Cargando…
Simplified detection of polyhistidine-tagged proteins in gels and membranes using a UV-excitable dye and a multiple chelator head pair
The polyhistidine tag (His-tag) is one of the most popular protein tags used in the life sciences. Traditionally, the detection of His-tagged proteins relies on immunoblotting with anti-His antibodies. This approach is laborious for certain applications, such as protein purification, where time and...
Autores principales: | Raducanu, Vlad-Stefan, Isaioglou, Ioannis, Raducanu, Daniela-Violeta, Merzaban, Jasmeen S., Hamdan, Samir M. |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Biochemistry and Molecular Biology
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7443479/ https://www.ncbi.nlm.nih.gov/pubmed/32647010 http://dx.doi.org/10.1074/jbc.RA120.014132 |
Ejemplares similares
-
TSGIT: An N‐ and C‐terminal tandem tag system for purification of native and intein‐mediated ligation‐ready proteins
por: Raducanu, Vlad‐Stefan, et al.
Publicado: (2020) -
Initial state of DNA-Dye complex sets the stage for protein induced fluorescence modulation
por: Rashid, Fahad, et al.
Publicado: (2019) -
Impact of an N-terminal
Polyhistidine Tag on
Protein Thermal Stability
por: Booth, William T., et al.
Publicado: (2018) -
Surface-Attached Polyhistidine-Tag Proteins Characterized by FTIR Difference Spectroscopy
por: Pinkerneil, Philipp, et al.
Publicado: (2012) -
Nickel (II) and Cobalt (II) Alginate Biopolymers as a “Carry and Release” Platform for Polyhistidine-Tagged Proteins
por: Dumitrașcu, Andrei-Mihai, et al.
Publicado: (2022)