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Systematic analysis of disease‐specific immunological signatures in patients with febrile illness from Saudi Arabia

OBJECTIVES: Little is known about the prevalence of febrile illness in the Arabian region as clinical, laboratory and immunological profiling remains largely uncharacterised. METHODS: A total of 2018 febrile patients from Jazan, Saudi Arabia, were recruited between 2014 and 2017. Patients were scree...

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Autores principales: Kam, Yiu‐Wing, Ahmed, Mohamed Yousif, Amrun, Siti Naqiah, Lee, Bernett, Refaie, Tarik, Elgizouli, Kamla, Fong, Siew‐Wai, Renia, Laurent, Ng, Lisa FP
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7443187/
https://www.ncbi.nlm.nih.gov/pubmed/32864128
http://dx.doi.org/10.1002/cti2.1163
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author Kam, Yiu‐Wing
Ahmed, Mohamed Yousif
Amrun, Siti Naqiah
Lee, Bernett
Refaie, Tarik
Elgizouli, Kamla
Fong, Siew‐Wai
Renia, Laurent
Ng, Lisa FP
author_facet Kam, Yiu‐Wing
Ahmed, Mohamed Yousif
Amrun, Siti Naqiah
Lee, Bernett
Refaie, Tarik
Elgizouli, Kamla
Fong, Siew‐Wai
Renia, Laurent
Ng, Lisa FP
author_sort Kam, Yiu‐Wing
collection PubMed
description OBJECTIVES: Little is known about the prevalence of febrile illness in the Arabian region as clinical, laboratory and immunological profiling remains largely uncharacterised. METHODS: A total of 2018 febrile patients from Jazan, Saudi Arabia, were recruited between 2014 and 2017. Patients were screened for dengue and chikungunya virus, Plasmodium, Brucella, Neisseria meningitidis, group A streptococcus and Leptospira. Clinical history and biochemical parameters from blood tests were collected. Patient sera of selected disease‐confirmed infections were quantified for immune mediators by multiplex microbead‐based immunoassays. RESULTS: Approximately 20% of febrile patients were tested positive for one of the pathogens, and they presented overlapping clinical and laboratory parameters. Nonetheless, eight disease‐specific immune mediators were identified as potential biomarkers for dengue (MIP‐1α, MCP‐1), malaria (TNF‐α), streptococcal and meningococcal (eotaxin, GRO‐α, RANTES, SDF‐1α and PIGF‐1) infections, with high specificity and sensitivity profiles. Notably, based on the conditional inference model, six of these mediators (MIP‐1α, TNF‐α, GRO‐α, RANTES, SDF‐1α and PIGF‐1) were revealed to be 68.4% accurate in diagnosing different febrile infections, including those of unknown diseases. CONCLUSIONS: This study is the first extensive characterisation of the clinical analysis and immune biomarkers of several clinically important febrile infections in Saudi Arabia. Importantly, an immune signature with robust accuracy, specificity and sensitivity in differentiating several febrile infections was identified, providing useful insights into patient disease management in the Arabian Peninsula.
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spelling pubmed-74431872020-08-28 Systematic analysis of disease‐specific immunological signatures in patients with febrile illness from Saudi Arabia Kam, Yiu‐Wing Ahmed, Mohamed Yousif Amrun, Siti Naqiah Lee, Bernett Refaie, Tarik Elgizouli, Kamla Fong, Siew‐Wai Renia, Laurent Ng, Lisa FP Clin Transl Immunology Original Article OBJECTIVES: Little is known about the prevalence of febrile illness in the Arabian region as clinical, laboratory and immunological profiling remains largely uncharacterised. METHODS: A total of 2018 febrile patients from Jazan, Saudi Arabia, were recruited between 2014 and 2017. Patients were screened for dengue and chikungunya virus, Plasmodium, Brucella, Neisseria meningitidis, group A streptococcus and Leptospira. Clinical history and biochemical parameters from blood tests were collected. Patient sera of selected disease‐confirmed infections were quantified for immune mediators by multiplex microbead‐based immunoassays. RESULTS: Approximately 20% of febrile patients were tested positive for one of the pathogens, and they presented overlapping clinical and laboratory parameters. Nonetheless, eight disease‐specific immune mediators were identified as potential biomarkers for dengue (MIP‐1α, MCP‐1), malaria (TNF‐α), streptococcal and meningococcal (eotaxin, GRO‐α, RANTES, SDF‐1α and PIGF‐1) infections, with high specificity and sensitivity profiles. Notably, based on the conditional inference model, six of these mediators (MIP‐1α, TNF‐α, GRO‐α, RANTES, SDF‐1α and PIGF‐1) were revealed to be 68.4% accurate in diagnosing different febrile infections, including those of unknown diseases. CONCLUSIONS: This study is the first extensive characterisation of the clinical analysis and immune biomarkers of several clinically important febrile infections in Saudi Arabia. Importantly, an immune signature with robust accuracy, specificity and sensitivity in differentiating several febrile infections was identified, providing useful insights into patient disease management in the Arabian Peninsula. John Wiley and Sons Inc. 2020-08-22 /pmc/articles/PMC7443187/ /pubmed/32864128 http://dx.doi.org/10.1002/cti2.1163 Text en © 2020 The Authors. Clinical & Translational Immunology published by John Wiley & Sons Australia, Ltd on behalf of Australian New Zealand Society for Immunology, Inc. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/3.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Original Article
Kam, Yiu‐Wing
Ahmed, Mohamed Yousif
Amrun, Siti Naqiah
Lee, Bernett
Refaie, Tarik
Elgizouli, Kamla
Fong, Siew‐Wai
Renia, Laurent
Ng, Lisa FP
Systematic analysis of disease‐specific immunological signatures in patients with febrile illness from Saudi Arabia
title Systematic analysis of disease‐specific immunological signatures in patients with febrile illness from Saudi Arabia
title_full Systematic analysis of disease‐specific immunological signatures in patients with febrile illness from Saudi Arabia
title_fullStr Systematic analysis of disease‐specific immunological signatures in patients with febrile illness from Saudi Arabia
title_full_unstemmed Systematic analysis of disease‐specific immunological signatures in patients with febrile illness from Saudi Arabia
title_short Systematic analysis of disease‐specific immunological signatures in patients with febrile illness from Saudi Arabia
title_sort systematic analysis of disease‐specific immunological signatures in patients with febrile illness from saudi arabia
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7443187/
https://www.ncbi.nlm.nih.gov/pubmed/32864128
http://dx.doi.org/10.1002/cti2.1163
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