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SARS-CoV-2 infection dynamics in lungs of African green monkeys

Detailed knowledge about the dynamics of SARS-CoV-2 infection is important for unraveling the viral and host factors that contribute to COVID-19 pathogenesis. Old-World nonhuman primates recapitulate mild-moderate COVID-19 cases, thereby serving as important pathogenesis models. We compared African...

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Autores principales: Speranza, Emily, Williamson, Brandi N., Feldmann, Friederike, Sturdevant, Gail L., Pérez- Pérez, Lizzette, Mead-White, Kimberly, Smith, Brian J., Lovaglio, Jamie, Martens, Craig, Munster, Vincent J., Okumura, Atsushi, Shaia, Carl, Feldmann, Heinz, Best, Sonja M., de Wit, Emmie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7444286/
https://www.ncbi.nlm.nih.gov/pubmed/32839775
http://dx.doi.org/10.1101/2020.08.20.258087
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author Speranza, Emily
Williamson, Brandi N.
Feldmann, Friederike
Sturdevant, Gail L.
Pérez- Pérez, Lizzette
Mead-White, Kimberly
Smith, Brian J.
Lovaglio, Jamie
Martens, Craig
Munster, Vincent J.
Okumura, Atsushi
Shaia, Carl
Feldmann, Heinz
Best, Sonja M.
de Wit, Emmie
author_facet Speranza, Emily
Williamson, Brandi N.
Feldmann, Friederike
Sturdevant, Gail L.
Pérez- Pérez, Lizzette
Mead-White, Kimberly
Smith, Brian J.
Lovaglio, Jamie
Martens, Craig
Munster, Vincent J.
Okumura, Atsushi
Shaia, Carl
Feldmann, Heinz
Best, Sonja M.
de Wit, Emmie
author_sort Speranza, Emily
collection PubMed
description Detailed knowledge about the dynamics of SARS-CoV-2 infection is important for unraveling the viral and host factors that contribute to COVID-19 pathogenesis. Old-World nonhuman primates recapitulate mild-moderate COVID-19 cases, thereby serving as important pathogenesis models. We compared African green monkeys inoculated with SARS-CoV-2 or inactivated virus to study the dynamics of virus replication throughout the respiratory tract. RNA sequencing of single cells from the lungs and mediastinal lymph nodes allowed a high-resolution analysis of virus replication and host responses over time. Viral replication was mainly localized to the lower respiratory tract, with evidence of replication in the pneumocytes. Macrophages were found to play a role in initiating a pro-inflammatory state in the lungs, while also interacting with infected pneumocytes. Our dataset provides a detailed view of changes in host and virus replication dynamics over the course of mild COVID-19 and serves as a valuable resource to identify therapeutic targets.
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spelling pubmed-74442862020-08-25 SARS-CoV-2 infection dynamics in lungs of African green monkeys Speranza, Emily Williamson, Brandi N. Feldmann, Friederike Sturdevant, Gail L. Pérez- Pérez, Lizzette Mead-White, Kimberly Smith, Brian J. Lovaglio, Jamie Martens, Craig Munster, Vincent J. Okumura, Atsushi Shaia, Carl Feldmann, Heinz Best, Sonja M. de Wit, Emmie bioRxiv Article Detailed knowledge about the dynamics of SARS-CoV-2 infection is important for unraveling the viral and host factors that contribute to COVID-19 pathogenesis. Old-World nonhuman primates recapitulate mild-moderate COVID-19 cases, thereby serving as important pathogenesis models. We compared African green monkeys inoculated with SARS-CoV-2 or inactivated virus to study the dynamics of virus replication throughout the respiratory tract. RNA sequencing of single cells from the lungs and mediastinal lymph nodes allowed a high-resolution analysis of virus replication and host responses over time. Viral replication was mainly localized to the lower respiratory tract, with evidence of replication in the pneumocytes. Macrophages were found to play a role in initiating a pro-inflammatory state in the lungs, while also interacting with infected pneumocytes. Our dataset provides a detailed view of changes in host and virus replication dynamics over the course of mild COVID-19 and serves as a valuable resource to identify therapeutic targets. Cold Spring Harbor Laboratory 2020-08-20 /pmc/articles/PMC7444286/ /pubmed/32839775 http://dx.doi.org/10.1101/2020.08.20.258087 Text en https://creativecommons.org/publicdomain/zero/1.0/This article is a US Government work. It is not subject to copyright under 17 USC 105 and is also made available for use under a CC0 license (https://creativecommons.org/publicdomain/zero/1.0/) .
spellingShingle Article
Speranza, Emily
Williamson, Brandi N.
Feldmann, Friederike
Sturdevant, Gail L.
Pérez- Pérez, Lizzette
Mead-White, Kimberly
Smith, Brian J.
Lovaglio, Jamie
Martens, Craig
Munster, Vincent J.
Okumura, Atsushi
Shaia, Carl
Feldmann, Heinz
Best, Sonja M.
de Wit, Emmie
SARS-CoV-2 infection dynamics in lungs of African green monkeys
title SARS-CoV-2 infection dynamics in lungs of African green monkeys
title_full SARS-CoV-2 infection dynamics in lungs of African green monkeys
title_fullStr SARS-CoV-2 infection dynamics in lungs of African green monkeys
title_full_unstemmed SARS-CoV-2 infection dynamics in lungs of African green monkeys
title_short SARS-CoV-2 infection dynamics in lungs of African green monkeys
title_sort sars-cov-2 infection dynamics in lungs of african green monkeys
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7444286/
https://www.ncbi.nlm.nih.gov/pubmed/32839775
http://dx.doi.org/10.1101/2020.08.20.258087
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