Cargando…
Dorsal Horn of Mouse Lumbar Spinal Cord Imaged with CLARITY
The organization of the mouse spinal dorsal horn has been delineated in 2D for the six Rexed laminae in our publication Atlas of the Spinal Cord: Mouse, Rat, Rhesus, Marmoset, and Human. In the present study, the tissue clearing technique CLARITY was used to observe the cyto- and chemoarchitecture o...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7443243/ https://www.ncbi.nlm.nih.gov/pubmed/32855963 http://dx.doi.org/10.1155/2020/3689380 |
_version_ | 1783573595373436928 |
---|---|
author | Sengul, Gulgun Liang, Huazheng Furlong, Teri M. Paxinos, George |
author_facet | Sengul, Gulgun Liang, Huazheng Furlong, Teri M. Paxinos, George |
author_sort | Sengul, Gulgun |
collection | PubMed |
description | The organization of the mouse spinal dorsal horn has been delineated in 2D for the six Rexed laminae in our publication Atlas of the Spinal Cord: Mouse, Rat, Rhesus, Marmoset, and Human. In the present study, the tissue clearing technique CLARITY was used to observe the cyto- and chemoarchitecture of the mouse spinal cord in 3D, using a variety of immunohistochemical markers. We confirm prior observations regarding the location of glycine and serotonin immunoreactivities. Novel observations include the demonstration of numerous calcitonin gene-related peptide (CGRP) perikarya, as well as CGRP fibers and terminals in all laminae of the dorsal horn. We also observed sparse choline acetyltransferase (ChAT) immunoreactivity in small perikarya and fibers and terminals in all dorsal horn laminae, while gamma aminobutyric acid (GABA) and glutamate decarboxylase-67 (GAD67) immunoreactivities were found only in small perikarya and fibers. Finally, numerous serotonergic fibers were observed in all laminae of the dorsal horn. In conclusion, CLARITY confirmed the 2D immunohistochemical properties of the spinal cord. Furthermore, we observed novel anatomical characteristics of the spinal cord and demonstrated that CLARITY can be used on spinal cord tissue to examine many proteins of interest. |
format | Online Article Text |
id | pubmed-7443243 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-74432432020-08-26 Dorsal Horn of Mouse Lumbar Spinal Cord Imaged with CLARITY Sengul, Gulgun Liang, Huazheng Furlong, Teri M. Paxinos, George Biomed Res Int Research Article The organization of the mouse spinal dorsal horn has been delineated in 2D for the six Rexed laminae in our publication Atlas of the Spinal Cord: Mouse, Rat, Rhesus, Marmoset, and Human. In the present study, the tissue clearing technique CLARITY was used to observe the cyto- and chemoarchitecture of the mouse spinal cord in 3D, using a variety of immunohistochemical markers. We confirm prior observations regarding the location of glycine and serotonin immunoreactivities. Novel observations include the demonstration of numerous calcitonin gene-related peptide (CGRP) perikarya, as well as CGRP fibers and terminals in all laminae of the dorsal horn. We also observed sparse choline acetyltransferase (ChAT) immunoreactivity in small perikarya and fibers and terminals in all dorsal horn laminae, while gamma aminobutyric acid (GABA) and glutamate decarboxylase-67 (GAD67) immunoreactivities were found only in small perikarya and fibers. Finally, numerous serotonergic fibers were observed in all laminae of the dorsal horn. In conclusion, CLARITY confirmed the 2D immunohistochemical properties of the spinal cord. Furthermore, we observed novel anatomical characteristics of the spinal cord and demonstrated that CLARITY can be used on spinal cord tissue to examine many proteins of interest. Hindawi 2020-08-14 /pmc/articles/PMC7443243/ /pubmed/32855963 http://dx.doi.org/10.1155/2020/3689380 Text en Copyright © 2020 Gulgun Sengul et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Sengul, Gulgun Liang, Huazheng Furlong, Teri M. Paxinos, George Dorsal Horn of Mouse Lumbar Spinal Cord Imaged with CLARITY |
title | Dorsal Horn of Mouse Lumbar Spinal Cord Imaged with CLARITY |
title_full | Dorsal Horn of Mouse Lumbar Spinal Cord Imaged with CLARITY |
title_fullStr | Dorsal Horn of Mouse Lumbar Spinal Cord Imaged with CLARITY |
title_full_unstemmed | Dorsal Horn of Mouse Lumbar Spinal Cord Imaged with CLARITY |
title_short | Dorsal Horn of Mouse Lumbar Spinal Cord Imaged with CLARITY |
title_sort | dorsal horn of mouse lumbar spinal cord imaged with clarity |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7443243/ https://www.ncbi.nlm.nih.gov/pubmed/32855963 http://dx.doi.org/10.1155/2020/3689380 |
work_keys_str_mv | AT sengulgulgun dorsalhornofmouselumbarspinalcordimagedwithclarity AT lianghuazheng dorsalhornofmouselumbarspinalcordimagedwithclarity AT furlongterim dorsalhornofmouselumbarspinalcordimagedwithclarity AT paxinosgeorge dorsalhornofmouselumbarspinalcordimagedwithclarity |