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Diagnosis and prevalence of two new species of haplosporidians infecting shore crabs Carcinus maenas: Haplosporidium carcini n. sp., and H. cranc n. sp.

This study provides a morphological and phylogenetic characterization of two novel species of the order Haplosporida (Haplosporidium carcini n. sp., and H. cranc n. sp.) infecting the common shore crab Carcinus maenas collected at one location in Swansea Bay, South Wales, UK. Both parasites were obs...

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Autores principales: Davies, Charlotte E., Bass, David, Ward, Georgia M., Batista, Frederico M., Malkin, Sophie H., Thomas, Jessica E., Bateman, Kelly, Feist, Stephen W., Coates, Christopher J., Rowley, Andrew F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cambridge University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7443749/
https://www.ncbi.nlm.nih.gov/pubmed/32539882
http://dx.doi.org/10.1017/S0031182020000980
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author Davies, Charlotte E.
Bass, David
Ward, Georgia M.
Batista, Frederico M.
Malkin, Sophie H.
Thomas, Jessica E.
Bateman, Kelly
Feist, Stephen W.
Coates, Christopher J.
Rowley, Andrew F.
author_facet Davies, Charlotte E.
Bass, David
Ward, Georgia M.
Batista, Frederico M.
Malkin, Sophie H.
Thomas, Jessica E.
Bateman, Kelly
Feist, Stephen W.
Coates, Christopher J.
Rowley, Andrew F.
author_sort Davies, Charlotte E.
collection PubMed
description This study provides a morphological and phylogenetic characterization of two novel species of the order Haplosporida (Haplosporidium carcini n. sp., and H. cranc n. sp.) infecting the common shore crab Carcinus maenas collected at one location in Swansea Bay, South Wales, UK. Both parasites were observed in the haemolymph, gills and hepatopancreas. The prevalence of clinical infections (i.e. parasites seen directly in fresh haemolymph preparations) was low, at ~1%, whereas subclinical levels, detected by polymerase chain reaction, were slightly higher at ~2%. Although no spores were found in any of the infected crabs examined histologically (n = 334), the morphology of monokaryotic and dikaryotic unicellular stages of the parasites enabled differentiation between the two new species. Phylogenetic analyses of the new species based on the small subunit (SSU) rDNA gene placed H. cranc in a clade of otherwise uncharacterized environmental sequences from marine samples, and H. carcini in a clade with other crustacean-associated lineages.
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spelling pubmed-74437492020-09-09 Diagnosis and prevalence of two new species of haplosporidians infecting shore crabs Carcinus maenas: Haplosporidium carcini n. sp., and H. cranc n. sp. Davies, Charlotte E. Bass, David Ward, Georgia M. Batista, Frederico M. Malkin, Sophie H. Thomas, Jessica E. Bateman, Kelly Feist, Stephen W. Coates, Christopher J. Rowley, Andrew F. Parasitology Research Article This study provides a morphological and phylogenetic characterization of two novel species of the order Haplosporida (Haplosporidium carcini n. sp., and H. cranc n. sp.) infecting the common shore crab Carcinus maenas collected at one location in Swansea Bay, South Wales, UK. Both parasites were observed in the haemolymph, gills and hepatopancreas. The prevalence of clinical infections (i.e. parasites seen directly in fresh haemolymph preparations) was low, at ~1%, whereas subclinical levels, detected by polymerase chain reaction, were slightly higher at ~2%. Although no spores were found in any of the infected crabs examined histologically (n = 334), the morphology of monokaryotic and dikaryotic unicellular stages of the parasites enabled differentiation between the two new species. Phylogenetic analyses of the new species based on the small subunit (SSU) rDNA gene placed H. cranc in a clade of otherwise uncharacterized environmental sequences from marine samples, and H. carcini in a clade with other crustacean-associated lineages. Cambridge University Press 2020-09 2020-06-16 /pmc/articles/PMC7443749/ /pubmed/32539882 http://dx.doi.org/10.1017/S0031182020000980 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by/4.0/This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Davies, Charlotte E.
Bass, David
Ward, Georgia M.
Batista, Frederico M.
Malkin, Sophie H.
Thomas, Jessica E.
Bateman, Kelly
Feist, Stephen W.
Coates, Christopher J.
Rowley, Andrew F.
Diagnosis and prevalence of two new species of haplosporidians infecting shore crabs Carcinus maenas: Haplosporidium carcini n. sp., and H. cranc n. sp.
title Diagnosis and prevalence of two new species of haplosporidians infecting shore crabs Carcinus maenas: Haplosporidium carcini n. sp., and H. cranc n. sp.
title_full Diagnosis and prevalence of two new species of haplosporidians infecting shore crabs Carcinus maenas: Haplosporidium carcini n. sp., and H. cranc n. sp.
title_fullStr Diagnosis and prevalence of two new species of haplosporidians infecting shore crabs Carcinus maenas: Haplosporidium carcini n. sp., and H. cranc n. sp.
title_full_unstemmed Diagnosis and prevalence of two new species of haplosporidians infecting shore crabs Carcinus maenas: Haplosporidium carcini n. sp., and H. cranc n. sp.
title_short Diagnosis and prevalence of two new species of haplosporidians infecting shore crabs Carcinus maenas: Haplosporidium carcini n. sp., and H. cranc n. sp.
title_sort diagnosis and prevalence of two new species of haplosporidians infecting shore crabs carcinus maenas: haplosporidium carcini n. sp., and h. cranc n. sp.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7443749/
https://www.ncbi.nlm.nih.gov/pubmed/32539882
http://dx.doi.org/10.1017/S0031182020000980
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