway and mediates apoptosis following autophagy |
title_full | ALV-J inhibits autophagy through the GADD45β/MEKK4/P38MAPK signaling pathway and mediates apoptosis following autophagy |
title_fullStr | ALV-J inhibits autophagy through the GADD45β/MEKK4/P38MAPK signaling pathway and mediates apoptosis following autophagy |
title_full_unstemmed | ALV-J inhibits autophagy through the GADD45β/MEKK4/P38MAPK signaling pathway and mediates apoptosis following autophagy |
title_short | ALV-J inhibits autophagy through the GADD45β/MEKK4/P38MAPK signaling pathway and mediates apoptosis following autophagy |
title_sort | alv-j inhibits autophagy through the gadd45β/mekk4/p38mapk signaling pathway and mediates apoptosis following autophagy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7442830/ https://www.ncbi.nlm.nih.gov/pubmed/32826872 http://dx.doi.org/10.1038/s41419-020-02841-y |
work_keys_str_mv | AT liaozhihong alvjinhibitsautophagythroughthegadd45bmekk4p38mapksignalingpathwayandmediatesapoptosisfollowingautophagy AT zhangxinheng alvjinhibitsautophagythroughthegadd45bmekk4p38mapksignalingpathwayandmediatesapoptosisfollowingautophagy AT songcailiang alvjinhibitsautophagythroughthegadd45bmekk4p38mapksignalingpathwayandmediatesapoptosisfollowingautophagy AT linwencheng alvjinhibitsautophagythroughthegadd45bmekk4p38mapksignalingpathwayandmediatesapoptosisfollowingautophagy AT chengyuzhen alvjinhibitsautophagythroughthegadd45bmekk4p38mapksignalingpathwayandmediatesapoptosisfollowingautophagy AT xiezi alvjinhibitsautophagythroughthegadd45bmekk4p38mapksignalingpathwayandmediatesapoptosisfollowingautophagy AT chensheng alvjinhibitsautophagythroughthegadd45bmekk4p38mapksignalingpathwayandmediatesapoptosisfollowingautophagy AT nieyu alvjinhibitsautophagythroughthegadd45bmekk4p38mapksignalingpathwayandmediatesapoptosisfollowingautophagy AT liaijun alvjinhibitsautophagythroughthegadd45bmekk4p38mapksignalingpathwayandmediatesapoptosisfollowingautophagy AT zhanghuanmin alvjinhibitsautophagythroughthegadd45bmekk4p38mapksignalingpathwayandmediatesapoptosisfollowingautophagy AT lihongxin alvjinhibitsautophagythroughthegadd45bmekk4p38mapksignalingpathwayandmediatesapoptosisfollowingautophagy AT lihaiyun alvjinhibitsautophagythroughthegadd45bmekk4p38mapksignalingpathwayandmediatesapoptosisfollowingautophagy AT xieqingmei alvjinhibitsautophagythroughthegadd45bmekk4p38mapksignalingpathwayandmediatesapoptosisfollowingautophagy |