Genotoxic effect of 2,2’-bis(bicyclo[2.2.1] heptane) on bacterial cells

The toxic effect of strained hydrocarbon 2,2'—bis (bicyclo[2.2.1]heptane) (BBH) was studied using whole-cell bacterial lux-biosensors based on Escherichia coli cells in which luciferase genes are transcriptionally fused with stress-inducible promoters. It was shown that BBH has the genotoxic ef...

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Detalles Bibliográficos
Autores principales: Kessenikh, A., Gnuchikh, E., Bazhenov, S., Bermeshev, M., Pevgov, V., Samoilov, V., Shorunov, S., Maksimov, A., Yaguzhinsky, L., Manukhov, I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7444485/
https://www.ncbi.nlm.nih.gov/pubmed/32822344
http://dx.doi.org/10.1371/journal.pone.0228525
Descripción
Sumario:The toxic effect of strained hydrocarbon 2,2'—bis (bicyclo[2.2.1]heptane) (BBH) was studied using whole-cell bacterial lux-biosensors based on Escherichia coli cells in which luciferase genes are transcriptionally fused with stress-inducible promoters. It was shown that BBH has the genotoxic effect causing bacterial SOS response however no alkylating effect has been revealed. In addition to DNA damage, there is an oxidative effect causing the response of OxyR/S and SoxR/S regulons. The most sensitive to BBH lux-biosensor was E. coli pSoxS-lux which reacts to the appearance of superoxide anion radicals in the cell. It is assumed that the oxidation of BBH leads to the generation of reactive oxygen species, which provide the main contribution to the genotoxicity of this substance.

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