Cargando…
The roles of jim lovell and uninflatable in different endopolyploid larval tissues of Drosophila melanogaster
The larvae of Drosophila melanogaster grow rapidly through use of a highly truncated cell cycle in which mitosis is entirely eliminated. The Drosophila homolog of the protooncogene transcription factor Myc plays a major role in promoting this endopolyploid (EP) growth. We have previously determined...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7444548/ https://www.ncbi.nlm.nih.gov/pubmed/32822370 http://dx.doi.org/10.1371/journal.pone.0237662 |
_version_ | 1783573829133533184 |
---|---|
author | Zhou, Fanli Green, Stephanie R. Tsay, Michael Hsu, Safina Dibbs, Rami Beckingham, Kathleen M. |
author_facet | Zhou, Fanli Green, Stephanie R. Tsay, Michael Hsu, Safina Dibbs, Rami Beckingham, Kathleen M. |
author_sort | Zhou, Fanli |
collection | PubMed |
description | The larvae of Drosophila melanogaster grow rapidly through use of a highly truncated cell cycle in which mitosis is entirely eliminated. The Drosophila homolog of the protooncogene transcription factor Myc plays a major role in promoting this endopolyploid (EP) growth. We have previously determined that the gene jim lovell (lov), which encodes a member of the BTB/POZ (Bric-a-brac, Tramtrack, Broad/Pox virus zinc finger) domain family of transcription factors, is also required for EP growth in one larval tissue, the trachea. Here we show that lov promotes EP growth in three further tissues indicating a fundamental role in this process. However, epistasis experiments revealed heterogeneity in lov’s action in these tissues. Whereas in the tracheae and salivary glands lov acts downstream of Myc, in the fat body, reduced expression of lov does not impede the action of Myc, indicating an upstream action for the gene. We show here that lov’s regulation of the gene uninflatable (uif) in the tracheae is a component of this difference. uif is required for tracheal EP growth downstream of Myc and lov but has no equivalent role in the fat body. Although Uif is a transmembrane component of the plasma membrane in the tracheae, its action downstream of Myc suggests an intracellular role for the protein in the tracheae. In addition to regulating uif expression in some tissues we also show that lov locates to the nucleolus, indicating it can function in both polymerase I and polymerase II transcriptional events. Our major finding is that tissue-specific mechanisms can interact with universal growth promotion by Myc to generate the individual endopolyploid organs of the larvae. |
format | Online Article Text |
id | pubmed-7444548 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-74445482020-08-27 The roles of jim lovell and uninflatable in different endopolyploid larval tissues of Drosophila melanogaster Zhou, Fanli Green, Stephanie R. Tsay, Michael Hsu, Safina Dibbs, Rami Beckingham, Kathleen M. PLoS One Research Article The larvae of Drosophila melanogaster grow rapidly through use of a highly truncated cell cycle in which mitosis is entirely eliminated. The Drosophila homolog of the protooncogene transcription factor Myc plays a major role in promoting this endopolyploid (EP) growth. We have previously determined that the gene jim lovell (lov), which encodes a member of the BTB/POZ (Bric-a-brac, Tramtrack, Broad/Pox virus zinc finger) domain family of transcription factors, is also required for EP growth in one larval tissue, the trachea. Here we show that lov promotes EP growth in three further tissues indicating a fundamental role in this process. However, epistasis experiments revealed heterogeneity in lov’s action in these tissues. Whereas in the tracheae and salivary glands lov acts downstream of Myc, in the fat body, reduced expression of lov does not impede the action of Myc, indicating an upstream action for the gene. We show here that lov’s regulation of the gene uninflatable (uif) in the tracheae is a component of this difference. uif is required for tracheal EP growth downstream of Myc and lov but has no equivalent role in the fat body. Although Uif is a transmembrane component of the plasma membrane in the tracheae, its action downstream of Myc suggests an intracellular role for the protein in the tracheae. In addition to regulating uif expression in some tissues we also show that lov locates to the nucleolus, indicating it can function in both polymerase I and polymerase II transcriptional events. Our major finding is that tissue-specific mechanisms can interact with universal growth promotion by Myc to generate the individual endopolyploid organs of the larvae. Public Library of Science 2020-08-21 /pmc/articles/PMC7444548/ /pubmed/32822370 http://dx.doi.org/10.1371/journal.pone.0237662 Text en © 2020 Zhou et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Zhou, Fanli Green, Stephanie R. Tsay, Michael Hsu, Safina Dibbs, Rami Beckingham, Kathleen M. The roles of jim lovell and uninflatable in different endopolyploid larval tissues of Drosophila melanogaster |
title | The roles of jim lovell and uninflatable in different endopolyploid larval tissues of Drosophila melanogaster |
title_full | The roles of jim lovell and uninflatable in different endopolyploid larval tissues of Drosophila melanogaster |
title_fullStr | The roles of jim lovell and uninflatable in different endopolyploid larval tissues of Drosophila melanogaster |
title_full_unstemmed | The roles of jim lovell and uninflatable in different endopolyploid larval tissues of Drosophila melanogaster |
title_short | The roles of jim lovell and uninflatable in different endopolyploid larval tissues of Drosophila melanogaster |
title_sort | roles of jim lovell and uninflatable in different endopolyploid larval tissues of drosophila melanogaster |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7444548/ https://www.ncbi.nlm.nih.gov/pubmed/32822370 http://dx.doi.org/10.1371/journal.pone.0237662 |
work_keys_str_mv | AT zhoufanli therolesofjimlovellanduninflatableindifferentendopolyploidlarvaltissuesofdrosophilamelanogaster AT greenstephanier therolesofjimlovellanduninflatableindifferentendopolyploidlarvaltissuesofdrosophilamelanogaster AT tsaymichael therolesofjimlovellanduninflatableindifferentendopolyploidlarvaltissuesofdrosophilamelanogaster AT hsusafina therolesofjimlovellanduninflatableindifferentendopolyploidlarvaltissuesofdrosophilamelanogaster AT dibbsrami therolesofjimlovellanduninflatableindifferentendopolyploidlarvaltissuesofdrosophilamelanogaster AT beckinghamkathleenm therolesofjimlovellanduninflatableindifferentendopolyploidlarvaltissuesofdrosophilamelanogaster AT zhoufanli rolesofjimlovellanduninflatableindifferentendopolyploidlarvaltissuesofdrosophilamelanogaster AT greenstephanier rolesofjimlovellanduninflatableindifferentendopolyploidlarvaltissuesofdrosophilamelanogaster AT tsaymichael rolesofjimlovellanduninflatableindifferentendopolyploidlarvaltissuesofdrosophilamelanogaster AT hsusafina rolesofjimlovellanduninflatableindifferentendopolyploidlarvaltissuesofdrosophilamelanogaster AT dibbsrami rolesofjimlovellanduninflatableindifferentendopolyploidlarvaltissuesofdrosophilamelanogaster AT beckinghamkathleenm rolesofjimlovellanduninflatableindifferentendopolyploidlarvaltissuesofdrosophilamelanogaster |