Haplotype networks of SARS-CoV-2 infections in the Diamond Princess cruise ship outbreak

The Diamond Princess cruise ship was put under quarantine offshore Yokohama, Japan, after a passenger who disembarked in Hong Kong was confirmed as a coronavirus disease 2019 case. We performed whole-genome sequencing of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) directly from PCR(...

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Detalles Bibliográficos
Autores principales: Sekizuka, Tsuyoshi, Itokawa, Kentaro, Kageyama, Tsutomu, Saito, Shinji, Takayama, Ikuyo, Asanuma, Hideki, Nao, Naganori, Tanaka, Rina, Hashino, Masanori, Takahashi, Takuri, Kamiya, Hajime, Yamagishi, Takuya, Kakimoto, Kensaku, Suzuki, Motoi, Hasegawa, Hideki, Wakita, Takaji, Kuroda, Makoto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2020
Materias:
Biological Sciences
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7443927/
https://www.ncbi.nlm.nih.gov/pubmed/32723824
http://dx.doi.org/10.1073/pnas.2006824117
Descripción
Sumario:The Diamond Princess cruise ship was put under quarantine offshore Yokohama, Japan, after a passenger who disembarked in Hong Kong was confirmed as a coronavirus disease 2019 case. We performed whole-genome sequencing of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) directly from PCR(+) clinical specimens and conducted a phylogenetic analysis of the outbreak. All tested isolates exhibited a transversion at G(11083)T, suggesting that SARS-CoV-2 dissemination on the Diamond Princess originated from a single introduction event before the quarantine started. Although further spreading might have been prevented by quarantine, some progeny clusters could be linked to transmission through mass-gathering events in the recreational areas and direct transmission among passengers who shared cabins during the quarantine. This study demonstrates the usefulness of haplotype network/phylogeny analysis in identifying potential infection routes.

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